Deep Subsurface Microbial Biomass and Community Structure in Witwatersrand Basin Mines

Pfiffner, Susan M.; Cantu, James; Smithgall, Amanda; Peacock, Aaron D.; White, David C.; Moser, Duane P.; Onstott, Tullis C.; Heerden, Esta van

doi:10.1080/01490450600875712

Cited by 43 publications

(30 citation statements)

References 36 publications

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“…Free Fatty Acids (FFAs), phospholipids (PLs) and alkanes were quantified by extraction of pellets from cells harvested at OD 750 = 0.6 by the Bligh-Dyer method [22][23][24][25]. Briefly, 10 ml of a 10:5:4 mixture of methanol:chloroform:pH 7 phosphate buffer was combined with the samples.…”

Section: Gc/ms Analysesmentioning

confidence: 99%

Metabolic phenotyping of the cyanobacterium Synechocystis 6803 engineered for production of alkanes and free fatty acids

Borglin

Kamennaya

et al. 2013

Applied Energy

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Section: Gc/ms Analysesmentioning

confidence: 99%

Metabolic phenotyping of the cyanobacterium Synechocystis 6803 engineered for production of alkanes and free fatty acids

Borglin

Kamennaya

et al. 2013

Applied Energy

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“…The occurrence of microflora in deep, subsurface layers is well documented (Frederikson et al 1991;Lehman et al 2001;Inagaki et al 2002;Borscik et al 2003;Pfiffner et al 2006;Elhottová et al 2006;Fredrickson and Balkwill 2006;Mauclaire et al 2007). These microorganisms contribute to many important geological processes (Pedersen 1993).…”

Section: Introductionmentioning

confidence: 99%

Deep, subsurface microflora after excavation respiration and biomass and its potential role in degradation of fossil organic matter

et al. 2011

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Three types of Miocene claystones (amorphous, lamellar, and transitional) were aseptically sampled from depths of 30 m and 150 m below the soil surface. Respiration of these sediments was measured under conditions that prevented inoculation by other microorganisms not indigenous to the claystones in situ. Microbial respiration was higher in lamellar than amorphous claystones and was not affected by sampling depth. During cultivation, microbial biomass (as indicated by PLFA) significantly increased. Microbial biomass after cultivation was significantly higher in sediments from 30 m than from 150 m depth. Both microbial respiration and biomass increased after glucose addition.

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“…The increasing interest in intra-terrestrial life within subsurface mines may lead us to expand our knowledge on diverse microbial communities with unique metabolic properties [3,5]. In culture experiments of the recent years, independent approaches have been introduced to unravel the metabolic potential of microbial communities inhabiting environments ranging from soil, water, stromatolites, and acid mine drainage to ultra-deep mines [2, 3, 6 -8].…”

mentioning

confidence: 99%

Culture‐dependent and ‐independent molecular analysis of the bacterial community within uranium ore

Islam

Sar

2011

J. Basic Microbiol.

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The bacterial community structure within a uranium ore was investigated using culture-dependent and -independent clone library analysis and denaturing gradient gel electrophoresis of 16S rRNA genes. The major aerobic heterotrophic bacteria were isolated and identified, and their resistance to uranium and other heavy metals was characterized. Together with near neutral pH, moderate organic carbon content, elevated U and other heavy metals (V, Ni, Mn, Cu, etc.), the ore showed high microbial counts and phylotype richness. The bacterial community mainly consisted of uncultured Proteobacteria, with the predominance of γ - over β - and α -subdivisions, along with Actinobacteria and Firmicutes. A phylogenetic study revealed that nearly one-third of the community was affiliated to as yet uncultured and unidentified bacteria having a closer relationship to Pseudomonas. Lineages of Burkholderiaceae and Moraxellaceae were relatively more abundant in the total community, while genera affiliated to Xanthomonadaceae and Microbacteriaceae and Exiguobacterium were detected in the culturable fraction. More than 50% of the bacterial isolates affiliated to Stenotrophomonas, Microbacterium, Acinetobacter, Pseudomonas and Enterobacter showed resistance to uranium and other heavy metals. The study showed for the first time that uranium ore harbors major bacterial groups related to organisms having a wide range of environmentally significant functional attributes, and the most abundant members are possibly new groups/taxa. These findings provide new insights into U-ore geomicrobiology that could be useful in biohydrometallurgy and bioremediation applications.

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Deep Subsurface Microbial Biomass and Community Structure in Witwatersrand Basin Mines

Cited by 43 publications

References 36 publications

Metabolic phenotyping of the cyanobacterium Synechocystis 6803 engineered for production of alkanes and free fatty acids

Metabolic phenotyping of the cyanobacterium Synechocystis 6803 engineered for production of alkanes and free fatty acids

Deep, subsurface microflora after excavation respiration and biomass and its potential role in degradation of fossil organic matter

Culture‐dependent and ‐independent molecular analysis of the bacterial community within uranium ore

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