Deepening our understanding of bioactive glass crystallization using TEM and 3D nano-CT

Jaimes, Altair T. Contreras; Pablos-Martín, A. de; Hurle, Katrin; Silva, Juliana Martins de Souza e; Berthold, Lutz; Kittel, Thomas; Boccaccını, Aldo R.; Brauer, Delia S.

doi:10.1016/j.jeurceramsoc.2021.02.051

Cited by 20 publications

(10 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 g) may have a second contribution from diffraction contrast in addition to the Z-contrast. Diffraction contrast originates from differences in the orientation of the atomic layers rather than density or chemical composition (similar to what we observed previously for Bioglass 45S5 36 ). Both contrast contributions can be explained as very early stages of crystallisation, i.e.…”

Section: Resultssupporting

confidence: 86%

Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses

Jaimes

Kirste

Pablos-Martín

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

Bioactive glasses convert to a biomimetic apatite when in contact with physiological solutions; however, the number and type of phases precipitating depends on glass composition and reactivity. This process is typically followed by X-ray diffraction and infrared spectroscopy. Here, we visualise surface mineralisation in a series of sodium-free bioactive glasses, using transmission electron microscopy (TEM) with energy-dispersive X-ray spectroscopy (EDXS) and X-ray nano-computed tomography (nano-CT). In the glasses, the phosphate content was increased while adding stoichiometric amounts of calcium to maintain phosphate in an orthophosphate environment in the glass. Calcium fluoride was added to keep the melting temperature low. TEM brought to light the presence of phosphate clustering and nearly crystalline calcium fluoride environments in the glasses. A combination of analytical methods, including solid-state NMR, shows how with increasing phosphate content in the glass, precipitation of calcium fluoride during immersion is superseded by fluorapatite precipitation. Nano-CT gives insight into bioactive glass particle morphology after immersion, while TEM illustrates how compositional changes in the glass affect microstructure at a sub-micron to nanometre-level.

show abstract

Section: Resultssupporting

confidence: 86%

Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses

Jaimes

Kirste

Pablos-Martín

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is also evident that nano-CT provides better 3D spatial visualization than conventional micro-CT, SEM, or AFM methods [ 139 ]. It offers insights into the morphology (e.g., crystallinity and porosity) of bioactive materials prepared under different conditions [ 140 , 141 , 142 ] and can be used to evaluate bone growth after implantation, similarly to micro-CT examinations. In an in vivo study carried out by Cuijpers et al [ 143 ], the authors compared micro- and nano-CT techniques for the assessment of newly formed bone tissue after the implantation of Ti-coated PMMA implants.…”

Section: Interaction and Morphology Studiesmentioning

confidence: 99%

Analytical Techniques for the Characterization of Bioactive Coatings for Orthopaedic Implants

Kravanja

Finšgar

2021

Biomedicines

View full text Add to dashboard Cite

The development of bioactive coatings for orthopedic implants has been of great interest in recent years in order to achieve both early- and long-term osseointegration. Numerous bioactive materials have been investigated for this purpose, along with loading coatings with therapeutic agents (active compounds) that are released into the surrounding media in a controlled manner after surgery. This review initially focuses on the importance and usefulness of characterization techniques for bioactive coatings, allowing the detailed evaluation of coating properties and further improvements. Various advanced analytical techniques that have been used to characterize the structure, interactions, and morphology of the designed bioactive coatings are comprehensively described by means of time-of-flight secondary ion mass spectrometry (ToF-SIMS), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), 3D tomography, quartz crystal microbalance (QCM), coating adhesion, and contact angle (CA) measurements. Secondly, the design of controlled-release systems, the determination of drug release kinetics, and recent advances in drug release from bioactive coatings are addressed as the evaluation thereof is crucial for improving the synthesis parameters in designing optimal bioactive coatings.

show abstract

“…Nano‐CT provides resolution down to 50 nm which can monitor early‐stage microstructural developments in glass‐ceramics 73 . Nano‐CT has recently been employed in studying the Bioglass ® 45S5 system to examine the structural development of the combeite crystalline phase as a function of heat treatment schedules 74,75 . In‐situ ESEM has been used in several studies to image the crystallization and sintering of bioactive glasses in real time 76,77 …”

Section: Controlled Crystallizationmentioning

confidence: 99%

Perspectives on the impact of crystallization in bioactive glasses and glass‐ceramics

Montazerian,

Shearer,

Mauro

2023

Int J Ceramic Engine & Sci

View full text Add to dashboard Cite

Both unwanted and induced crystallization can impact bioactivity, physical and mechanical properties of bioactive glasses (BGs). Uncontrolled crystallization has negative consequences, rendering BGs unreliable. However, by manipulating the type, size, shape, and quantity of crystals in BGs, plenty of opportunities arise for controlling, for example, mechanical properties and degradability, leading to unique applications and improved performance. Understanding crystallization is a key step in developing bioactive glasses and glass‐ceramics (BGCs), and both fundamental and experimental research can aid in the design of BGCs for processing and biological function. In this perspective, we discuss the sources of crystallization and how controlled crystallization facilitates the functionalization of bioactive scaffolds, hybrids, coatings, composites, cements, and fibers.

show abstract

Deepening our understanding of bioactive glass crystallization using TEM and 3D nano-CT

Cited by 20 publications

References 67 publications

Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses

Nano-imaging confirms improved apatite precipitation for high phosphate/silicate ratio bioactive glasses

Analytical Techniques for the Characterization of Bioactive Coatings for Orthopaedic Implants

Perspectives on the impact of crystallization in bioactive glasses and glass‐ceramics

Contact Info

Product

Resources

About