DeepGOMeta: Predicting functions for microbes

Abstract

Analyzing microbial samples remains computationally challenging due to their diversity and complexity. The lack of robustde novoprotein function prediction methods exacerbates the difficulty in deriving functional insights from these samples. Traditional prediction methods, dependent on homology and sequence similarity, often fail to predict functions for novel proteins and proteins without known homologs. Moreover, most of these methods have been trained on largely eukaryotic data, and have not been evaluated or applied to microbial datasets. This research introduces DeepGOMeta, a deep learning model designed for protein function prediction, as Gene Ontology (GO) terms, trained on a dataset relevant to microbes. The model is validated using novel evaluation strategies and applied to diverse microbial datasets. Data and code are available athttps://github.com/bio-ontology-research-group/deepgometa