Defatted Jatropha curcas flour and protein isolate as materials for protein hydrolysates with biological activity

Marrufo-Estrada, Duly M.; Campos, Maira Rubí Segura; Chel‐Guerrero, Luis; Betancur‐Ancona, David

doi:10.1016/j.foodchem.2012.09.033

Cited by 39 publications

(32 citation statements)

References 27 publications

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“…is within the range reported by Marrufo-Marrufo- Estrada et al (2013), who carried out a chemical analysis for amount of crude protein and fiber, where protein content was 28%. Similar values on percentage of protein (Martínez et al, 2006;Valdes-Rodriguez et al, 2013) are reported for seeds from the state of Veracruz.…”

Section: Resultssupporting

confidence: 85%

“…Esto debido a que la enzima oleil-Coa-desaturasa disminuye su actividad, provocando que la relación oleico-linoleico aumente (Garcés et al, 1992). La concentración de proteína encontrado esta entre 19 y 27%, está dentro del intervalo reportado por Marrufo-Marrufo- Estrada et al (2013), quienes realizaron un análisis químico para determinar cantidad de proteína cruda y fibra, donde el contenido de proteína fue de 28%. Para semillas provenientes del estado de Veracruz, se reportan valores similares de porcentaje de proteína (Makkar y Becker, 1997;Martínez et al, 2006;Valdés-Rodríguez et al, 2013).…”

Section: Resultsunclassified

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Análisis químico-morfológico comparativo de accesiones de Jatropha curcas L. del estado de Veracruz

Rubio

Pérez-Vázquez

Pérez

et al. 2017

Remexca

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El biodiesel producido a partir de semillas de Jatropha curcas es técnicamente viable, aunque existe poca experiencia a nivel nacional. Se desconoce si existe variación en los contenidos químicos de las semillas dependiendo del sitio de crecimiento (interacción genotipo-ambiente). Por tanto, el objetivo del presente estudio fue comparar la variación en características morfológicas y las variaciones en el contenido de aceite, proteína, esteres de forbol, índice de acidez, ácido oleico e índice de saponificación, en semillas de J. curcas recolectadas en diferentes zonas agroecológicas del estado de Veracruz y su réplica establecida en el Banco de Germoplasma (BG) del Campus Veracruz. Las semillas fueron recolectadas en agosto de 2013. Para los análisis químicos se usaron técnicas aprobadas por la AOAC. Para los análisis estadísticos se usó el programa Statistic versión 7 y Excel 2010. Se encontró una correlación significativa (r≤ 1) entre las variables morfológicas de las semillas, particularmente entre la longitud y peso (r≤ 0.75); la menor correlación fue entre ancho y largo (p≤ 0.36). El análisis de correlación entre el contenido de aceite y peso de semillas no fue significativo (p≤ 0.38). El promedio de aceite en Campo y BG fue de 38.5±5.8, de ácido oleico36±4.2, índice de saponificación 194±1.7, índice de acidez7.1±0.8, proteína24.3±3.7, esteres de forbol 0.34±1.04. Se concluye que hubo diferencias significativas para la variable esteres de forbol en las condiciones de campo y BG, siendo mayores las concentraciones en las semillas de campo.

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Section: Resultssupporting

confidence: 85%

Section: Resultsunclassified

Análisis químico-morfológico comparativo de accesiones de Jatropha curcas L. del estado de Veracruz

Rubio

Pérez-Vázquez

Pérez

et al. 2017

Remexca

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“…Hence, there is the need for its replacement with a potential non-conventional plant protein sources for cost efficient and sustainable aquafeed development. Therefore, in the present scenario, non-edible oil seeds cake or kernel would be one of the most preferred choice provided they are made free of deterrent factors, and nutritionally compatible to cultured species (Marrufo-Estrada et al, 2013;Suprayudi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Extraction of protein from defatted seed cake has been described as a way of reducing the contents of antinutrient and toxic components (Marrufo-Estrada et al, 2013) with high levels of protein, which often have digestibility similar or greater than that of fishmeal protein (Makkar et al, 2008). Since rubber kernel meal contain less protein, a large amount of indigestible material and anti-nutrients, there is need to convert them into a better useful products by isolating the protein for optimum utilization.…”

Section: Introductionmentioning

confidence: 99%

Nutritional evaluation of protein isolate from rubber seed in the diet of Labeo rohita : Effects on growth performance, nutrient utilization, whole body composition and metabolic enzymes activity

Fawole

Sahu

Jain

et al. 2016

Animal Feed Science and Technology

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a b s t r a c tThe nutritional potential of protein isolate from rubber seed (RPI) in the diets of Labeo rohita (initial average weight 4.45 ± 0.01 g) was assessed in a 60 days feeding trial. Five isonitrogenous (32.62 ± 0.13 CP Kg −1 ) and isocaloric (18.47 ± 0.08 MJ kg −1 ) experimental diets were formulated with graded level of RPI like 0%, 25%, 50%, 75%, or 100% in replacement for soybean protein isolate (SPI), and designated as Control, RPI25, RPI50, RPI75, RPI100, respectively. The RPI contributed 0%, 13%, 26%, 39% or 52% of the total dietary protein in the diets. Each diets were randomly assigned to 15 experimental tanks containing 12 fish in triplicates and fed to satiation twice daily at 10:00 h and 18:00 h. At the end of the feeding trial, the growth performance and nutrient utilization indices such as percent weight gain (WG%), specific growth rate (SGR), daily growth coefficient (DGC), feed intake (FI), protein efficiency ratio (PER), feed conversion ratio (FCR) and protein retention (PR) values were not significantly (p > 0.05) affected by the dietary treatments irrespective of inclusion levels of RPI. A significantly higher (p < 0.05) hepatosomatic index (HSI) was recorded in the control and RPI 50 group compared to other treatment groups (p < 0.05). The intestinal somatic index (ISI) and Survival rate were similar (p > 0.05) in all the groups. The apparent digestibility coefficients (ADCs) of dry matter and protein for fish fed the control and RPI 100 diets were found to be similar, while RPI 50 and RPI 75 groups exhibited a significantly lower value corresponding to the protease enzyme activity. The whole body compositions and digestive/metabolic enzymes activities among the various groups did not differ significantly (p > 0.05). The serum cholesterol and triglyceride levels were found to be significantly higher (p < 0.05) in the control compared to the RPI fed groups. Significantly higher serum glucose level was recorded in RPI 50, while a reverse was seen in the liver glycogen contents. Overall, this study clearly showed that RPI from rubber seed can serve as alternative protein source in the diets of L. rohita fingerlings without any adverse effects on growth, nutrient utilization and physio-metabolic responses.

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“…Oil extraction of PC resulted in an increase in protein concentration to 27.32% due to lowering oil content. The protein content in DF flour of nyamplung was lower compare to DF of castor beans 65.60% [14]. But it was higher than DF of kenaf seeds (26.19%) [15].…”

Section: Resultsmentioning

confidence: 73%

Hydrolysis of Nyamplung (Calophylluminophyllum) protein and their antioxidant activity

Yanti¹,

Hastuti²,

Sulistyo³

et al. 2016

AIP Conference Proceedings

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Abstract. The seedcake of nyamplung seeds still contains high protein. Recent studies show that protein hydrolysate has many useful effects, such as an antioxidant. The protein was isolated by the isoelectric precipitation method, further on hydrolysed into short chain peptides. The chemical composition of seedcake and the protein content of extract were analysed. The hydrolysis of the extract was carried out in a various condition such as substrate concentration, pH, and temperature for a different length of hydrolysis and the degree of hydrolysis was determine by TNBS method. The protein isolation process performed on the defatted seedcake produced 52.84 ± 0.07% of protein extract with a brownish colour. The degree of hydrolysis (DH) was increased by increasing the length of hydrolysis and showed the optimum DH with 6.15mg/mL substrate concentration. The increase of temperature also increased the DH, however at 70°C with 240 min time reaction, the DH tended to decrease. The pH of reaction between papain showed the similar DH among various pH of the reaction, with pH 7 showed the stable increasing DH till 240 min of reaction. The antioxidant activity showed an increase to 90% of inhibition by the addition of the hydrolysate to the system until 1300 ppm. However, the addition of more than 1300 ppm showed a reduction activity

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Defatted Jatropha curcas flour and protein isolate as materials for protein hydrolysates with biological activity

Cited by 39 publications

References 27 publications

Análisis químico-morfológico comparativo de accesiones de Jatropha curcas L. del estado de Veracruz

Análisis químico-morfológico comparativo de accesiones de Jatropha curcas L. del estado de Veracruz

Nutritional evaluation of protein isolate from rubber seed in the diet of Labeo rohita : Effects on growth performance, nutrient utilization, whole body composition and metabolic enzymes activity

Hydrolysis of Nyamplung (Calophylluminophyllum) protein and their antioxidant activity

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