Defective Acetylcholine Receptor Subunit Switch Precedes Atrophy of Slow-Twitch Skeletal Muscle Fibers Lacking ERK1/2 Kinases in Soleus Muscle

Wang, Shuo; Seaberg, Bonnie; Paez-Colasante, Ximena; Rimer, Mendell

doi:10.1038/srep38745

Cited by 8 publications

(13 citation statements)

References 45 publications

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“…This result suggested that ERK1/2 might have a role in maintaining myofiber mass in vivo [5]. Consistent with these results, developmental selective abrogation of Erk1 and Erk2 in myofibers induced muscle fiber loss and atrophy, with stronger effects in the type1-fiber-rich soleus (SOL) than in type 2-fiber-rich tibialis anterior (TA) and sternomastoid (STN) [6,7]. However, sustained ERK activation via selective myofiber expression of an MEK1 constitutively active mutant [8] or a Ras mutant that only activates the MEK-ERK1/2 pathway and not the PI3K-Akt pathway [9], failed to induce fiber hypertrophy.…”

Section: Erk1/2supporting

confidence: 67%

Extracellular signal-regulated kinases 1 and 2 regulate neuromuscular junction and myofiber phenotypes in mammalian skeletal muscle

Rimer

2020

Neuroscience Letters

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The neuromuscular junction is the synapse between a motor neuron of the spinal cord and a skeletal muscle fiber in the periphery. Reciprocal interactions between these excitable cells, and between them and others cell types present within the muscle tissue, shape the development, homeostasis and plasticity of skeletal muscle. An important aim in the field is to understand the molecular mechanisms underlying these cellular interactions, which include identifying the nature of the signals and receptors involved but also of the downstream intracellular signaling cascades elicited by them. This review focuses on work that shows that skeletal muscle fiber-derived extracellular signal-regulated kinases 1 and 2 (ERK1/2), ubiquitous and prototypical intracellular mitogen-activated protein kinases, have modulatory roles in the maintenance of the neuromuscular synapse and in the acquisition and preservation of fiber type identity in skeletal muscle.

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Section: Erk1/2supporting

confidence: 67%

Extracellular signal-regulated kinases 1 and 2 regulate neuromuscular junction and myofiber phenotypes in mammalian skeletal muscle

Rimer

2020

Neuroscience Letters

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“…2 c). No histological sign of denervation was observed, which was confirmed by following the expression of a set of genes known to be upregulated in the case of denervation (AChR-γ; Musk; Myogenin; Runx1) [ 46 ]. RT-q-PCR amplification of these genes showed that their expression was not significantly increased in MAP6 KO muscle compared to WT (Fig.…”

Section: Resultsmentioning

confidence: 99%

Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction

et al. 2018

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BackgroundThe skeletal muscle fiber has a specific and precise intracellular organization which is at the basis of an efficient muscle contraction. Microtubules are long known to play a major role in the function and organization of many cells, but in skeletal muscle, the contribution of the microtubule cytoskeleton to the efficiency of contraction has only recently been studied. The microtubule network is dynamic and is regulated by many microtubule-associated proteins (MAPs). In the present study, the role of the MAP6 protein in skeletal muscle organization and function has been studied using the MAP6 knockout mouse line.MethodsThe presence of MAP6 transcripts and proteins was shown in mouse muscle homogenates and primary culture using RT-PCR and western blot. The in vivo evaluation of muscle force of MAP6 knockout (KO) mice was performed on anesthetized animals using electrostimulation coupled to mechanical measurement and multimodal magnetic resonance. The impact of MAP6 deletion on microtubule organization and intracellular structures was studied using immunofluorescent labeling and electron microscopy, and on calcium release for muscle contraction using Fluo-4 calcium imaging on cultured myotubes. Statistical analysis was performed using Student’s t test or the Mann-Whitney test.ResultsWe demonstrate the presence of MAP6 transcripts and proteins in skeletal muscle. Deletion of MAP6 results in a large number of muscle modifications: muscle weakness associated with slight muscle atrophy, alterations of microtubule network and sarcoplasmic reticulum organization, and reduction in calcium release.ConclusionAltogether, our results demonstrate that MAP6 is involved in skeletal muscle function. Its deletion results in alterations in skeletal muscle contraction which contribute to the global deleterious phenotype of the MAP6 KO mice. As MAP6 KO mouse line is a model for schizophrenia, our work points to a possible muscle weakness associated to some forms of schizophrenia.Electronic supplementary materialThe online version of this article (10.1186/s13395-018-0176-8) contains supplementary material, which is available to authorized users.

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“…Since the activation of the ERK1/2 pathway [ 194 , 204 ] is necessary for normal neuromuscular junctions [ 358 , 359 ], therefore, an intriguing possibility is that the transport-independent signaling functions of PIT1/2 are important for skeletal muscle function [ 204 , 333 ].…”

Section: Importance Of Dietary Phosphorus For Skeletal Muscle Heamentioning

confidence: 99%

Importance of Dietary Phosphorus for Bone Metabolism and Healthy Aging

Serna

Bergwitz

2020

Nutrients

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Inorganic phosphate (Pi) plays a critical function in many tissues of the body: for example, as part of the hydroxyapatite in the skeleton and as a substrate for ATP synthesis. Pi is the main source of dietary phosphorus. Reduced bioavailability of Pi or excessive losses in the urine causes rickets and osteomalacia. While critical for health in normal amounts, dietary phosphorus is plentiful in the Western diet and is often added to foods as a preservative. This abundance of phosphorus may reduce longevity due to metabolic changes and tissue calcifications. In this review, we examine how dietary phosphorus is absorbed in the gut, current knowledge about Pi sensing, and endocrine regulation of Pi levels. Moreover, we also examine the roles of Pi in different tissues, the consequences of low and high dietary phosphorus in these tissues, and the implications for healthy aging.

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Defective Acetylcholine Receptor Subunit Switch Precedes Atrophy of Slow-Twitch Skeletal Muscle Fibers Lacking ERK1/2 Kinases in Soleus Muscle

Cited by 8 publications

References 45 publications

Extracellular signal-regulated kinases 1 and 2 regulate neuromuscular junction and myofiber phenotypes in mammalian skeletal muscle

Extracellular signal-regulated kinases 1 and 2 regulate neuromuscular junction and myofiber phenotypes in mammalian skeletal muscle

Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction

Importance of Dietary Phosphorus for Bone Metabolism and Healthy Aging

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