Defective maintenance of T cell tolerance to a superantigen in MRL-lpr/lpr mice.

Abstract

SummaryIn normal mice neonatal injection of staphylococcal enterotoxin B (SEB) induces tolerance in T cells that express reactive T cell receptor (TCK) V/~ regions. To determine if a T cell neonatal defect was present in MRL-Ipr/Ipr mice, 20 #g of SEB was injected intraperitoneally every other day into VBS.2 TCR transgenic and nontransgenic MR.b +/+ and MKL-Ipr/Ipr mice from birth to 2 wk of age. At 2 wk of age, V~8 + T cells were depleted, and SEB reactivity was lost, in spleen, lymph node, and thymus. These effects were equivalent in +/+ and Ipr/lpr SEB-tolerized mice. However, MILL-Ipr/Ipr mice failed to maintain neonatal tolerance. By 4 wk of age, there was a dramatic increase in T cells expressing V~8.2 in the peripheral lymph nodes of MRblpr/lt,r mice but not MRD +/+ mice. In vitro stimulation with SEB or TCR crosslinking revealed a total loss of neonatal tolerance 2 wk after cessation of SEB treatment in Ipr/Ipr mice, but not +/+ mice. The time-course of recovery of V~8 + T cells and reactivity to SEB and TCR crosslinking in the thymus of MRL-Ipr/Ipr mice was similar to that in the lymph node. Thymectomy at 2 wk of age eliminated tolerance loss in lymph nodes of MtLL-Ipr/Ipr mice at 4 wk of age, indicating that loss of peripheral tolerance was due to the emigration of untolerized T cells from the thymus. Challenge of neonatally tolerized MRb.lpr/Ipr mice with SEB (100 #g, i.p.) at 8 wk of age resulted in a dramatic onset of T cell-mediated autoimmune disease characterized by 30% weight loss and 60% morality. This indicated that loss of tolerance to SEB also occurred in vivo. In contrast, neonatally tolerized MRL-+ / + mice remained totally unresponsive to SEB challenge and did not undergo any detectable weight loss. These results suggest that there is normal induction of neonatal tolerance to SEB in lpr/Ipr mice, but that tolerance is not maintained after the tolerizing antigen is removed. This loss of neonatal tolerance can lead to severe weight loss and death on exposure to the tolerizing antigen later in life. In MRblpr/Ipr mice, which develop lymphadenopathy and autoimmune disease, extensive studies suggest that abnormal thymic development results in release of increased numbers of T cells from the thymus before tolerance induction, leading to excessive numbers of self-reactive T cells in peripheral lymphoid organs (1). This is supported by the recent finding that the Ipr gene is a mutation in the Fas antigen, which normally induces apoptosis (2--4). Although autoreactive T cells are thought to play a prominent role in the development of autoimmune disease, it is unclear if the persistence of autoreactive T cells results from a defect in induction of functional elimination either by clonal deletion or anergy induction, or from a defect in the maintenance of anergy. The present experiments were designed to study directly defects in induction and maintenance of T cell tolerance in MRL-lpr/Ipr mice. Antibodies and Reagents. Anti-CD4 (clone GK1.5), anti-CD8(clone 53-6.77), anti-CD3 (clone 145-2Cll), an...

The Critical Role of Protein Kinase C-θ in Fas/Fas Ligand-Mediated Apoptosis

“…1b). As previously reported (11,30), SEB treatment of WT mice (solid black line) resulted in a rapid increase (expansion phase) of V␤8 ϩ CD4 ϩ T cells from 15 to 40% within 3 days. The ϩ CD4 ϩ (black line) and V␤6 ϩ CD4 ϩ (gray line) T cells was determined using a flow cytometer at 0, 3, 6, 9, and 12 days after challenging the mice.…”

Stabilized β-Catenin Potentiates Fas-Mediated T Cell Apoptosis

“…1b). As previously reported (11,25), SEB treatment of WT mice (dashed line) resulted in a rapid increase (expansion phase) of V␤8 ϩ CD4 ϩ T cells from ϳ20 to 35% within 3 days. The expansion phase was followed by a decrease (deletion phase) in the percentage of V␤8 ϩ CD4 ϩ T cells, leading to the reduction of the V␤8 ϩ CD4 ϩ T cells to ϳ20%.…”

Resistance of T-cells to apoptosis in autoimmune diabetic (NOD) mice is increased early in life and is associated with dysregulated expression of Bcl-x

“…In the model of experimental allergic encephalomyelitis, recovery from disease is closely associated with specific elimination by apoptosis of autoreactive CD4 + T cells in the central nervous system [20±24]. In mice bearing the ipr mutation, elimination of specific T-cell clones following in vivo neonatal injection of a superantigen is incomplete and of short duration [25]. Finally, in mice bearing a Bcl-2 transgene, the antibody response following antigen stimulation is prolonged [26].…”