Defective ristocetin and bovine factor VIII-induced platelet aggregation in normal rats

Gaetano, Giovanni de; Donati, Maria Benedetta; Innocenti, I. Reyers-Degli; Roncaglioni, Maria Carla

doi:10.1007/bf02026403

Cited by 19 publications

(2 citation statements)

References 24 publications

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“…In 1973, we established ex novo a small research group on thrombosis with a very young technician (who is still successfully doing research) and a Polish medical fellow (who later was appointed as Pro Rector of his University) [3][4][5][6]. The context was the Mario Negri Institute for Pharmacological Research in Milan (Table 1).…”

Section: Bio-medical Research: a Choice For The Personmentioning

confidence: 99%

Bio-medical research and search for personhood: a 40-year project

Donati

Gaetano

2012

J Med Pers

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During our PhD thesis in Leuven (Belgium), we chose the path of biomedical research, not as an intellectual enterprise, but as a concrete full time choice for the person. We worked at Mario Negri, Milan, a non-profit institution, convinced that independent research should offer health benefit to the population, rather than economically exploiting the scientific results obtained. Science communication was also developed as an ethic aspect of research. At Mario Negri Sud, located in a small village far from large cities, we combined excellence in research and attention to the surrounding territory, including schools, elderly people, agricultural products, industry and religious traditions. One of the most consistent features of our research work was to involve young fellows and give them responsibility. A major effort was also performed to diffuse a scientific attitude among young people (e.g. ''Summer Student'' program). When we moved further down to Southern Italy, namely to Campobasso, human population studies were fully developed. Being in Molise, our main research project had direct impact on the Molisani people. The ''MOLI-SANI'' project was performed in collaboration with local GPs, regional health authorities, priests in charge of parishes, elderly cultural circles, lay press and television networks, schools. Health promotion, life style and prevention were and still are the preferred targets of the project, rather than diseases, hospitals and drugs. Along whole our life, we believed in research as a preferential way to develop the personhood. If only one of the researchers who worked with us will keep the same faith, we shall feel privileged.

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Section: Bio-medical Research: a Choice For The Personmentioning

confidence: 99%

Bio-medical research and search for personhood: a 40-year project

Donati

Gaetano

2012

J Med Pers

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“…Platelet aggregation was studied by the photometric technique of Born and Cross [1] using a MK IV Born-Michal aggregometer (Pharmacological Research, Cambridge, UK) connected to a two-channel potentiometer (Venture Servoscribe 2, Smiths Industries, Ltd., London, UK), as previously described [9], Separation of platelets from plasma pro teins was obtained by gel filtration, as previously described [12].…”

Section: Methodsmentioning

confidence: 99%

Ditazole and Platelets

Gaetano

Tonolli

Bertoni

et al. 1977

Pathophysiol Haemos Thromb

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Ditazole (4,5-diphenyl-2-bis-(2-hydroxyethyl)-aminoxazol) has been shown to be a strong in vitro inhibitor of human platelet aggregation brought about by release reaction inducers; in contrast, it did not significantly affect primary ADP-induced aggregation. Ditazole strongly inhibited the release of platelet-bound ¹⁴C-serotonin under the influence of Thrombofax, whereas it did not interfere with the transport and storage of serotonin in nonstimulated platelets. The effect of ditazole was not potentiated by acetyl-salicylic acid. Ditazole also inhibited ADP-reptilase clot retraction and modified thrombin-induced clot formation. The inhibition of platelet aggregation exerted by ditazole in plasma could be removed following gel filtration of platelets on Sepharose 2-B gel. This would indicate that ditazole does not act on platelets by a ‘hit and run’ mechanism.

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Borate and glycerol inhibition of the aggregation of guinea pig platelets by bovine factor VIII and ristocetin

Kuwahara

1977

American J Hematol

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To demonstrate the importance of the carbohydrate portions of bovine factor VIII and ristocetin for the induction of platelet aggregation, guinea pig platelets were treated with either borate or glycerol before and after addition of ristocetin or bovine factor VIII. Both borate and glycerol were capable of inhibiting the aggregation of blood platelets induced by adding bovine factor VIII or ristocetin to platelet-rich plasma. Furthermore, aggregates formed after treatment with either agent could be dispersed by high concentrations of either borate or glycerol. These effects did not appear to be solely the result of high ionic strength or of irreversible damage to the platelets. The results obtained support the hypothesis that the binding of the carbohydrate groups of factor VIII plays an important role in the induction of platelet aggregation and in the maintenance of aggregates formed in response to the treatment of platelet-rich plasma with bovine factor VIII or ristocetin.

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Defective ristocetin and bovine factor VIII-induced platelet aggregation in normal rats

Cited by 19 publications

References 24 publications

Bio-medical research and search for personhood: a 40-year project

Bio-medical research and search for personhood: a 40-year project

Ditazole and Platelets

Borate and glycerol inhibition of the aggregation of guinea pig platelets by bovine factor VIII and ristocetin

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