Deferoxamine/magnesium modified β-tricalcium phosphate promotes the bone regeneration in osteoporotic rats

Abstract

Recently, Deferoxamine (DFO) and magnesium (Mg) have been identified as critical factors for angiogenesis and bone formation. However, in current research studies, there is a lack of focus on whether DFO plus Mg can affect the regeneration of β-tricalcium phosphate (β-TCP) in osteoporosis and through what biological mechanisms. Therefore, the present work was aimed to preparation and evaluate the effect of Deferoxamine/magnesium modified β-tricalcium phosphate promotes (DFO/Mg-TCP) in ovariectomized rats model and preliminary exploration of possible mechanisms. The MC3T3-E1 cells were co-cultured with the exudate of DFO/Mg-TCP and induced to osteogenesis, and the cell viability, osteogenic activity were observed by Cell Counting Kit-8(CCK-8), Alkaline Phosphatase (ALP) staining, Alizarin Red Staining (RES) and Western Blot. In vitro experiments, CCK-8, ALP and ARS staining results show that the mineralization and osteogenic activity of MC3T3-E1increased significantly after intervention by DFO/Mg-TCP, as well as a higher levels of protein expressions including VEGF, OC, Runx-2 and HIF-1α. In vivo experiment, Micro-CT and Histological analysis evaluation show that DFO/Mg-TCP treatment presented the stronger effect on bone regeneration, bone mineralization and biomaterial degradation, when compared with OVX+Mg-TCP group and OVX+TCP group, as well as a higher VEGF, OC, Runx-2 and HIF-1α gene expression. The present study indicates that treatment with DFO/Mg-TCP was associated with increased regeneration by enhancing the function of osteoblasts in an OVX rat.

Hypoxia Pathway in Osteoporosis: Laboratory Data for Clinical Prospects

“…Hypoxia or CoCl 2 exposure significantly increased the level of HIF-1α protein and VEGF gene expression, but owned different mechanisms [ 127 ]. DFO was an iron chelator that already currently licensed promoting angiogenesis and bone formation, and also relieved the symptoms of osteoporosis [ 128 ]. DMOG [ 129 ] promoted the proliferation and differentiation of OB by inhibiting PHD activity to induce the HIF pathway.…”

The effect of Nanocrystalline Hydroxyapatite -parathyroid hormone mixture on bone defect healing: Experimental Study in Dogs

“…They are mostly osteoconductive and have no influence on osteogenesis or osteoinduction. (6)(7)(8)(9) Nanomaterials have recently become popular in the medical field. When employed for bone regeneration operations, they have considerable surface, quantum, and size effects, which contribute to superior performance than standard materials.…”