Deferoxamine Preconditioning of Irradiated Tissue Improves Perfusion and Fat Graft Retention

Flacco, John; Chung, Natalie N.; Blackshear, Charles P.; Irizarry, Dre; Momeni, Arash; Lee, Gordon K.; Nguyen, Dung; Gurtner, Geoffrey C.; Longaker, Michael T.; Wan, Derrick C.

doi:10.1097/prs.0000000000004167

Cited by 48 publications

(40 citation statements)

References 39 publications

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“…Prior studies in mice have shown promise for use of DFO in the treatment of RIF 38 . Serial injections of DFO into irradiated tissue prior to fat grafting was observed to increase perfusion in the overlying skin and improve retention of fat graft volumes, thereby mitigating some of the detrimental fibrotic effects of radiation therapy.…”

Section: Discussionmentioning

confidence: 99%

“…Mice were randomized into four experimental groups on the first day of patch placement: (1) IR alone (IR only, n = 8), (2) DFO treatment for two weeks after recovery from IR (DFO post-IR, n = 4), (3) DFO prophylaxis with treatment through and post-IR (DFO ppx, n = 8), or (4) no irradiation or DFO (No IR, n = 4). Mice in the DFO post-IR group were treated with DFO for two weeks, beginning after a 4-week recovery period following completion of IR, adapted from a protocol described by Flacco et al 38 . Patches were changed daily for the 14-day treatment period.…”

Section: Animals Female Adult 60-day-old Cd-1 Nude Immunocompromisedmentioning

confidence: 99%

“…Patches were changed daily for the 14-day treatment period. Dosage delivery with each patch was also determined based on this previously published work 38 . Prophylactic DFO treatment began 2 weeks prior to the initiation of IR and continued until mice were sacrificed.…”

Section: Animals Female Adult 60-day-old Cd-1 Nude Immunocompromisedmentioning

confidence: 99%

“…Sample size was calculated using power analysis, with a minimum sample size of 4 per group needed to detect a difference of 10 µm in skin perfusion between DFO-treated and control-treated groups with a power of 80% at a significant level of 0.05. This calculation was based on our prior work on the effects of DFO in irradiated tissue 38 . Quantification of data were performed by S.A., N.M.D.D., and R.A.P., who were all blinded to the group assignment.…”

Section: Animals Female Adult 60-day-old Cd-1 Nude Immunocompromisedmentioning

confidence: 99%

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Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis

Shen

Borrelli

Adem

et al. 2020

Sci Rep

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Radiation therapy can result in pathological fibrosis of healthy soft tissue. The iron chelator deferoxamine (DFO) has been shown to improve skin vascularization when injected into radiated tissue prior to fat grafting. Here, we evaluated whether topical DFO administration using a transdermal drug delivery system prior to and immediately following irradiation (IR) can mitigate the chronic effects of radiation damage to the skin. CD-1 nude immunodeficient mice were split into four experimental groups: (1) IR alone (IR only), (2) DFO treatment for two weeks after recovery from IR (DFO post-IR), (3) DFO prophylaxis with treatment through and post-IR (DFO ppx), or (4) no irradiation or DFO (No IR). Immediately following IR, reactive oxygen species and apoptotic markers were significantly decreased and laser doppler analysis revealed significantly improved skin perfusion in mice receiving prophylactic DFO. Six weeks following IR, mice in the DFO post-IR and DFO ppx groups had improved skin perfusion and increased vascularization. DFO-treated groups also had evidence of reduced dermal thickness and collagen fiber network organization akin to non-irradiated skin. Thus, transdermal delivery of DFO improves tissue perfusion and mitigates chronic radiation-induced skin fibrosis, highlighting a potential role for DFO in the treatment of oncological patients.

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Section: Discussionmentioning

confidence: 99%

Section: Animals Female Adult 60-day-old Cd-1 Nude Immunocompromisedmentioning

confidence: 99%

Section: Animals Female Adult 60-day-old Cd-1 Nude Immunocompromisedmentioning

confidence: 99%

Section: Animals Female Adult 60-day-old Cd-1 Nude Immunocompromisedmentioning

confidence: 99%

See 2 more Smart Citations

Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis

Shen

Borrelli

Adem

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

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“…5 Inadequate tissue vascularization is the main cause of low graft survival. 6 Flacco et al 7 demonstrated that deferoxamine preconditioning improves perfusion and fat graft retention. Okyay et al 8 proved that treatment of fat grafts with the selective beta-1-blocker metoprolol resulted in a good quality graft.…”

Section: Introductionmentioning

confidence: 99%

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Yin

et al. 2020

Journal of Plastic, Reconstructive & Aesthetic Surgery

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Background: Cell-assisted lipotransfer (CAL) promotes the survival of fat grafts with high vascular density and improves skin quality by increasing collagen content. However, no study has quantified the changes on the skin surface, and rigorous methodological evaluations are still lacking. Design: Fifty patients were recruited and randomly divided into two groups: an experimental group (n = 25) that underwent a stromal vascular fraction (SVF)-assisted fat graft and a control group (n = 25) that underwent fat graft only. Methods: The SVF cells were counted, tested in terms of viability, and characterized. The volumes of whole faces were determined by using a 3D scanner and Geomagic software preoperation, immediately after surgery, and 6 months postoperation. Facial skin qualities, including spots, wrinkles, texture, pores, UV spots, brown spots, red areas, and porphyrins, were detected by a VISIA skin detector preoperation and 6 months postoperation. A visual analog scale was used for clinical evaluation. Results: The cell pellet contained 1-3 × 10 7 /mL of fresh SVF cells. The cell viability exceeded 98%. The immunophenotyping characteristics and stemness were consistent with the features of adipose-derived stem cells (ADSCs). The survival rate of SVF-enriched fat grafts was significantly higher than that of control grafts: 77.6% ±11.6% versus 56.2% ±9.5% (p < 0.001). The VISIA values of wrinkles (19.3 ± 6.6 versus 10.9 ± 5.5, p < 0.001) and texture (15.8 ± 7.0 versus 10.3 ± 5.0, p < 0.01) were significantly higher in SVF-enriched group than in control group at 6 months postoperation. During long-term follow-up, the majority of patients in both groups were satisfied with the final facial esthetic results.

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Effects of Mesenchymal Stem Cell‐Derived Paracrine Signals and Their Delivery Strategies

Chang

Yan

Yao

et al. 2021

Adv Healthcare Materials

137

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Mesenchymal stem cells (MSCs) have been widely studied as a versatile cell source for tissue regeneration and remodeling due to their potent bioactivity, which includes modulation of inflammation response, macrophage polarization toward proregenerative lineage, promotion of angiogenesis, and reduction in fibrosis. This review focuses on profiling the effects of paracrine signals of MSCs, commonly referred to as the secretome, and highlighting the various engineering approaches to tune the MSC secretome. Recent advances in biomaterials‐based therapeutic strategies for delivery of MSCs and MSC‐derived secretome in the form of extracellular vesicles are discussed, along with their advantages and challenges.

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Deferoxamine Preconditioning of Irradiated Tissue Improves Perfusion and Fat Graft Retention

Abstract: The authors' results demonstrated increased perfusion with deferoxamine treatment, which was also associated with improved fat graft volume retention. Preconditioning with deferoxamine may thus enhance fat graft outcomes for soft-tissue reconstruction following radiation therapy.

Cited by 48 publications

References 39 publications

Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis

Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Effects of Mesenchymal Stem Cell‐Derived Paracrine Signals and Their Delivery Strategies

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