Deficiency in Acellular Cementum and Periodontal Attachment in <i>Bsp</i> Null Mice

Foster, Brian L.; Soenjaya, Yohannes; Nociti, Francisco Humberto; Holm, Erik; Zerfas, Patricia M.; Wimer, Helen F.; Holdsworth, David W.; Aubin, Jane E.; Hunter, G.K.; Goldberg, H. A.; Somerman, Martha J.

doi:10.1177/0022034512469026

Cited by 93 publications

(138 citation statements)

References 34 publications

Supporting

Mentioning

131

Contrasting

Order By: Relevance

“…Preparation and genotyping of Bsp homozygous knock-out (Bsp −/− ) and WT mice were described previously [28]. Mice were maintained on a mixed 129/CD1 background and were fed a standard pelleted mouse diet (2018 Tekland Global 18% protein diet, Harlan Laboratories, USA) and tap water ad libitum.…”

Section: Animal Protocolmentioning

confidence: 99%

Loss of bone sialoprotein leads to impaired endochondral bone development and mineralization

Holm

Aubin

Hunter

et al. 2015

Bone

View full text Add to dashboard Cite

Section: Animal Protocolmentioning

confidence: 99%

Loss of bone sialoprotein leads to impaired endochondral bone development and mineralization

Holm

Aubin

Hunter

et al. 2015

Bone

View full text Add to dashboard Cite

“…Procedures for picrosirius red stain, tartrate-resistant acid phosphatase (TRAP), and immunohistochemistry (IHC) were as previously described (Foster et al, 2013). The Appendix Fig.…”

Section: Histologymentioning

confidence: 99%

Fibromodulin and Biglycan Modulate Periodontium through TGFβ/BMP Signaling

et al. 2014

Self Cite

View full text Add to dashboard Cite

A full understanding of the key regulators controlling periodontal development and homeostasis is necessary for the design of improved periodontal regenerative therapies. Small leucinerich proteoglycans (SLRPs) are extracellular matrix molecules suggested to regulate collagen organization and cell signaling. Mice with double-deficiency of 2 SLRPs, fibromodulin and biglycan (dKO), acquire skeletal abnormalities, but their roles in regulating the periodontium remain undefined and were the focus of our studies. Transmission electron microscopy studies showed abnormal collagen fibrils in the periodontal ligament (PDL) and altered remodeling of alveolar bone in dKO mice. Immunohistochemistry (IHC) revealed increased staining of SLRPs (asporin, lumican, and decorin) and dentin matrix protein-1 (DMP1, a mechanosensory/osteocyte marker), while osteoblast markers, bone sialoprotein and osteopontin, remained unchanged. Disruption of homeostasis was further evidenced by increased expression of receptor-activator of nuclear factor-κB ligand (RANKL) and elevated numbers of osteoclasts, especially noted around the alveolar bone of molars (buccal side) and incisors. Polymerase chain reaction (PCR) array revealed hyperactive transforming growth factors beta/bone morphogenetic protein (TGFβ/BMP) signaling in dKO PDL tissues, which was further confirmed by elevated expression of phosphorylated Smad5 (p-Smad5) by IHC in dKO PDL. These studies highlight the importance of SLRPs in maintaining periodontal homeostasis through regulation of TGFβ/BMP signaling, matrix turnover, and collagen organization.

show abstract

“…A cellular extrinsic fibers cementum (AEFC) is the tissue covering the cervical portion of the tooth root, important for attachment of the periodontal ligament (PDL) to the root surface [25]. In this study, the AEFC was absent on root surface and furcation.…”

Section: Cementummentioning

confidence: 74%