Deficiency of immune interferon production by leukocytes of normal newborns

Bryson, Yvonne J.; Winter, Harland S.; Gard, Sherrie E.; Fischer, Thomas J.; Stiehm, E. Richard

doi:10.1016/0008-8749(80)90150-1

Cited by 162 publications

(60 citation statements)

References 34 publications

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“…Along with the diminished GM-CSF production shown in this report, neonatal T cells have an even greater impairment in the production of interferon-7 (32,40,41) and IL-4 (42) compared with adult T cells. This immaturity in lymphokine production is at least in part selective, inasmuch as IG2 (32,43) and lymphotoxin (33) are produced in normal or increased amounts by neonatal T cells in vitro.…”

Section: Discussionmentioning

confidence: 55%

Decreased Granulocyte-Macrophage Colony-Stimulating Factor Production by Human Neonatal Blood Mononuclear Cells and T Cells

et al. 1992

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Section: Discussionmentioning

confidence: 55%

Decreased Granulocyte-Macrophage Colony-Stimulating Factor Production by Human Neonatal Blood Mononuclear Cells and T Cells

et al. 1992

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“…The serum level of sFctRII as well as that of IgE might be affected by these lymphokines. However, we have little knowledge about the behavior of these lymphokines in allergic and nonallergic children, although the inability of T cells to produce IFN-7 has been noted in neonates (43,44). Advances in the study of these lymphokines, as well as of sFccRII, may provide a solution to this problem.…”

Section: Discussionmentioning

confidence: 99%

Soluble Low Affinity Fc Receptors for IgE in the Serum of Allergic and Nonallergic Children

et al. 1989

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ABSTRACT. IgE-binding factors are thought to have regulatory activity in in vitvo IgE synthesis. To obtain evidence of the participation of IgE-binding factors in in vivo IgE synthesis, the serum level of low affinity Fc receptors for IgE (sFceRI1) (IgE-BFs) was examined in 41 nonallergic children and in 37 allergic children whose serum IgE levels were significantly higher than those of nonallergic children. The serum level of sFctRII showed a marked age-dependent variation. It was highest in infants and then decreased gradually with age. The serum level of sFctRII in allergic children was significantly higher than that of nonallergic children in early childhood (1128.0 f 323.8 vs 777.3 2 227.0 pg/ml, p < 0.01 in infants (

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“…PHA-induced LIF productheir studies of lymphotoxin production in cord blood. We recently reported deficient immune interferon production despite normal classical interferon production, suggesting selective lymphokine abnormalities among newborns (3). Utilizing many of the same supernatants generated during the interferon studies, we measured MIF and LIF activity in cord blood lymphocytes and newborn lymphocytes after activation with PHA or allogeneic lymphocytes in a MLC.…”

Section: Discussionmentioning
confidence: 99%

Deficient Lymphokine Production of Newborn Lymphocytes
Winter
¹
,
Gard
²
,
Fischer
³

et al. 1983
Pediatr Res
19
1
7
0
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SummaryLymphokine production by newborn lymphocytes was assessed by measuring migration inhibition factor (MIF) and leukocyte inhibition factor (LIF) of isolated mononuclear cells from cord blood, 1-7-days-old newborns, and adult controls. Ficoll-Hypaque separated mononuclear cells were stimulated with phytohemagglutinin (PHA) or allogeneic lymphocytes in a mixed leukocyte culture (MLC), and the supernatants were harvested at optimal times for lymphokine assays. Thymidine incorporation into DNA was also assayed to calculate a proliferative index. M I F was assessed by the inhibition of adult mononuclear phagocyte cell migration under agarose; LIF was assessed by polymorphonuclear cell migration under agarose. Although the proliferative responses of cord and newborn cells are equivalent or greater than those of adult controls, the PHA-induced MIF production in cord blood and newborn lymphocytes was only 46% and 12.5% respectively of mean adult levels; MLC-induced MIF production was 44% and 7%. resoectivelv of mean adult levels. PHA-induced LIF productheir studies of lymphotoxin production in cord blood. We recently reported deficient immune interferon production despite normal classical interferon production, suggesting selective lymphokine abnormalities among newborns (3). Utilizing many of the same supernatants generated during the interferon studies, we measured MIF and LIF activity in cord blood lymphocytes and newborn lymphocytes after activation with PHA or allogeneic lymphocytes in a MLC.A new adaptation of the agarose chemotactic assay (10) was used to measure MIF in the supernatant of cultured cells and the Clausen modification of the polymorphonuclear leukocyte migration under agarose assay was used to measure LIF. Timed samples were obtained to determine the kinetics of lymphokine production. We found that after serial dilutions of the supernatants there were significant differences in both MIF and LIF production among newborn, cord, and adult cells. MATERIALS AND METHODS .tion in cord blood was 27% of adult levels. These differences are only appreciated if dilutions of the supernatants are assayed. Simultaneous assay of MIF and LIF production in dilution of supernatants from adult lymphocytes showed higher LIF activity, whereas in cord lymphocytes MIF activity was greater than LIF activity. This further emphasizes the non-identity of MIF and LIF. These results indicate another abnormality of T cellular immunity in newborns not detected by T-cell enumeration or proliferative responses and parallels other defects in specialized T cell function such as cytotoxicity and immune interferon production. Despite normal thymic size and function, normal T cell numbers and subsets, and vigorous proliferative responses to mitogens, cellular immunity in the newborn human is functionally deficient. This is suggested by the newborns' enhanced susceptibility to viral infection (4, 17), diminished delayed cutaneous hypersensitivity reactions (2, 24), and selective abnormalities (when compared to adults) of in vitro mea...

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Deficiency of immune interferon production by leukocytes of normal newborns

Cited by 162 publications

References 34 publications

Decreased Granulocyte-Macrophage Colony-Stimulating Factor Production by Human Neonatal Blood Mononuclear Cells and T Cells

Decreased Granulocyte-Macrophage Colony-Stimulating Factor Production by Human Neonatal Blood Mononuclear Cells and T Cells

Soluble Low Affinity Fc Receptors for IgE in the Serum of Allergic and Nonallergic Children

Deficient Lymphokine Production of Newborn Lymphocytes

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