Déficit hídrico induzido por manitol para seleção de genótipos de tomateiro

Maciel, Gabriel Mascarenhas; Berenguer, Andrea Farneze; Silva, Ernani C. da; Fraga, E. F.; Rocha, Débora Kelli; Rocha, Jaíne Priscila R. da

doi:10.4322/rca2482

Cited by 2 publications

(3 citation statements)

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“…The mannitol concentrations were calculated by the Van't Hoff formula, i.e., Yos = -RTC, where Yos is the osmotic potential (atm), R is the ideal gas constant (8.32 J mol -1 K -1 ), T is temperature (K), and C is the concentration in g L -1 of water (Morales et al, 2015, Maciel et al, 2017 used to obtain each level of osmotic potential. Moreover, 1 MPa = 10 bar, 1 bar = 0.987 atm, and T (K) = 273 + T (ºC).…”

Section: Experiments Onementioning

confidence: 99%

Does the substrate affect the germination of soybean seeds treated with phytosanitary products?

et al. 2020

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The use of an appropriate method that accurately expresses physiological seed quality and minimizes possible phytotoxicity by chemical products in laboratory testing with soybean seeds is relevant. The objective of this work was to evaluate the influence and infer the method adequacy for the representative evaluation of physiological quality of soybean seeds treated with phytosanitary products. Two experiments were carried out in a completely randomized design, with trial 1 in a 9×5 factorial scheme involving nine seed treatments, including fungicides and insecticides, and solutions with five osmotic potentials (0, -0.25, -0.5, -0.75 and -1.0 MPa) for wetting a paper substrate in a germination experiment, with evaluation of the normal seedlings at five and eight days after sowing. The second experiment consisted of a 9×7 factorial, with nine seed treatments and seven methods for germination evaluation at 5 days (between paper - BP, between sand - BS, between paper with water restriction - BPWR, between paper with preconditioning - BPC, sand between paper - SBP and vermiculite between paper - VBP). Treatments with insecticides affect seedling germination and evaluation, with greater phytotoxicity in relation to fungicides. Methods with readily available water led to greater phytotoxicity than methods with low water availability. For soybean germination evaluation of seeds treated with phytosanitary products, the BPC, SBP and VBP methods were optimal.

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Section: Experiments Onementioning

confidence: 99%

Does the substrate affect the germination of soybean seeds treated with phytosanitary products?

et al. 2020

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“…For this, 20 seeds of Seeds were disinfected by immersion in ethanol (70%) for 30 s and transferred to a 5% sodium hypochlorite solution for 10 min, followed by three rinses in autoclaved distilled water. To simulate drought, MS culture media was prepared (Murashige & Skoog, 1962) with 20 g L À1 sucrose, 8 g L À1 agar and supplemented with 22.29 g L À1 of mannitol PA (C 6 H 14 O 6 -P.M. 182.17) to reach the osmotic potential of À.3 MPa (Maciel et al, 2017). The pH of the culture medium was adjusted to 5.8.…”

Section: First Experimental Set: In Vitro Assaymentioning

confidence: 99%

“…The evaluations were performed in a laminar flow chamber, maintaining aseptic conditions. Seeds that did not germinate or seedlings with reduced development due to mannitol were considered susceptible to drought (Maciel et al, 2017).…”

Section: First Experimental Set: In Vitro Assaymentioning

confidence: 99%

Selection for drought tolerance in backcross populations derived from interspecific crosses of Solanum lycopersicum × Solanum pennellii

Henschel,

Batista,

de Souza de Voltare

et al. 2023

Plant Breeding

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Drought strongly limits tomato yield, and the introgression of genes from wild tomatoes is a powerful tool to obtain drought‐tolerant progenies. The aim of this study was to select drought‐tolerant transgressive progeny obtained from interspecific crosses between drought‐susceptible tomatoes (Solanum lycopersicum) × drought‐tolerant wild species (Solanum pennellii) under in vitro and greenhouse conditions. BC1F2 populations were advanced from backcrosses between F1 × Jumbo AG‐592 (cultivar for fresh consumption) and F1 × BRS Tospodoro (cultivar for industrial processing). For this, BC1F2 seeds were germinated in vitro and evaluated for tolerance to drought. Then, eight genotypes from each BC1F2 were selected and submitted to 14 days of drought (0% of water supply) or well‐watered (100% of water supply) in greenhouse conditions and evaluated for growth, water balance and gas exchanges. Using in vitro assays with mannitol‐induced drought proved to be effective for the initial screening of drought‐tolerant BC1F2 plants, while greenhouse experiments showed that drought decreased photosynthesis in all genotypes, but almost all the BC1F2 progenies had greater photosynthetic capacity, water balance and growth than their commercial parents. As a result, we selected six progenies for fresh consumption and six progenies for industrial processing with increased drought tolerance.

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Déficit hídrico induzido por manitol para seleção de genótipos de tomateiro

Cited by 2 publications

References 10 publications

Does the substrate affect the germination of soybean seeds treated with phytosanitary products?

Does the substrate affect the germination of soybean seeds treated with phytosanitary products?

Selection for drought tolerance in backcross populations derived from interspecific crosses of Solanum lycopersicum × Solanum pennellii

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