Defined topologically-complex protein matrices to manipulate cell shape<i>via</i>three-dimensional fiber-like patterns

Moraes, Christopher; Kim, Byoung Choul; Zhu, Xiaoyue; Mills, Kristen; Dixon, Angela R.; Thouless, M.D.; Takayama, Shuichi

doi:10.1039/c4lc00122b

Cited by 24 publications

(19 citation statements)

References 64 publications

(104 reference statements)

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“…The specific alignment of cells and the ECM can affect cell function, physiology, and the differentiation of stem cells [41–43]. Cell sheets with engineered cellular patterns generated on tissue-specific surface topographies can more readily mimic native cell assembly and may accelerate progression to the final tissue-engineered construct.…”

Section: Cell Assemblymentioning

confidence: 99%

Multiscale assembly for tissue engineering and regenerative medicine

Güven¹,

Pu²,

İnci³

et al. 2015

Trends in Biotechnology

166

134

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Our understanding of cell biology and its integration with materials science has led to technological innovations in the bioengineering of tissue-mimicking grafts that can be utilized in clinical and pharmaceutical applications. Bio-engineering of native-like multiscale building blocks provides refined control over the cellular microenvironment, thus enabling functional tissues. In this review, we focus on assembling building blocks from the biomolecular level to the millimeter scale. We also provide an overview of techniques for assembling molecules, cells, spheroids, and microgels and achieving bottom-up tissue engineering. Additionally, we discuss driving mechanisms for self- and guided assembly to create micro-to-macro scale tissue structures.

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Section: Cell Assemblymentioning

confidence: 99%

Multiscale assembly for tissue engineering and regenerative medicine

Güven¹,

Pu²,

İnci³

et al. 2015

Trends in Biotechnology

166

134

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“…Returning to the example of crack‐patterned microbes from Section , Moraes et al created 3D biofilm patterns by spanning 30 µm deep and up to 80 µm wide trenches containing 15 µm wide microcracks . Extracellular matrix proteins from fibroblast and myoblast cells adsorbed on the cracks, eventually spanned the trenches and lead to a complete filling thereof.…”

Section: Guiding Organismsmentioning

confidence: 99%

A Perspective on Bio‐Mediated Material Structuring

et al. 2017

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Bioinspiration, biomorphy, biomimicry, biomimetics, bionics, and biotemplating are terms used to describe the fabrication of materials or, more generally, systems to solve technological problems by abstracting, emulating, using, or transferring structures from biological paradigms. Herein, a brief overview of how the different terminologies are being typically applied is provided. It is proposed that there is a rich field of research that can be expanded by utilizing various novel approaches for the guidance of living organisms for "bio-mediated" material structuring purposes. As examples of contact-based or contact-free guidance, such as substrate patterning, the application of light, magnetic fields, or chemical gradients, potentially interesting methods of creating hierarchically structured monolithic engineering materials, using live patterned biomass, biofilms, or extracellular substances as scaffolds, are presented. The potential advantages of such materials are discussed, and examples of live self-patterning of materials are given.

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“…An alternative approach not involving oxidation consists of incubating PDMS in benzophenone and exposing it to UV to polymerize the acrylic acid on the surface and bind proteins. Figure adapted with permission from [19, 99, 120, 202]. …”

Section: Figurementioning

confidence: 99%

For whom the cells pull: Hydrogel and micropost devices for measuring traction forces

Ribeiro

Denisin

Wilson

et al. 2016

Methods

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While performing several functions, adherent cells deform their surrounding substrate via stable adhesions that connect the intracellular cytoskeleton to the extracellular matrix. The traction forces that deform the substrate are studied in mechanotrasduction because they are affected by the mechanics of the extracellular milieu. We review the development and application of two methods widely used to measure traction forces generated by cells on 2D substrates: i) traction force microscopy with polyacrylamide hydrogels and ii) calculation of traction forces with arrays of deformable microposts. Measuring forces with these methods relies on measuring substrate displacements and converting them into force. We describe approaches to determine force from displacements and elaborate on the necessary experimental conditions for this type of analysis. We emphasize device fabrication, mechanical calibration of substrates and covalent attachment of extracellular matrix proteins to substrates as key features in the design of experiments to measure cell traction forces with polyacrylamide hydrogels or microposts. We also report the challenges and achievements in integrating these methods with platforms for the mechanical stimulation of adherent cells. The approaches described here will enable new studies to understand cell mechanical outputs as a function of mechanical inputs and the understanding of mechanotransduction mechanisms.

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Defined topologically-complex protein matrices to manipulate cell shapeviathree-dimensional fiber-like patterns

Cited by 24 publications

References 64 publications

Multiscale assembly for tissue engineering and regenerative medicine

Multiscale assembly for tissue engineering and regenerative medicine

A Perspective on Bio‐Mediated Material Structuring

For whom the cells pull: Hydrogel and micropost devices for measuring traction forces

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