Defining a global research and policy agenda for betel quid and areca nut

Mehrtash, Hedieh; Duncan, Kalina; Parascandola, Mark; David, Annette M.; Gritz, Ellen R.; Gupta, Prakash C.; Mehrotra, Ravi; Nordin, Amer Siddiq Amer; Pearlman, Paul C.; Warnakulasuriya, Saman; Wen, Chi Pang; Zain, Rosnah Binti; Trimble, Edward L.

doi:10.1016/s1470-2045(17)30460-6

Cited by 161 publications

(123 citation statements)

References 55 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…BQ chewing is dose-dependently associated with the incidence of oral cancer 1,13 and estimates suggest that AN chewing may be responsible for up to 50% of oral cancer in some countries. 14 The BENIT is the first trial aimed at AN cessation in the western Pacific region and was recently implemented in Guam and Saipan 6 to address the disproportionally high extent of AN chewing and the associated high oral cancer rates among minority Pacific Islander populations. This trial is the first of its kind and, therefore, requires measurable indicators to examine its success and efficacy of achieving its overall goal of preventing oral cancers resulting from AN and BQ chewing.…”

Section: Discussionmentioning

confidence: 99%

Areca alkaloids measured from buccal cells using DART‐MS serve as accurate biomarkers for areca nut chewing

Franke

Biggs

Yew

et al. 2019

Drug Testing and Analysis

View full text Add to dashboard Cite

Background Areca nut (AN) chewing is carcinogenic and biomarkers reflecting it are urgently needed to determine the effectiveness of emergent cessation programs. Buccal cells (BCs) may serve as an ideal matrix to measure such biomarkers; however, their utility for this purpose is unknown. Direct analysis in real time−mass spectrometry (DART−MS) is a sensitive technique that analyzes materials in the open air and requires minimal/no sample preparation. We utilized DART−MS to analyze BCs to test the usefulness of this method in measuring areca alkaloids as biomarkers for AN chewing. Methods We applied DART−MS in positive‐ion mode to quantitate over time human BCs: (a) exposed ex vivo to betel quid extracts (BQE) consisting of young AN, Piper betle L. leaf, slaked lime, and tobacco; and (b) obtained from seven chewers before and after BQ chewing. Quantification was performed by normalizing DART−MS alkaloid signal intensities to cholesterol intensities. Results Signals for areca alkaloids arecoline and arecaidine‐guvacoline were detected in BCs exposed ex vivo to BQE up to 7 days (the last day tested) after exposure and in BCs from chewers up to 3 days (the last day tested) post chewing. Discussion The presence of alkaloid signals in BQ‐exposed BCs verified BCs as a valid matrix and DART−MS as a suitable technique to measure biomarkers for AN chewing and provided reliable information on AN chewing timing. Conclusion DART−MS analyses of BCs can be used to accurately determine areca alkaloids as AN chewing biomarkers up to 3 days post chewing and possibly longer.

show abstract

Section: Discussionmentioning

confidence: 99%

Areca alkaloids measured from buccal cells using DART‐MS serve as accurate biomarkers for areca nut chewing

Franke

Biggs

Yew

et al. 2019

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…It is well established that chronic use of chewing tobacco and gutka can be one of potential agent in the prevalence of early onset of oral cancers in Indian sub-continent [1][2][3][4][5][6]. In this paper, we report the clear, reliable and efficient extraction of genotoxic agents in DMSO solvent from chewing tobacco and gutka in an appreciable amount.…”

Section: Resultsmentioning

confidence: 90%

“…In the developing countries of Indian sub-continent, an appreciable portion of populations are habituated to use chewing tobacco and gutka as a part of their lifestyles and to have stimulant and relaxation effects [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…In recent, several clinical and basic scientists proposed views that these consumed chewing tobacco and gutka products contains several carcinogenic chemicals such as proprionitrile, nitrosamines, nicotine, sweeteners, flavoring agents [4][5][6]. However, there is a lack of clear evidence on the genotoxic abilities of these chewing tobacco and gutka.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential DNA damaging effects of genotoxic agents from chewing tobacco and gutka

Kumar¹,

Sharma²

2018

Hematol Med Oncol

View full text Add to dashboard Cite

Background:At the global level and specifically in Indian sub-continent, the use of chewing tobacco and gutka, a type of tobacco products is reported to cause oral submucous fibrosis (OSMF), which can potentially progress to oral cancer. There is no clear view to suggest that both chewing tobacco and gutka may lead to oral carcinogenesis based on chemical ingredients such as betel quid, areca nuts, and slaked lime, sweeteners, carcinogenic compounds and their ability to act as genotoxic agents.

show abstract

“…They are risk factors for both oral and esophageal cancers but unlike tobacco, no political or official restriction to these carcinogens has been documented in these countries. It is thought that further investigations of BQ-and AN-associated mechanisms may be required for the establishment of restriction policies for BQ and AN users [1].…”

Section: Introductionmentioning

confidence: 99%

Mechanistic and compositional studies of the autophagy-inducing areca nut ingredient

Chiu

Liu

Yen

et al. 2019

Preprint

View full text Add to dashboard Cite

Areca nut (AN) is a popular chewing carcinogen worldwide causing a variety of diseases such as oral and esophageal carcinomas. We previously found that the partially purified 30-100 kDa fraction of AN extract (ANE 30-100K) induces autophagy in oral carcinoma OECM-1 cells and some other different types of cells. Since autophagy is known to play important roles in tumor establishment and development, the underlying mechanisms of ANE 30-100K-induced autophagy (AIA) is worthy of further investigation. In this study, we further demonstrated that the cytotoxic concentration of ANE 30-100K induces some typical autophagy hallmarks in esophageal carcinoma (CE81T/VGH) cells in an Atg5-dependent manner. Furthermore, the endocytosis inhibitor (methyl-β-cyclodextrin) and two caveolin shRNAs, as well as two proteasome inhibitors (lactacystin and epoxomicin), were shown to attenuate ANE 30-100K-induced cytotoxicity and LC3-II accumulation significantly in OECM-1 and CE81T/VGH cells. Finally, we also analyzed the carbohydrate compositions of ANE 30-100K by phenol-sulfuric acid method and high performance anion exchange chromatography with pulse amperic detector. The results showed that ANE 30-100K contains about 67% carbohydrate and is composed of fucose (5.938%), arabinose (24.631%), glucosamine (8.066%), galactose (26.820%), glucose (21.388%), and mannose (13.157%). Collectively, these results suggest that caveolin-mediated endocytosis and proteasome are required for AIA and the major components of ANE 30-100K are carbohydrates. This study may have provided new knowledges of the action mechanisms and compositions of ANE 30-100K.

show abstract

Defining a global research and policy agenda for betel quid and areca nut

Cited by 161 publications

References 55 publications

Areca alkaloids measured from buccal cells using DART‐MS serve as accurate biomarkers for areca nut chewing

Areca alkaloids measured from buccal cells using DART‐MS serve as accurate biomarkers for areca nut chewing

Differential DNA damaging effects of genotoxic agents from chewing tobacco and gutka

Mechanistic and compositional studies of the autophagy-inducing areca nut ingredient

Contact Info

Product

Resources

About