2023
DOI: 10.21203/rs.3.rs-2566175/v1
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Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi

Abstract: N-linked glycosylation is a critical post translational modification of eukaryotic proteins. N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions. Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite. In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, F… Show more

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