Defining the roles of PvMDH genes in response to salt stress and detailed characterization of the gene family

Yıldız, Serenay; Okay, Aybüke; Büyük, İlker

doi:10.1007/s13562-021-00690-0

Cited by 6 publications

(5 citation statements)

References 70 publications

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“…The morphological variation caused by salt stress is related to the metabolic process [ 13 , 16 , 43 , 44 ]. Malate dehydrogenase (MDH) is a highly active enzyme in plants, an increasing number of MDH genes have been identified in different species, which play an important role in responding to abiotic stress [ 4 , 5 , 21 , 45 ]. At present, only two members of the rice MDH family have been reported [ 9 , 12 ], which are related to rice development and salt tolerance, but we still know little about the functions of other members of the MDH family in the model plant for rice.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we identified 12 MDH genes in the rice genome, which is less than the number of genes identified in diploid G. raimondii (13), tetraploid G. hirsutum (25) [ 22 ] and P. trichocarpa (16) [ 21 ], which is more than the number identified in diploid P. vulgaris (8) [ 45 ]. All OsMDH genes have different subcellular localizations (cytoplasmic, endoplasmic reticulum, and nuclear), which may result in differences in function.…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Identification of MDH Family Genes and Their Association with Salt Tolerance in Rice

Zhang

Wang

Sun

et al. 2022

Plants

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Malate dehydrogenase (MDH) is widely present in nature and regulates plant growth and development, as well as playing essential roles, especially in abiotic stress responses. Nevertheless, there is no comprehensive knowledge to date on MDH family members in rice. In this study, a total of 12 MDH members in rice were identified through genome-wide analysis and divided into three groups on the basis of their phylogenetic relationship and protein-conserved motifs. Evolutionary analysis showed that MDH proteins from rice, maize and wheat shared a close phylogenetic relationship, and the MDH family was conserved in the long-term process of domestication. We identified two segmental duplication events involving four genes, which could be the major force driving the expansion of the OsMDH family. The expression profile, cis-regulatory elements and qRT-PCR results of these genes revealed that a few OsMDH showed high tissue specificity, almost all of which had stress response elements in the promoter region, and ten MDH members were significantly induced by salt stress. Through gene-based association analysis, we found a significant correlation between salt tolerance at the seedling stage and the genetic variation of OsMDH8.1 and OsMDH12.1. Additionally, we found that the polymorphism in the promoter region of OsMDH8.1 might be related to the salt tolerance of rice. This study aimed to provide valuable information on the functional study of the rice MDH gene family related to salt stress response and revealed that OsMDH8.1 might be an important gene for the cultivar improvement of salt tolerance in rice.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of MDH Family Genes and Their Association with Salt Tolerance in Rice

Zhang

Wang

Sun

et al. 2022

Plants

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“…The expression of PtMDH2 in poplar was signi cantly upregulated in response to salt stress [23]. Similarly, the RNAseq and qRT-PCR results revealed that PvMDH-3, PvMDH-6, and PvMDH-8 play and important role in response to salt stress in common bean [24]. Although MDH family genes hold promise as candidate genes for abiotic stress tolerance, many of their functions in other plants remain unknown and require further veri cation through subsequent experiments.…”

Section: Introductionmentioning

confidence: 89%

Functional characterization of malate dehydrogenase gene, HcMDH1 in enhancing abiotic stress tolerance in kenaf (Hibiscus cannabinus L.)

Luo,

Li,

Mubeen

et al. 2024

Preprint

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Drought and salt stress are two important environmental factors that significantly restrict plant growth and production. Malate dehydrogenase is essential to life as it is engaged in numerous physiological processes in cells, particularly those related to abiotic stress reactions. However, a complete understanding on MDH family members in kenaf is not clear yet. In this study, subcellular localization analysis and a yeast transcriptional activation assay revealed that HcMDH1 was localized in chloroplasts but had no transcriptional activation activity. When exposed to salt or drought, yeast cells that express the HcMDH1 gene have a greater survival rate. Overexpression of HcMDH1 in Arabidopsisincreased seed germination rate and root growth when transgenic lines were exposed to varying concentrations of mannitol and NaCl. Subsequent physiological studies revealed that transgenic lines had higher concentrations of soluble carbohydrates, proline, and chlorophyll and lower concentrations of malondialdehyde (MDA) and reactive oxygen species (ROS). Furthermore, inhibiting HcMDH1 in kenaf using virus-induced gene silencing (VIGS) decreased salt and drought tolerance due to elevated ROS and MDA levels. In these silenced lines, the expression of six essential genes engaged in stress-resistance and photosynthesis, namely HcGAPDH, HcGLYK, HcFBA, HcFBPase, HcPGA, and HcLSD, is significantly altered under salt and drought stress. In summary, HcMDH1 is a positive regulator of salt and drought tolerance in kenaf, which may have implications for transgenic breeding.

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“…RNAseq analysis of Pvul-EIL genes was performed using SRA data developed by Hiz et al, (2014) (Büyük et al, 2019;Büyük et al, 2021c;Hiz et al, 2014;Yıldız et al, 2021). As a result, when compared to the control, certain genes were expressed at lower levels, and others at higher levels under salt and drought stress conditions (Figure 7).…”

Section: Responses Of Pvul-eil Genes To Salt and Drought Stress Throu...mentioning

confidence: 99%

EIN3/EIL (Ethylene Insensitive3 / Ethylene Insensitive3 Like) Protein Family in Phaseolus vulgaris: Identification, Evolution and Expression Analysis within the Genome

Akbulut

Şafak

Okay

et al. 2023

Turkish JAF Sci.Tech.

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Ethylene insensitive-3 (EIN3) / Ethylene insensitive-3-like (EIL) protein family is a small family of transcription factors specific to plants that play role in plant growth and development under various environmental conditions. In this study, various bioinformatics approaches were used to make an in-depth identification of the EIN3/EIL family at both the gene and protein levels. So, 11 Pvul-EIL genes were identified and their approximate locations were determined. Various biochemical and physicochemical properties of EIL proteins in Phaseolus vulgaris have been described. It was determined that Pvul-EIL proteins had a length of 447-651 amino acids and a molecular weight of 51.08-70.68 kDa. All duplications occurring in the Pvul-EIL genome were segmental type. It was observed that conserved motif, gene structure and phylogeny analyses all yielded similar results. For instance, it has been understood that genes with same motif type and number have similar gene structures and were located under the same branch in the phylogenetic tree. Pvul-EIL protein homology modeling showed that DNA binding properties and protein structure were similar to Arabidopsis EIN3. According to cis-element analysis, Pvul-EIL genes are engaged in a wide range of functions, including tissue-specific, stress, and hormone-sensitive expression. Additionally, RNAseq data was used to perform a comparative expression analysis of EIL genes. Various Pvul-EIL gene expression levels were detected under salt and drought stress. This is the first study to check the gene expression levels in P. vulgaris using in-silico detection and characterization of EIL genes. Therefore, obtained results can form the basis for future studies.

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Defining the roles of PvMDH genes in response to salt stress and detailed characterization of the gene family

Cited by 6 publications

References 70 publications

Genome-Wide Identification of MDH Family Genes and Their Association with Salt Tolerance in Rice

Genome-Wide Identification of MDH Family Genes and Their Association with Salt Tolerance in Rice

Functional characterization of malate dehydrogenase gene, HcMDH1 in enhancing abiotic stress tolerance in kenaf (Hibiscus cannabinus L.)

EIN3/EIL (Ethylene Insensitive3 / Ethylene Insensitive3 Like) Protein Family in Phaseolus vulgaris: Identification, Evolution and Expression Analysis within the Genome

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