Defining the roles of the hydrogenase 3 and 4 subunits in hydrogen production during glucose fermentation: A new model of a H2-producing hydrogenase complex

Petrosyan, Hripsime; Vanyan, Liana; Trchоunian, Armen; Trchounian, Karen

doi:10.1016/j.ijhydene.2019.09.204

Cited by 25 publications

(15 citation statements)

References 54 publications

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“…The same behaviour, thug in a different concentration range, has been also reported for the photoreforming of glycerol (maximum H2 productivity at 20 vol% substrate concentration) [48]. It should be also mentioned that increasing glucose concentration also showed detrimental in a completely different route to H2, such as the use of hydrogenase with E. coli [49,50].…”

Section: Effect Of Substrate Concentration and Typesupporting

confidence: 72%

Design of efficient photocatalytic processes for the production of hydrogen from biomass derived substrates

Ramis

Bahadori

Rossetti

2021

International Journal of Hydrogen Energy

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The photoreforming of glucose has been studied over TiO2 photocatalyst with different photoreactors, focusing on the effect of the reaction conditions: temperature, pressure, catalyst and substrate concentration. The effect of pressure was particularly significant, decreasing hydrogen evolution rate, but improving the conversion of the substrate. Furthermore, pressure moderately higher than ambient allowed to operate at high temperature (80°C), boosting hydrogen productivity. Most experiments were carried out on glucose photoreforming, but, for the first time, the photoconversion of levulinic acid was investigated, as an interesting product of biomass hydrolysis under harsh conditions.

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Section: Effect Of Substrate Concentration and Typesupporting

confidence: 72%

Design of efficient photocatalytic processes for the production of hydrogen from biomass derived substrates

Ramis

Bahadori

Rossetti

2021

International Journal of Hydrogen Energy

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“…However, in the present study, the interpretation of the data through a Langmuir-type isotherm does not fit the experimental results obtained, but the existence of an initial optimal glucose concentration is evident. In fact, it was reported that the saturation of the catalysts surface can limit the various reaction steps that lead to the conversion to hydrogen of organic compounds present in the aqueous medium [64][65][66]. The same behavior was also reported for the photoreforming of glycerol [67,68].…”

Section: Influence Of Initial Glucose Concentrationsupporting

confidence: 58%

LaFeO3 Modified with Ni for Hydrogen Evolution via Photocatalytic Glucose Reforming in Liquid Phase

et al. 2021

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In this work, the optimization of Ni amount on LaFeO3 photocatalyst was studied in the photocatalytic molecular hydrogen production from glucose aqueous solution under UV light irradiation. LaFeO3 was synthesized via solution combustion synthesis and different amount of Ni were dispersed on LaFeO3 surface through deposition method in aqueous solution and using NaBH4 as reducing agent. The prepared samples were characterized with different techniques: Raman spectroscopy, UltraViolet-Visible Diffuse Reflectance Spettroscopy (UV–Vis-DRS), X-ray Diffraction (XRD), X-ray Photoelectron Spectroscopy (XPS), X-ray Fluorescence (XRF), Transmission Electron microscopy (TEM), and Scanning Electron microscopy (SEM) analyses. For all the investigated photocatalysts, the presence of Ni on perovskite surface resulted in a better activity compared to pure LaFeO3. In particular, it is possible to identify an optimal amount of Ni for which it is possible to obtain the best hydrogen production. Specifically, the results showed that the optimal Ni amount was equal to nominal 0.12 wt% (0.12Ni/LaFeO3), for which the photocatalytic H2 production was equal to 2574 μmol/L after 4 h of UV irradiation. The influence of different of photocatalyst dosage and initial glucose concentration was also evaluated. The results of the optimization of operating parameters indicated that the highest molecular hydrogen production was achieved on 0.12Ni/LaFeO3 sample with 1.5 g/L of catalyst dosage and 1000 ppm initial glucose concentration. To determine the reactive species that play the most significant role in the photocatalytic hydrogen production, photocatalytic tests in the presence of different radical scavengers were performed. The results showed that •OH radical plays a significant role in the photocatalytic conversion of glucose in H2. Moreover, photocatalytic tests carried out with D2O instead of H2O evidenced the role of water molecules in the photocatalytic production of molecular hydrogen in glucose aqueous solution.

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“…Despite the similarity of the membrane arm of FHL-1 and the Complex I (Schwarz et al, 2018), it is has remained unclear if the E.coli enzyme is capable of translocating ions during disproportionation of formate. In fact, this has been a matter of debate for many years (Bagramyan et al, 2003;McDowall et al, 2014McDowall et al, , 2015Trchounian and Sawers, 2014;Pinske and Sargent, 2016;Petrosyan et al, 2019;Trchounian and Trchounian, 2019) and to-date, no studies convincingly established a contribution to proton motive force generation by FHL-1. The growth defects observed here as the cells are forced to carry out HDCR, however, may be additional circumstantial evidence that the forward reaction contributes to critical energy metabolism.…”

Section: Effect Of Elevated Pressure On Cell Growth and Formate Produmentioning

confidence: 99%

HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases

Roger

Reed

Sargent

2021

Preprint

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ABSRACTEscherichia coli is gram-negative bacterium that is a workhorse of the biotechnology industry. The organism has a flexible metabolism and can perform a mixed-acid fermentation under anaerobic conditions. Under these conditions E. coli synthesises a formate hydrogenlyase isoenzyme (FHL-1) that can generate molecular hydrogen and carbon dioxide from formic acid. The reverse reaction is hydrogen-dependent carbon dioxide reduction (HDCR), which has exciting possibilities in bio-based carbon capture and storage if it can be harnessed. In this study, an E. coli host strain was optimised for the production of formate from H2 and CO2 during bacterial growth in a pressurised batch bioreactor. A host strain was engineered that constitutively produced the FHL-1 enzyme and incorporation of tungsten in to the enzyme, in place of molybdenum, helped poise the reaction in the HDCR direction. The engineered E. coli strain showed an ability to grow under fermentative conditions while simultaneously producing formate from gaseous H2 and CO2 supplied in the bioreactor. However, while a sustained pressure of 10 bar N2 had no adverse effect on cell growth, when the culture was placed at or above 4 bar pressure of a H2:CO2 mixture then a clear growth deficiency was observed. Taken together, this work demonstrates that growing cells can be harnessed to hydrogenate carbon dioxide and provides fresh evidence that the FHL-1 enzyme may be intimately linked with bacterial energy metabolism.

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Defining the roles of the hydrogenase 3 and 4 subunits in hydrogen production during glucose fermentation: A new model of a H2-producing hydrogenase complex

Cited by 25 publications

References 54 publications

Design of efficient photocatalytic processes for the production of hydrogen from biomass derived substrates

Design of efficient photocatalytic processes for the production of hydrogen from biomass derived substrates

LaFeO3 Modified with Ni for Hydrogen Evolution via Photocatalytic Glucose Reforming in Liquid Phase

HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases

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Defining the roles of the hydrogenase 3 and 4 subunits in hydrogen production during glucose fermentation: A new model of a H2-producing hydrogenase complex

Cited by 25 publications

References 54 publications

Design of efficient photocatalytic processes for the production of hydrogen from biomass derived substrates

Design of efficient photocatalytic processes for the production of hydrogen from biomass derived substrates

LaFeO3 Modified with Ni for Hydrogen Evolution via Photocatalytic Glucose Reforming in Liquid Phase

HarnessingEscherichia colifor bio-based production of formate under pressurized H2and CO2gases

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HarnessingEscherichia colifor bio-based production of formate under pressurized H₂and CO₂gases