Definitive endoderm derived from human embryonic stem cells highly express the integrin receptors αV and β5

Wong, Jennifer C.; Gao, Steven Y.; Lees, Justin G.; Best, Marie; Wang, Rennian; Tuch, Bernard E.

doi:10.4161/cam.4.1.10627

Cited by 30 publications

(27 citation statements)

References 41 publications

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“…These combinations, designated in Figure 1 as small orange circles, comprise cell surface receptors for all major ECM components. It was also found, as discussed in the previous section, that attachment through either laminin or RGD binding integrins , (Rodin et al, 2010), (Wong et al, 2010), (Braam et al, 2008), (Rowland et al, 2010), (Yoon et al, 2010), (Yap et al, 2011) is sufficient to support undifferentiated proliferation of hECS in serum-free, chemically defined medium. Fig.…”

Section: Biomimetic Surfaces For Stem Cellsmentioning

confidence: 84%

Synthetic Surfaces for Human Embryonic Stem Cell Culture

Fadeev¹,

Melkoumian²

2011

Embryonic Stem Cells - Basic Biology to Bioengineering

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Section: Biomimetic Surfaces For Stem Cellsmentioning

confidence: 84%

Synthetic Surfaces for Human Embryonic Stem Cell Culture

Fadeev¹,

Melkoumian²

2011

Embryonic Stem Cells - Basic Biology to Bioengineering

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“…For example, the integrin a V b 5 , which binds to vitronectin, is highly expressed in definitive endoderm as well as in hPSCs. 53 a V b 5 is required for initial attachment of hiPSCs to a vitronectin substrate, but once attachment is achieved, the proliferation of these cells is not affected by blocking just a V b 5 with antibodies. 54 The inhibition of these cells' proliferation necessitates the blocking of both a V b 5 and b 1 .…”

Section: Potential Mechanisms Of Substrate-cell Interactions In Hpsc mentioning

confidence: 99%

Mechanobiology of Human Pluripotent Stem Cells

Earls

Jin

2013

Tissue Engineering Part B: Reviews

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Human pluripotent stem cells (hPSCs) are self-renewing and have the potential to differentiate into any cell type in the body, making them attractive cell sources for applications in tissue engineering and regenerative medicine. However, in order for hPSCs to find use in the clinic, the mechanisms underlying their self-renewal and lineage commitment must be better understood. Many technologies that have been developed for the maintenance and directed differentiation of hPSCs involve the use of soluble growth factors, but recent studies suggest that other elements of the hPSC microenvironment also influence the growth and differentiation of hPSCs. This includes the influences of cell-cell interactions, substrate mechanics, cellular interactions with extracellular matrix, as well as the nanotopography of the substrate and physical forces such as shear stress, cyclic mechanical strain, and compression. In this review, we highlight the recent progress of this area of research and discuss ways in which the mechanical cues may be incorporated into hPSC culture regimes to improve methods for expanding and differentiating hPSCs.

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“…We have previously shown that definitive endoderm can be successfully derived from single cell preparations of hESCs that were induced to differentiate on laminin or Matrigel coated 2D monolayer cultures [9]. To determine whether definitive endoderm can also be efficiently generated on laminin or Matrigel coated 3D PLGA scaffolds, single cell preparations of hESCs were induced to differentiate in 2D or 3D cultures following a well established differentiation protocol.…”

Section: Differentiation Of Hescs To Definitive Endoderm Can Be Efficmentioning

confidence: 99%

“…Pluripotent hESCs from the cell line HES3 and its green fluorescence protein (GFP) expressing clone Envy (both were generous gifts from Embryonic Stem Cell International via Prof. Ed Stanley of Monash Immunology and Stem Cell Laboratories) were maintained on gamma irradiated human fetal fibroblast feeder layers and cultured as previously described [9].…”

Section: Cell Culturementioning

confidence: 99%

“…Previously, we investigated the adhesion of hESCs on 3D scaffolds made from poly(lactic-co-glycolic acid) (PLGA), an FDA approved polymer, and their interactions with surrounding matrices during differentiation towards the endoderm lineage [7,9]. The present study aimed to explore the feasibility of using single cell preparations of hESCs seeded onto laminin or Matrigel coated 3D PLGA scaffolds to derive definitive endoderm, the first vital stage of endoderm tissue differentiation.…”

Section: Introductionmentioning

confidence: 99%

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The Generation of Definitive Endoderm from Human Embryonic Stem Cells on 3D Biodegradable Poly(lactic-co-glycolic Acid) Scaffolds and its Comparison to those Generated on 2D Monolayer Cultures

Gao

Wong

Lees

et al. 2011

TOSCJ

Self Cite

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Abstract:The generation of insulin producing cells from human embryonic stem cells (hESCs) has shown great promise as a cellular replacement therapy for the treatment of Type 1 Diabetes. Mature functional β-cell surrogates however, have yet to be successfully generated in vivo. One approach to potentially improve current differentiation protocols is the use of 3 dimensional (3D) scaffolds, which has been shown to enhance cellular function and differentiation potential. The present study aimed to explore the feasibility of using single cell preparations of pluripotent hESCs seeded onto laminin or Matrigel coated 3D poly(lactic-co-glycolic) acid (PLGA) scaffolds to derive definitive endoderm, the first vital stage of endoderm tissue differentiation. Our results demonstrated that hESCs which were induced to differentiate on laminin or Matrigel coated 3D scaffolds can be successfully coaxed to differentiate into definitive endoderm. The cells that were cultured on laminin or Matrigel coated 3D scaffolds expressed significantly higher levels of the key endoderm transcription factors SOX17 and FOXA2 in comparison to those differentiated on 2D monolayers. On Matrigel coated 3D scaffolds, the differentiated cells expressed lower levels of the endoderm surface marker CXCR4 and anterior endoderm marker CER in comparison to its monolayer counterpart. Together, the results of this study demonstrated the positive effect of 3D cultures on endoderm commitment from hESCs over traditional monolayer cultures. Furthermore, the definitive endoderm produced on Matrigel coated scaffolds may have a more posterior phenotype in comparison to those derived from monolayers. This may have an effect on later stages of pancreatic differentiation and warrants further detailed investigations.

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Definitive endoderm derived from human embryonic stem cells highly express the integrin receptors αV and β5

Cited by 30 publications

References 41 publications

Synthetic Surfaces for Human Embryonic Stem Cell Culture

Synthetic Surfaces for Human Embryonic Stem Cell Culture

Mechanobiology of Human Pluripotent Stem Cells

The Generation of Definitive Endoderm from Human Embryonic Stem Cells on 3D Biodegradable Poly(lactic-co-glycolic Acid) Scaffolds and its Comparison to those Generated on 2D Monolayer Cultures

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