Defluorination of Monofluoroacetate by Bacteria

Reaction Mechanism of Fluoroacetate Dehalogenase from Moraxella sp. B

“…However, we found that the wild-type enzyme is not affected by toluene when suspended in a reaction mixture at a ratio of 10% (v/v). The reaction mixture containing 10 mol of Tris sulfate (pH 9.5), 0.75 mol of sodium hydroxide, the wild-type or the H272N mutant enzyme (1 mg each), and 10 l of toluene solution containing [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]chloroacetic acid in a final volume of 100 l was incubated at 30°C for 2 h. The reaction mixture was dialyzed against 50 mM potassium phosphate (pH 7.5) for 14 h and then applied to a C 18 reverse phase HPLC column. The elution was carried out with 0.05% trifluoroacetic acid for 5 min, followed by a linear gradient of 0 -80% acetonitrile in 0.05% trifluoroacetic acid as described above.…”

Purification, Crystallization and Properties of Haloacetate Halidohydrolase fromPseudomonasSpecies

“…One type of the enzyme acts well on fluoroacetate but the other does not. The former type enzymes have been studied and partially purified by Goldmanl) and Tonomura et al 2 ) However little is known about their proteinic properties. In the previous paper 3 ) we reported that both types ofhalidohydrolase, H-l and H-2, are present in an isolated strain of Moraxella and their genes are coded on plasmidpUOl.…”

Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances

“…Fluoroacetate dehalogenase catalyzes the hydrolytic cleavage of carbon fluoride in fluoroacetate, yielding glycolate and fluoride. These enzymes have been isolated in phylogenetically diverse bacteria, such as Pseudomonas ( 60 – 63 ), Delftia acidovorans (formerly Moraxella ) ( 64 , 65 ), Burkholderia ( 56 ), and Rhodopseudomonas ( 66 ). Some fluoroacetate dehalogenases, e.g., Fac-DEX H-1 (H-1), Fac-DEX FA1 (FA1), and RPA1163, have undergone comprehensive characterization.…”