Defne Yaprağından (Laurus Nobilis L.) Fenolik Bileşiklerin Ekstraksiyonunda Farklı Yöntemlerin Karşılaştırılması

Abstract

In this study, the microwave-assisted and enzyme-assisted extraction efficiency were compared to solvent extraction. The extraction efficiencies were evaluated in bay leaf extract in terms of phenolic content and antioxidant capacity. The total phenolic content (mg GAE/g) of the extracts from three different extraction methods as a solvent, enzyme-assisted, and microwave-assisted extraction was found 23.29±0.02, 32.45±0.02, and 30.49±0.02, respectively. The highest value for the total phenolic content was found from the enzyme-assisted extraction. DPPH radical scavenging capacity (%) of the extracts from three different extraction methods was found at 36.91%±0.05, 50.72%±0.27, and 41.51%±0.09, respectively. Like the total phenolic content, the highest value for the DPPH radical scavenging capacity was found from the enzyme-assisted extraction. In addition, total dry matter, total ash, total protein, ascorbic acid, and total chlorophyll content of the bay leaf were analyzed.

Development of thermoplastic starch/poly(butylene adipate‐co‐terephthalate) biobased active packaging films and potential usage in the food industry

“…The absorbance was measured at 760 nm by a spectrophotometer (UV–vis., Peak Instruments, C‐7200, Houston, ABD). The results were expressed in mg GAE/g film 18,19 …”

“…The results were expressed in mg GAE/g film. 18,19 Total antioxidant activity The total antioxidant activity (AA%) of film extracts was assessed by DPPH free radical assay. The specimens were reacted with the DPPH radical in an ethanol solution.…”

“…The result for the scavenging rate was also compared with calibration curves and expressed as Trolox equivalent per unit mass of film (μmol Trolox equivalent/g film). 18,19 AA% ¼ 100 À Abs sample À Abs blank À Á Â 100…”

Aromatik bitki ekstraktları ile zenginleştirilmiş fonksiyonel elma suyu üretimi

“…Örnekler 50 º C su banyosunda 5 dk bekletilip akan suyun altında oda sıcaklığına (25 º C) getirildikten sonra 760 nm'de absorbans değerleri okunmuştur. Örneklerin fenolik madde miktarı mg GAE/100 mL olarak ifade edilmiştir (Akyüz et al, 2022;Metiner & Ersus, 2023).…”

“…Ekstrakt (0,5 mL), etanol (3 mL) ve DPPH (0,3 mL) ile elde edilen karışımın 517 nm'deki absorbans değeri kör (etanol + ekstrakt) ve kontrol (etanol + DPPH) numunesine karşılık spektrofotometre ile (Peak Instruments, C-7200, Houston, ABD) belirlenmiştir. Toplam antioksidan kapasite yüzdesi (%AK) Denklem 1 kullanılarak hesaplanmıştır ve % olarak ifade edilmiştir (Akyüz et al, 2022;Tekin et al, 2023).…”