Soybean tolerance to defoliation at the beginning of pod formation as affected by plant density 1Soybean tolerance to defoliation may be affected by population density, as the plant population interferes with the crop leaf area index . This study aimed to evaluate the effect of defoliation at the beginning of pod formation on the agronomic performance of soybean at different plant densities. The experiment was conducted under field conditions in Campos Novos, SC, Brazil, during 2016/2017 and 2017/2018 growing season. The experimental design was a randomized block in a split-plot arrangement. The main plot consisted of three densities (100,000, 300,000, and 500,000 plants ha -1 ), whereas the subplots consisted of the cultivar NA 5909 RG submitted to five defoliation levels (0, 16.6, 33.3, 50, and 66.6%). Grain yield ranged from 4,219 to 5,356 kg ha -1 in the 2016/2017 growing season and 3,732 to 5,186 kg ha -1 in the 2017/2018 growing season. Plant density did not interfere with the grain yield response to defoliation performed at the beginning of pod formation. The 16.6% defoliation increase grain yield by 7.5 and 5.6% relative to the control in 2016/ 2017 and 2017/2018 growing seasons, respectively. The soybean tolerate defoliation of up to 33.3% at R3 with no significant decrease in grain yield, regardless of the plant density.