Deformability of mouse erythrocytes in different diluents measured using optical tweezers

Shao, Meng; Liu, Rui; Li, Changxu; Sun, Yue; Zhong, Zhensheng; Lu, Fengya; Zhou, Jinhua; Zhong, Min-Cheng

doi:10.1039/d3sm00923h

Soft Matter

2023

DOI: 10.1039/d3sm00923h

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Deformability of mouse erythrocytes in different diluents measured using optical tweezers

Meng Shao,

Rui Liu,

Changxu Li

et al.

Abstract: Quantitative evaluation of the impact of different diluents on the elasticity characteristics of mouse erythrocytes was conducted using optical tweezers. The results show that, although the erythrocytes remained intact in the selected diluents, their deformability changed in different diluents.

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2024

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Cited by 3 publications

References 49 publications

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Sperm quality analyzer: A portable LED array microscope with dark‐field imaging

Shao,

Li,

et al. 2024

Bioengineering & Transla Med

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Sperm quality analysis plays an important role in diagnosing infertility, which is widely implemented by computer‐assisted sperm analysis (CASA) of sperm‐swimming imaging from commercial phase‐contrast microscopy. A well‐equipped microscope comes with a high cost, increasing the burden of assessment, and it also occupies a large volume. For point‐of‐care testing (POCT) of sperm quality, these factors are confronted with the challenges of low‐cost and portable instruments. In this study, an encoded light‐emitting diode (LED) array illumination is employed to achieve a portable microscope with multicontrast imaging for sperm quality analysis. This microscopy has dimensions of 16.5 × 14.0 × 25.0 cm, and its dark‐field (DF) imaging provides high‐contrast sperm image data which is suitable for CASA. According to DF imaging, we developed a software of LabCASA, which can used to assess the motility characteristics of sperm. Compared with TrackMate, the difference in motility parameters from our software was less than 10% in the coefficient of variation (CV). The sperm motility parameters vary with the chamber temperature, which further confirms the reliability of our system with DF imaging. The DF imaging provides strong robustness for tracking sperm's motion under different microscopes. For assessment of the motility parameters, our system can work at a lower cost with a plastic structure. This system with DF imaging is suitable for portable POCT of sperm quality analysis, which is highly cost‐effective in resource‐constrained circumstances.

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Sperm quality analyzer: A portable LED array microscope with dark‐field imaging

Shao,

Li,

et al. 2024

Bioengineering & Transla Med

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Optofluidic-based deformability cytometry: Characterizing erythrocyte elasticity and assessing storage quality

Mao,

Li,

Liu

et al. 2024

Sensors and Actuators A: Physical

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Optothermal trapping of microparticles near an absorbing reflective film with an annular beam

Chen,

Jia,

Meng

et al. 2024

Review of Scientific Instruments

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Optothermal manipulation technologies trap and manipulate microparticles under a light-controlled temperature gradient. In this paper, we demonstrate the possibilities of optothermal trap, which is generated by an annular beam irradiating on an absorbing reflective film to capture the microparticles. The particles are subjected to thermal and optical gradient forces. First, we investigate the particle trapping near a non-absorbing reflective surface to understand the action of the optical force of annular beam. The results show that the optical force cannot trap the particles near a reflective surface. Second, an annular beam is generated by Gaussian beam shaping with the aid of axicons, which is then used to irradiate and heat a gold film to create an optothermal trap. The induced thermal gradient and thermal convection can bind the particles in the center of the annular spot. The trapping stiffness of the optothermal trap is 8.1 ± 2.9 fN/μm at a laser power of 100 mW.

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Deformability of mouse erythrocytes in different diluents measured using optical tweezers

Cited by 3 publications

References 49 publications

Sperm quality analyzer: A portable LED array microscope with dark‐field imaging

Sperm quality analyzer: A portable LED array microscope with dark‐field imaging

Optofluidic-based deformability cytometry: Characterizing erythrocyte elasticity and assessing storage quality

Optothermal trapping of microparticles near an absorbing reflective film with an annular beam

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