Deformable mirror-based photoacoustic remote sensing (PARS) microscopy for depth scanning

Mukhangaliyeva, Lyazzat; Kocer, Samed; Warren, Alkris; Bell, Kevan; Boktor, Marian; Yavuz, Mustafa; Abdullrahman, Eihab; Reza, Parsin Haji

doi:10.1364/boe.471770

Cited by 10 publications

(5 citation statements)

References 24 publications

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“…Non-contact imaging developed in Refs. 63 and 59 helps prevent contamination and damage to delicate samples. Lee et al.…”

Section: Pa Plus Advanced Laser Sourcesmentioning

confidence: 99%

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Photoacoustic imaging plus X: a review

Jiang,

Zhu,

Tong

et al. 2023

J. Biomed. Opt.

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. Significance Photoacoustic (PA) imaging (PAI) represents an emerging modality within the realm of biomedical imaging technology. It seamlessly blends the wealth of optical contrast with the remarkable depth of penetration offered by ultrasound. These distinctive features of PAI hold tremendous potential for various applications, including early cancer detection, functional imaging, hybrid imaging, monitoring ablation therapy, and providing guidance during surgical procedures. The synergy between PAI and other cutting-edge technologies not only enhances its capabilities but also propels it toward broader clinical applicability. Aim The integration of PAI with advanced technology for PA signal detection, signal processing, image reconstruction, hybrid imaging, and clinical applications has significantly bolstered the capabilities of PAI. This review endeavor contributes to a deeper comprehension of how the synergy between PAI and other advanced technologies can lead to improved applications. Approach An examination of the evolving research frontiers in PAI, integrated with other advanced technologies, reveals six key categories named “PAI plus X.” These categories encompass a range of topics, including but not limited to PAI plus treatment, PAI plus circuits design, PAI plus accurate positioning system, PAI plus fast scanning systems, PAI plus ultrasound sensors, PAI plus advanced laser sources, PAI plus deep learning, and PAI plus other imaging modalities. Results After conducting a comprehensive review of the existing literature and research on PAI integrated with other technologies, various proposals have emerged to advance the development of PAI plus X. These proposals aim to enhance system hardware, improve imaging quality, and address clinical challenges effectively. Conclusions The progression of innovative and sophisticated approaches within each category of PAI plus X is positioned to drive significant advancements in both the development of PAI technology and its clinical applications. Furthermore, PAI not only has the potential to integrate with the above-mentioned technologies but also to broaden its applications even further.

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“…Non-contact imaging developed in Refs. 63 and 59 helps prevent contamination and damage to delicate samples. Lee et al.…”

Section: Pa Plus Advanced Laser Sourcesmentioning

confidence: 99%

“…Multispectral imaging allows for better differentiation of lipid, hemoglobin, and other absorbers. Ren et al, 62 Mukhangaliyeva et al, 63 and Liang et al 59 showcased handheld, non-contact, and other systems tailored for clinical use. The handheld probe in Ref.…”

Section: Enabling More Clinical Applicationsmentioning

confidence: 99%

Photoacoustic imaging plus X: a review

Jiang,

Zhu,

Tong

et al. 2023

J. Biomed. Opt.

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“…Photoacoustic remote sensing was first used for label-free nuclear imaging in unstained tissues by Haven et al [45] and Abassi et al [46] in 2019. It has also been used to image a variety of biomolecules label-free including hemoglobin [47]- [51], cytochromes [52], [53], DNA/RNA and lipids [54], [55]. More recently, Ecclestone et al [56] presented the next generation method, now called photon absorption remote sensing (PARS), capable of simultaneously capturing scattering, radiative (autofluorescence) and non-radiative relaxation contrasts.…”

Section: Introductionmentioning

confidence: 99%

Automated Whole Slide Imaging for Label-Free Histology Using Photon Absorption Remote Sensing Microscopy

Tweel,

Ecclestone,

Boktor

et al. 2024

IEEE Trans. Biomed. Eng.

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Pathologists rely on histochemical stains to impart contrast in thin translucent tissue samples, revealing tissue features necessary for identifying pathological conditions. However, the chemical labeling process is destructive and often irreversible or challenging to undo, imposing practical limits on the number of stains that can be applied to the same tissue section. Here we present an automated label-free whole slide scanner using a PARS microscope designed for imaging thin, transmissible samples. Methods: Peak SNR and in-focus acquisitions are achieved across entire tissue sections using the scattering signal from the PARS detection beam to measure the optimal focal plane. Whole slide images (WSI) are seamlessly stitched together using a custom contrast leveling algorithm. Identical tissue sections are subsequently H&E stained and brightfield imaged. The one-to-one WSIs from both modalities are visually and quantitatively compared. Results: PARS WSIs are presented at standard 40x magnification in malignant human breast and skin samples. We show correspondence of subcellular diagnostic details in both PARS and H&E WSIs and demonstrate virtual H&E staining of an entire PARS WSI. The one-to-one WSI from both modalities show quantitative similarity in nuclear features and structural information. Conclusion: PARS WSIs are compatible with existing digital pathology tools, and samples remain suitable for histochemical, immunohistochemical, and other staining techniques. Significance: This work is a critical advance for integrating label-free optical methods into standard histopathology workflows.

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“…The optical emissions from the radiative relaxation are broadly captured while the non-radiative contrast is measured as a percentage modulation in the back or forward scattering intensity of a secondary probe beam 16 . Depending on the excitation wavelength, PARS can provide sensitivity to a variety of chromophores including hemoglobin [17][18][19] , cytochromes 20,21 , DNA, collagen, elastin, and lipids 16,22,23 . Furthermore, by simultaneously capturing both absorption fractions, PARS provides additional contrast such as the quantum efficiency ratio (QER).…”

Section: Introductionmentioning

confidence: 99%

Photon Absorption Remote Sensing Imaging of Breast Needle Core Biopsies is Diagnostically Equivalent to Gold Standard H&E Histologic Assessment

Tweel

Ecclestone

Gaouda

et al. 2023

Preprint

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STRUCTURED AbstractOBJECTIVEPhoton absorption remote sensing (PARS) is a new laser-based microscope technique that permits cellular level resolution of unstained fresh, frozen, and fixed tissues. Our objective was to determine whether PARS could provide image quality sufficient for diagnostic assessment of breast cancer needle core biopsies (NCB).DESIGNWe PARS imaged and virtually H&E stained seven independent unstained formalin fixed paraffin-embedded breast NCB sections. These identical tissue sections were then subsequently stained with standard H&E and digitally scanned. Both the 40x PARS and H&E whole slide images were assessed by seven breast cancer pathologists, masked to the origin of the images. A concordance analysis was performed to quantify the diagnostic performances of standard H&E and PARS virtual H&E.RESULTSThe PARS images were deemed of diagnostic quality and pathologists were unable to distinguish the origin of the images above that expected by chance. The diagnostic concordance on cancer vs. benign was high between PARS and conventional H&E (98% agreement) and there was complete agreement for within PARS images. Similarly, agreement was substantial (kappa > 0.6) for diagnosis of specific cancer subtypes. PARS virtual H&E inter-rater reliability was broadly consistent with the published literature on diagnostic performance of conventional histology NCBs across all tested histologic features.CONCLUSIONS AND RELEVANCEPARS was able to provide images on unstained tissues slides that were diagnostically equivalent to conventional H&E. Due to its ability to non-destructively image fixed and fresh tissues, and the suitability of the PARS output for artificial intelligence assistance in diagnosis, this technology has the potential to improve both the speed and accuracy of breast cancer diagnosis.

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Deformable mirror-based photoacoustic remote sensing (PARS) microscopy for depth scanning

Cited by 10 publications

References 24 publications

Photoacoustic imaging plus X: a review

Photoacoustic imaging plus X: a review

Automated Whole Slide Imaging for Label-Free Histology Using Photon Absorption Remote Sensing Microscopy

Photon Absorption Remote Sensing Imaging of Breast Needle Core Biopsies is Diagnostically Equivalent to Gold Standard H&E Histologic Assessment

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