Deformation of single cells - optical two-beam traps and more

Nielsen, Kirstine Sandager; Rungling, Tony B.; Dziegiel, Morten Hanefeld; Marie, Rodolphe; Berg-Sørensen, Kirstine

doi:10.1117/12.2513407

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…Works intracellularly Relies on differences in refractive indices (e.g. DNA-rich parts like the nucleus) The application of optical tweezers for the handling of single cells and for diagnostics on the single cell level has recently been discussed a lot [138,141,154,155]. However, it can as yet not be used as the only technique for any kind of diagnostics, and is either combined with other tests to create assays [63,66], or needs massive automation and robotics [156] or artificial intelligence to arrive at a diagnosis [156,157].…”

Section: Contact-free Micromanipulation Needs Electricity and Lasers mentioning

confidence: 99%

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Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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In the last three decades, microfluidics and its applications have been on an exponential rise, including approaches to isolate rare cells and diagnose diseases on the single-cell level. The techniques mentioned herein have already had significant impacts in our lives, from in-the-field diagnosis of disease and parasitic infections, through home fertility tests, to uncovering the interactions between SARS-CoV-2 and their host cells. This review gives an overview of the field in general and the most notable developments of the last five years, in three parts: 1. What can we detect? 2. Which detection technologies are used in which setting? 3. How do these techniques work? Finally, this review discusses potentials, shortfalls, and an outlook on future developments, especially in respect to the funding landscape and the field-application of these chips.

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Section: Contact-free Micromanipulation Needs Electricity and Lasers mentioning

confidence: 99%

“…For alternative setups of optical traps, it is also possible to use two counter-propagating beams [140,141], or two parallel lasers to generate interference to trap particles [142], or two inclined fiber-coupled laser beams, which create a stable trapping position below the intersections [143].…”

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confidence: 99%

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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Classification of fetal and adult red blood cells based on hydrodynamic deformation and deep video recognition

Kampen,

Støttrup-Als,

Bruun-Andersen

et al. 2023

Biomed Microdevices

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Flow based deformation cytometry has shown potential for cell classification. We demonstrate the principle with an injection moulded microfluidic chip from which we capture videos of adult and fetal red blood cells, as they are being deformed in a microfluidic chip. Using a deep neural network - SlowFast - that takes the temporal behavior into account, we are able to discriminate between the cells with high accuracy. The accuracy was larger for adult blood cells than for fetal blood cells. However, no significant difference was observed between donors of the two types.

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Deformation of single cells - optical two-beam traps and more

Cited by 2 publications

References 36 publications

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Classification of fetal and adult red blood cells based on hydrodynamic deformation and deep video recognition

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