Out-plane weak ferromagnetic (OWFM) spin arrangements with topological properties can realize a series of interesting physical properties. However, this spin structure tends to exist at low temperatures. The OWFM structure can also be induced at room temperature by hydrostatic pressure, but this isotropic approach tends to form helical AFM structures. We report the OWFM spin arrangement in single crystal Mn3Sn by an anisotropic strategy of high-stress-constrained compression deformation at room temperature. Both experiments and theoretical simulations show that the alignment of OWFM spin structure is due to the distortion of the atomic scale caused by the strain energy during deformation. The OWFM spin arrangement can significantly change the magnetic property of Mn3Sn. As result, the value of remanent magnetization (M
r) for the deformed sample (0.056 mB/f.u.) is about eleven times that for the pre-deformed sample (0.005 mB/f.u.), and the value of coercivity (H
c) increases from 0 kOe (pre-deformed sample) to 6.02 kOe (deformed sample). Our findings provide a way to generate OWFM spin structure at room temperature and may give fresh ideas for creating antiferromagnetic materials with excellent physical properties.