Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world.

Dube, Syamalima; Bachman, Suzanne; Spicer, Tim; Love, Jayne L.; Choi, David; Esteban, Eduardo; Ferrer, Jorge F.; Poiesz, Bernard J.

doi:10.1099/0022-1317-78-6-1389

Cited by 34 publications

(25 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A consensus sequence was obtained with the remaining sequences to recalculate the substitution numbers, and 31 nucleotide substitutions were observed, with an average of 7.7 per sequence (1.4 %), most of them silent (74.2 %) and transitions (83.8 %). These results are similar to these obtained in previous reports (Coulston et al 1990, Dube et al 1997, Dube et al 2000, Camargos et al 2002. The sequences D00647 and K02120 were excluded from aminoacid alignment (Fig.…”

Section: Resultssupporting

confidence: 90%

See 1 more Smart Citation

Molecular Characterization of Partial Sequences of Brazilian BLV Gag Sequences

Camargos¹,

Reis²,

Leite³

2007

VR&R

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 90%

“…In all sequences deletions and insertions were observed. Insertions and deletions were not observed in BLV pol and env sequences (Coulston et al 1990, Dube et al 1997, Dube et al 2000, Camargos et al 2002, Monti et al 2005. The sequences D00647 and K02120 probably had sequencing errors, as mentioned by others (Dube et al 2000).…”

Section: Resultsmentioning

confidence: 70%

Molecular Characterization of Partial Sequences of Brazilian BLV Gag Sequences

Camargos¹,

Reis²,

Leite³

2007

VR&R

View full text Add to dashboard Cite

show abstract

“…This may be due to the small number of available sequences and/or limited genetic variation. Some authors performed phylogenetic analyses with sequences of the BLV pol gene and HTLV-1 p21e gene, which proved inadequate to distinguish between viral strains [48,49]. The genetic variation of Primate T Leukemia Virus 1 (PTLV-1) in the env gene was greater than that of HTLV-II and BLV [50].…”

Section: Molecular Characterizationmentioning

confidence: 98%

Molecular characterization of the env gene from Brazilian field isolates of Bovine Leukemia Virus

Camargos¹,

Pereda

Stancek

et al. 2007

Virus Genes

View full text Add to dashboard Cite

Molecular characterization of Bovine leukemia virus (BLV) isolates from Brazil using the env gene sequences revealed a high conservation of this gene. In most cases the substitutions corresponded to silent transitions. In addition, cystein residues, potential glycosylation sites, neutralization domains and other critical residues involved with the envelope structural domains and viral infectivity were conserved. Most of the substitutions found in the aminoacid sequences of the gp51 protein were localized in the G and H epitopes. Using the SIFT software, it was predicted that they should not alter the protein functions. Phylogenetic analyses showed that partial or complete env gene sequences grouped in three or four phylogenetic clusters, respectively. The sequences from the Brazilian isolates had similar mutation rates as compared to samples from other countries, and belonged to at least two phylogenetic clusters.

show abstract

“…In the present study, although we do not have sequence data, we can predict that the Argentine-type isolate also seems to belong to the Japanese-American subgroup. Dube et al (1997), have recently reported the phylogeny of BLV using a BLV specific PCR assay, and provided very useful information about the highly conserved BLV sequences in the env gene.…”

Section: Discussionmentioning

confidence: 99%

A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction

González

Norimine

Valera³

et al. 1999

Pesq. Vet. Bras.

View full text Add to dashboard Cite

Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). In Argentina, where a program to eradicate EBL has been introduced, sensitive and reliable diagnosis has attained high priority. Although the importance of the agar gel immunodiffusion test remains unchanged for routine work, an additional diagnostic technique is necessary to confirm cases of sera with equivocal results or of calves carrying maternal antibodies.Utilizing a nested shuttle polymerase chain reaction, the proviral DNA was detected from cows experimentally infected with as little as 5 ml of whole blood from BLV seropositive cows that were nonetheless normal in haematological terms. It proved to be a very sensitive technique, since it rapidly revealed the presence of the provirus, frequently at 2 weeks postinoculation and using a two-round procedure of nested PCR taking only 3 hours. Additionally, the primers used flanked a portion of the viral genome often employed to differentiate BLV type applying BamHI digestion. It is concluded that this method might offer a highly promising diagnostic tool for BLV infection.

show abstract

Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world.

Cited by 34 publications

References 33 publications

Molecular Characterization of Partial Sequences of Brazilian BLV Gag Sequences

Molecular Characterization of Partial Sequences of Brazilian BLV Gag Sequences

Molecular characterization of the env gene from Brazilian field isolates of Bovine Leukemia Virus

A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction

Contact Info

Product

Resources

About