Degenerative disc disease in young adults: cytokine profile and angiogenic factors

Novikova, A. V.; Pravdyuk, N. G.; Saklakova, VS; Лоломадзе, Е. А.; Feniksov, VM; Nikolaev, DA; Davygora, KS; Timofeev, V. T.; Na, Shostak

doi:10.24075/brsmu.2021.061

Cited by 6 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A cascade of immune inflammation penetrates disc from the bone marrow of the vertebral bodies through damaged EP. The probability of an inflammatory reaction in the bone in situ is low due to the fact that Modic-changes are detected in patients exclusively in the presence of hernia and/or erosion of the EP, which has been demonstrated in similar studies [18,19]. In turn, the feedback of the EP bone part with the state of the NP cells, their activity and influence on the hydrophilic properties of the extracellular matrix has been demonstrated in studies on the nutritional function of the EP [20].…”

Section: Discussionmentioning

confidence: 81%

Immunomorphogenesis in degenerative disc disease: the role of proinflammatory cytokines and angiogenesis factors

Pravdyuk

Novikova

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Back pain (BP) due to degenerative spinal injury is a severe, often disabling disease and it tends to decrease age of onset. The cellular and molecular basis of the disc and adjacent structures degradation remains in the focus of pathophysiologists and pathomorphologists close attention. The aim of the study was to determine the expression level of proinflammatory cytokines (IL-1β, IL-6, IL-17) and angiogenesis markers (VEGF-A and CD31) in intervertebral disc (IVD) tissue, their association between each other and between disc degeneration in young people with discogenic BP. In patients who underwent discectomy for a disc herniation, a clinical examination, magnetic resonance imaging (MRI) of the lumbar spine, histological and immunohistochemical analyses of these factors in the disc tissue were performed in comparison with the parameters of healthy group samples (controls). Histology image analysis of IVD fragments of the degenerative disc disease (DDD) group detected zones of inflammatory infiltration, combined with vascularization, the presence of granulation tissue and clusters of chondrocytes in the tissue of nucleus pulposus (NP). Statistically significant expression of IL-1β, IL-6, IL-17 and VEGF-A (especially on the chondrocytes surface) and CD31 (in the disc matrix) was evident in the samples of the DDD group compared with the controls (p < 0.0001), that showed a strong correlation with the histological disc degeneration stage (r > 0.5; p < 0.0001). This denotes a high immunoinflammatory potential of chondrocytes and demonstrates their altered morphogenetic properties. The coincidence of the spatial expression of IL-1β and IL-17 in the perivascular zone endothelium and in the vascular lumen (p < 0.01) was found, which point at the inflammatory synergy of these cytokines. High expression of VEGF-A prevailed on the surface of chondrocytes in cell clusters compared to the matrix (p < 0.0001), indicating that the NP cells trigger the angiogenesis. The absence of CD31 in the cracks of NP with high expression in the disc matrix states the secondary nature of IVD defects due to degeneration, and not due to vascular ingrowth. A high number of patients with Modic changes according to MRI data shown the contribution of cytokines to the formation of reactive spondylitis and the clinical course of BP. These results obtained will help to development molecular/cellular targets and basic strategies for therapy of BP with DDD in the early stages in young people.

show abstract

Section: Discussionmentioning

confidence: 81%

Immunomorphogenesis in degenerative disc disease: the role of proinflammatory cytokines and angiogenesis factors

Pravdyuk

Novikova

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…A cascade of immune inflammation penetrates disc from the bone marrow of the vertebral bodies through damaged EP. The probability of an inflammatory reaction in the bone in situ is low due to the fact that Modic changes are detected in patients exclusively in the presence of hernia and/or erosion of the EP, which has been demonstrated in similar studies [ 30 , 31 ]. In turn, the feedback of the EP bone part with the state of the NP cells, their activity and influence on the hydrophilic properties of the extracellular matrix has been demonstrated in studies on the nutritional function of the EP [ 32 ].…”

Section: Discussionmentioning

confidence: 86%

Immunomorphogenesis in Degenerative Disc Disease: The Role of Proinflammatory Cytokines and Angiogenesis Factors

et al. 2023

View full text Add to dashboard Cite

Back pain (BP) due to degenerative disc disease (DDD) is a severe, often disabling condition. The aim of this study was to determine the association between the expression level of proinflammatory cytokines (IL-1β, IL-6, and IL-17), angiogenesis markers (VEGF-A and CD31) in intervertebral disc (IVD) tissue and IVD degeneration in young people with discogenic BP. In patients who underwent discectomy for a disc herniation, a clinical examination, magnetic resonance imaging of the lumbar spine, histological and immunohistochemical analyses of these factors in IVD were performed in comparison with the parameters of healthy group samples (controls). Histology image analysis of IVD fragments of the DDD group detected zones of inflammatory infiltration, combined with vascularization, the presence of granulation tissue and clusters of chondrocytes in the tissue of nucleus pulposus (NP). Statistically significant increased expression of IL-1β, IL-6, IL-17, VEGF-A and CD31 was evident in the samples of the DDD group compared with the controls, that showed a strong correlation with the histological disc degeneration stage. Our results denote an immunoinflammatory potential of chondrocytes and demonstrates their altered morphogenetic properties, also NP cells may trigger the angiogenesis.

show abstract

The problem of choice of therapy for a patient with dorsalgia

et al. 2023

View full text Add to dashboard Cite

Musculoskeletal pain syndromes are one of the most common causes of temporary disability, they are often associated with a significant decrease in the quality of life of patients. Due to the peculiarities of biomechanics (significant physical exertion experienced throughout life, a large volume of movements in various directions), the lumbar spine is especially vulnerable, the lesion of which is often associated with the development of lumbar pain (PB). The mechanisms of formation of PB are diverse, however, as a rule, inflammation is the basis of the pain syndrome. The results of modern studies have convincingly demonstrated the presence of imaging and biochemical markers of the inflammatory process in the area of altered spinal structures, in particular, in intervertebral discs and arch-process joints. There are reasons to believe that it is the focus of inflammation that can be the source of pain, although in the future the role of the active inflammatory process may become less important, and other mechanisms are involved in maintaining pain and other clinical manifestations. In this regard, drugs should be chosen for the treatment of patients with PB, depending on the predominant action - analgesic or anti-inflammatory. In the article, along with the main mechanisms of the occurrence and persistence of PB, modern approaches to the treatment of such patients are considered. The undoubted validity of the use of nonsteroidal anti-inflammatory drugs (NSAIDs) for the treatment of patients with PB is noted. Their most significant clinical effects are noted, which may be useful in the treatment of patients with PB. The features of side effects associated with the use of NSAIDs, including those from the gastrointestinal tract and the cardiovascular system, are considered. Information is provided on the results of studies devoted to the study of the efficacy and safety of dexketoprofen (Dexonal®, Binnopharm Group) in the treatment of patients with PB. The undoubted positive properties of the drug are noted (rapid development of action with a powerful analgesic effect and a favorable safety profile) Dexonal®.

show abstract

Degenerative disc disease in young adults: cytokine profile and angiogenic factors

Cited by 6 publications

References 28 publications

Immunomorphogenesis in degenerative disc disease: the role of proinflammatory cytokines and angiogenesis factors

Immunomorphogenesis in degenerative disc disease: the role of proinflammatory cytokines and angiogenesis factors

Immunomorphogenesis in Degenerative Disc Disease: The Role of Proinflammatory Cytokines and Angiogenesis Factors

The problem of choice of therapy for a patient with dorsalgia

Contact Info

Product

Resources

About