Degradation and release of tannic acid from an injectable tissue regeneration bead matrix in vivo

Abstract

The development of multifunctional biomaterials as both tissue regeneration and drug delivery devices is currently a major focus in biomedical research. Tannic Acid (TA), a naturally occurring plant polyphenol, displays unique medicinal abilities as an antioxidant, an antibiotic, and as an anticancer agent. TA has applications in biomaterials acting as a crosslinker in polymer hydrogels improving thermal stability and mechanical properties. We have developed injectable cell seeded collagen beads crosslinked with TA for breast reconstruction and anticancer activity following lumpectomy. This study determined the longevity of the bead implants by establishing a degradation time line and TA release profile in vivo. Beads crosslinked with 0.1% TA and 1% TA were compared to observe the differences in TA concentration on degradation and release. We found collagen/TA beads degrade at similar rates in vivo, yet are resistant to complete degradation after 16 weeks. TA is released over time in vivo through diffusion and cellular activity. Changes in mechanical properties in collagen/TA beads before implantation to after 8 weeks in vivo also indicate loss of TA over a longer period of time. Elastic moduli decreased uniformly in both 0.1% and 1% TA beads. This study establishes that collagen/TA materials can act as a drug delivery system, rapidly releasing TA within the first week following implantation. However, the beads retain TA long term allowing them to resist degradation and remain in situ acting as a cell scaffold and tissue filler. This confirms its potential use as an anticancer and minimally invasive breast reconstructive device following lumpectomy.

Wound Healing Properties of Natural Products: Mechanisms of Action

“…Polyphenols have also been used in biomaterials to evaluate their wound healing properties. For example, Baldwin et al [ 75 ] applied tannic acid incorporated from 0.1% to 1% in collagen beads. The tannic acid-collagen beads have a wound healing effect, due to the antibacterial properties of tannic acid and might be of potential implementation in filling surgical voids at 16 weeks.…”

Emerging Bioactive Agent Delivery-Based Regenerative Therapies for Lower Genitourinary Tissues

“…Adding small molecules with expected features, combining two or more biomaterials, crosslinking, and chemically integrating functional groups, segments, or molecules are practical methods employed to achieve additional characteristics for biomaterials used as delivery systems (Figure 3) [15,[64][65][66][67]. For example, a sonosensitive emulsion was doped in fibrin matrix for the development of an acoustically-responsive delivery system (ARDS) to control angiogenic processes [68].…”

Designing of Polymeric Nanoparticles for Enhanced Breast Cancer Therapy: Combining Paclitaxel, Boric Acid and Tannic Acid for Controlled Drug Delivery

“…TA has recently stood out as a contributing molecule that enhances the solubility of various hydrophobic drugs (such as amphotericin B, curcumin, rapamycin, PTX or paclitaxel, and docetaxel). [35][36][37][38] This study focuses on the preparation of nanoparticulate systems containing PTX, BA, and polyphenolic TA using the emulsion technique, a simple, cost-effective, and straightforward method with Gel and NaAlg polymers. The anticancer and antioxidant activities of the prepared polymer agents were determined using the MTT and DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging capacity methods, respectively.…”