Degradation and Transformability of DNA from Transgenic Leaves

Ceccherini, Maria Teresa; Poté, John; Kay, Elisabeth; Van, Van Trân; Maréchal, J; Pietramellara, Giacomo; Nannipieri, Paolo; Vogel, Timothy M.; Simonet, Pascal

doi:10.1128/aem.69.1.673-678.2003

Cited by 88 publications

(59 citation statements)

References 34 publications

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“…A few published studies describe processes that limit or induce DNA release from plastids or mitochondria (Nielsen et al, 2001;Thorsness et al, 1993;Vincent et al, 1988). Ceccherini et al (2003) reported over 98% degradation of a chloroplast-harbored gene, as measured by PCR amplification, over a 72 h period in ground tobacco leaf material. Another study by the same group provided evidence that Acinetobacter sp.…”

Section: Release Of Organelle Dna From Plantsmentioning

confidence: 99%

Release and persistence of extracellular DNA in the environment

Nielsen

Johnsen

Bensasson

et al. 2007

Environ. Biosafety Res.

484

382

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The introduction of genetically modified organisms (GMOs) has called for an improved understanding of the fate of DNA in various environments, because extracellular DNA may also be important for transferring genetic information between individuals and species. Accumulating nucleotide sequence data suggest that acquisition of foreign DNA by horizontal gene transfer (HGT) is of considerable importance in bacterial evolution. The uptake of extracellular DNA by natural transformation is one of several ways bacteria can acquire new genetic information given sufficient size, concentration and integrity of the DNA. We review studies on the release, breakdown and persistence of bacterial and plant DNA in soil, sediment and water, with a focus on the accessibility of the extracellular nucleic acids as substrate for competent bacteria. DNA fragments often persist over time in many environments, thereby facilitating their detection and characterization. Nevertheless, the long-term physical persistence of DNA fragments of limited size observed by PCR and Southern hybridization often contrasts with the short-term availability of extracellular DNA to competent bacteria studied in microcosms. The main factors leading to breakdown of extracellular DNA are presented. There is a need for improved methods for accurately determining the degradation routes and the persistence, integrity and potential for horizontal transfer of DNA released from various organisms throughout their lifecycles.

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Section: Release Of Organelle Dna From Plantsmentioning

confidence: 99%

Release and persistence of extracellular DNA in the environment

Nielsen

Johnsen

Bensasson

et al. 2007

Environ. Biosafety Res.

484

382

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“…The state of competence leading to natural transforma-bility is commonly related to the presence of competence genes, like the com genes present in Thermus thermophilus and Acinetobacter baylyi (Friedrich et al, 2001 and. The naturally transformable strain A. baylyi ADP1 (ADP1; also known as A. baylyi BD413, see Vaneechoutte et al, 2006) has been demonstrated to develop genetic competence and to be transformable in soil (Ceccherini et al, 2003;Kay et al, 2003;Nielsen et al, 2000b). Transformants successfully occurred after treatment with DNA homologous to recipient genomes (Nielsen et al, 2000a).…”

Section: Introductionmentioning

confidence: 99%

Assessment of transformability of bacteria associated with tomato and potato plants

Overbeek

Ray

Elsas

2007

Environ. Biosafety Res.

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Transformation of plant-associated bacteria by plant DNA has never been demonstrated in agricultural fields. In total 552 bacterial isolates from stems of Ralstonia solanacearum-infected and healthy tomato plants and from stems and leaves of healthy potato plants were tested for natural genetic competence using plasmid pSKTG DNA and homologous DNA extracts. Control strain Acinetobacter baylyi ADP1 was transformable with both DNA extracts. No transformable isolates were observed after treatment with plasmid pSKTG DNA. Two isolates, P34, identified as Pseudomonas trivialis and A19, identified as Pseudomonas fragi, were selected on the basis of the consistently higher Rp-resistant CFU numbers after treatment with DNA from Rp-resistant cells than with that from wild-type cells. P34 showed 2.1-fold and A19 1.5-fold higher Rp-resistant CFU numbers after treatment with DNA from homologous Rp-resistant cells versus that from wild-type cells. It is concluded that bacteria capable of in vitro capture and integration of exogenous DNA into their genomes are relatively rare in culturable bacterial communities associated with tomato and potato plants, or that conditions conducive to transformation were not met in transformation assays.

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“…The data demonstrated that under specific conditions, horizontal gene transfer can take place in planta. In a second step, the in situ transformation capacity of transplastomic plant DNA was studied (Ceccherini et al 2003). Here, the DNA purified from decaying or enzymatically treated leaf tissue was found to degrade within 72 h but was able to transform in vitro the above Acinetobacter strain at a low frequency (between 10…”

Section: Biosafety Of Transplastomic Plantsmentioning

confidence: 99%

Transplastomic plants for innovations in agriculture. A review

Wani

Sah

Sági

et al. 2015

Agron. Sustain. Dev.

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Food production has to be significantly increased in order to feed the fast growing global population estimated to be 9.1 billion by 2050. The Green Revolution and the development of advanced plant breeding tools have led to a significant increase in agricultural production since the 1960s. However, hundreds of millions of humans are still undernourished, while the area of total arable land is close to its maximum utilization and may even decrease due to climate change, urbanization, and pollution. All these issues necessitate a second Green Revolution, in which biotechnological engineering of economically and nutritionally important traits should be critically and carefully considered. Since the early 1990s, possible applications of plastid transformation in higher plants have been constantly developed. These represent viable alternatives to existing nuclear transgenic technologies, especially due to the better transgene containment of transplastomic plants. Here, we present an overview of plastid engineering techniques and their applications to improve crop quality and productivity under adverse growth conditions. These applications include (1) transplastomic plants producing insecticidal, antibacterial, and antifungal compounds. These plants are therefore resistant to pests and require less pesticides. (2) Transplastomic plants resistant to cold, drought, salt, chemical, and oxidative stress. Some pollution tolerant plants could even be used for phytoremediation. (3) Transplastomic plants having higher productivity as a result of improved photosynthesis. (4) Transplastomic plants with enhanced mineral, micronutrient, and macronutrient contents. We also evaluate field trials, biosafety issues, and public concerns on transplastomic plants. Nevertheless, the transplastomic technology is still unavailable for most staple crops, including cereals. Transplastomic plants have not been commercialized so far, but if this crop limitation were overcome, they could contribute to sustainable development in agriculture.

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Degradation and Transformability of DNA from Transgenic Leaves

Cited by 88 publications

References 34 publications

Release and persistence of extracellular DNA in the environment

Release and persistence of extracellular DNA in the environment

Assessment of transformability of bacteria associated with tomato and potato plants

Transplastomic plants for innovations in agriculture. A review

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