Degradation behaviour of block copolymers containing poly(lactic-glycolic acid) and poly(ethylene glycol) segments

Penco, Maurizio; Marcioni, Silvia; Ferruti, Paolo; D’Antone, Salvatore; Deghenghi, Romano

doi:10.1016/0142-9612(95)00323-1

Cited by 68 publications

(56 citation statements)

References 15 publications

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“…[29][30][31][32][33] However, they have not been entirely successful because of their limited range of physical properties and their tendency either to degrade into toxic products or to cause adverse tissue reactions. [9][10][11][12][13] Our results demonstrate that the LDIglucose polymer possesses many attributes that are potentially useful in tissue-engineering and other biomedical applications. This is evident by the fact that LDI synthesized from lysine ethyl ester dihydrochloride is of high purity and can be obtained in high yields to minimize the presence of polymer contaminants.…”

Section: Discussionmentioning

confidence: 87%

“…[9][10][11][12][13] To examine whether the breakdown products of the LDI-glucose polymer decrease the pH of the surrounding solution, we measured over a period of 60 days the pH of the PBS containing polymer at 10 mg/mL. We selected PBS as the medium because its buffering capacity resembles that of biological fluids.…”

Section: Biodegradability Of Ldi-glucose Matrixmentioning

confidence: 99%

“…A total of 0.5 μg of RNA was mixed with 1 μg of oligo(dT) (12)(13)(14)(15)(16)(17)(18) oligomer; PerkinElmer) in reverse transcription buffer and incubated for 10 min at room temperature. Thereafter, the reaction mixture was cooled on ice and incubated with 200 U of Moloney murine leukemia virus (Mo-MuLV) reverse transcriptase for 60 min at 37°C.…”

Section: Comparison Of Mrna Expression For Collagen Type I and Transfmentioning

confidence: 99%

“…This was due either to the contaminants present in the hard segment preparations or to the inherent problems associated with the soft segments. [9][10][11][12][13] In this study, we redesigned the urethane polymer to degrade to nontoxic products. Our goal is a polyurethane for biomedical applications that is biodegradable and biocompatible.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis, Biodegradability, and Biocompatibility of Lysine Diisocyanate–Glucose Polymers

et al. 2002

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The success of a tissue-engineering application depends on the use of suitable biomaterials that degrade in a timely manner and induce the least immunogenicity in the host. With this purpose in mind, we have attempted to synthesize a novel nontoxic biodegradable lysine diisocyanate (LDI)-and glucose-based polymer via polymerization of highly purified LDI with glucose and its subsequent hydration to form a spongy matrix. The LDI-glucose polymer was degradable in aqueous solutions at 37, 22, and 4°C, and yielded lysine and glucose as breakdown products. The degradation products of the LDI-glucose polymer did not significantly affect the pH of the solution. The physical properties of the polymer were found to be adequate for supporting cell growth in vitro, as evidenced by the fact that rabbit bone marrow stromal cells (BMSCs) attached to the polymer matrix, remained viable on its surface, and formed multilayered confluent cultures with retention of their phenotype over a period of 2 to 4 weeks. These observations suggest that the LDI-glucose polymer and its degradation products were nontoxic in vitro. Further examination in vivo over 8 weeks revealed that subcutaneous implantation of hydrated matrix degraded in vivo three times faster than in vitro. The implanted polymer was not immunogenic and did not induce antibody responses in the host. Histological analysis of the implanted polymer showed that LDIglucose polymer induced a minimal foreign body reaction, with formation of a capsule around the degrading polymer. The results suggest that biodegradable peptide-based polymers can be synthesized, and may potentially find their way into biomedical applications because of their biodegradability and biocompatibility.

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Section: Discussionmentioning

confidence: 87%

Section: Biodegradability Of Ldi-glucose Matrixmentioning

confidence: 99%

Section: Comparison Of Mrna Expression For Collagen Type I and Transfmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis, Biodegradability, and Biocompatibility of Lysine Diisocyanate–Glucose Polymers

et al. 2002

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“…The degradation products of polymers made of poly-lactide and poly-glycolide create an acidic environment in vivo [20][21][22]. To examine whether the breakdown products of the LDI-glycerol polymer also change the pH of the surrounding solution, we measured the pH of the PBS containing 1 mg/ml polymer over a period of 60 days.…”

Section: Resultsmentioning

confidence: 99%

A new peptide-based urethane polymer: synthesis, biodegradation, and potential to support cell growth in vitro

et al. 2000

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A novel non-toxic biodegradable lysine-di-isocyanate (LDI)-based urethane polymer was developed for use in tissue engineering applications. This matrix was synthesized with highly purified LDI made from the lysine diethylester. The ethyl ester of LDI was polymerized with glycerol to form a prepolymer. LDI-glycerol prepolymer when reacted with water foamed with the liberation of CO 2 to provide a pliable spongy urethane polymer. The LDI-glycerol matrix degraded in aqueous solutions at 100, 37, 22, and 4°C at a rate of 27.7, 1.8, 0.8, and 0.1 mM per 10 days, respectively. Its thermal stability in water allowed its sterilization by autoclaving. The degradation of the LDI-glycerol polymer yielded lysine, ethanol, and glycerol as breakdown products. The degradation products of LDI-glycerol polymer did not significantly affect the pH of the solution. The glass transition temperature (T g ) of this polymer was found to be 103.4°C. The physical properties of the polymer network were found to be adequate to support the cell growth in vitro, as evidenced by the fact that rabbit bone marrow stromal cells (BMSC) attached to the polymer matrix and remained viable on its surface. Culture of BMSC on LDI-glycerol matrix for long durations resulted in the formation of multilayered confluent cultures, a characteristic typical of bone cells. Furthermore, cells grown on LDI-glycerol matrix did not differ phenotypically from the cells grown on the tissue culture polystyrene plates as assessed by the cell growth, and expression of mRNA for collagen type I, and transforming growth factor-β1 (TGF-β1). The observations suggest that biodegradable peptide-based urethane polymers can be synthesized which may pave their way for possible use in tissue engineering applications.

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A review of biodegradable polymers: uses, current developments in the synthesis and characterization of biodegradable polyesters, blends of biodegradable polymers and recent advances in biodegradation studies

Amass¹,

Amass²,

Tighe³

1998

Polym. Int.

921

488

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Degradation behaviour of block copolymers containing poly(lactic-glycolic acid) and poly(ethylene glycol) segments

Cited by 68 publications

References 15 publications

Synthesis, Biodegradability, and Biocompatibility of Lysine Diisocyanate–Glucose Polymers

Synthesis, Biodegradability, and Biocompatibility of Lysine Diisocyanate–Glucose Polymers

A new peptide-based urethane polymer: synthesis, biodegradation, and potential to support cell growth in vitro

A review of biodegradable polymers: uses, current developments in the synthesis and characterization of biodegradable polyesters, blends of biodegradable polymers and recent advances in biodegradation studies

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