Degradation Characteristics of Pinoxaden by Acinetobacter and Prediction of Related Genes

Abstract

In order to improve crop yield per unit area, pinoxaden was widely used in weed control in the field to better degrade the residual pinoxaden in the soil. In this experiment, 14 strains were domesticated and isolated from soil samples using pinoxaden as the only carbon and nitrogen source, and the highly degraded strain was T4P20-1. The effects of different pH values, inoculation amount and culture time on the growth and degradation rate of strain T4P20-1 were studied. The optimal growth conditions of strain T4P20-1 were determined, and the degradation rate reached 92.23%. The morphological characteristics of strain T4P20-1 were determined by optical microscope. The strain T4P20-1 was identified as Acinetobacter Pittii by physiological and biochemical characteristics and 16S rDNA homology analysis, and the nucleic acid sequence was submitted to Gentbank to obtain the accession number MW350066. Through gene sequencing, the whole genome framework of strain T4P20-1 and database annotations of GO, KEGG, COG, CAZymes and PHI were obtained. Meanwhile, genes related to herbicide degradation were predicted by gene functional annotations. There was a negative correlation between herbicide and seed germination rate, bud length and root length of maize susceptible to subsequent crop. In conclusion, the strains screened in this study have certain remediation effects on soil contaminated by pinoxaden. It provides a new choice for the degradation of pinoxaden in the future. It can increase crop yields and reduce food security risks.

Oil-Destructor Bacteria With Resistance to the Presence of Additional Pollutants

“…Особый интерес в этом отношении представляют бактерии рода Acinetobacter. Они распространены повсеместно и в качестве источника углерода могут использовать очень широкий круг веществ, что позволяет им участвовать в биодеградации значительного числа токсикантов, в том числе различных углеводородов [6,7] и пестицидов [8][9][10]. Поэтому представляется интересным и актуальным выяснить, можно ли применять представителей этого рода для биоремедиации пахотных почв, загрязненных нефтью и гербицидами.…”