Degradation, cytotoxicity, and biocompatibility of NIPAAm‐based thermosensitive, injectable, and bioresorbable polymer hydrogels

Cui, Zhanwu; Lee, Bae Hoon; Pauken, Christine; Vernon, Brent L.

doi:10.1002/jbm.a.33093

Cited by 88 publications

(61 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additionally the attached SILY, combined with the electrostatic attraction between the KAFAK and the particle, could hinder the release of any KAFAK that was successfully loaded into the core, resulting in the incomplete peptide release that was observed with these particles. Work has been done, in our lab and others, on the synthesis of degradable poly(NIPAM) nanoparticles (55)(56)(57)(58)(59)(60), and it is likely that the use of a similar system in these particles would result in a more complete peptide release profile over a longer time period.…”

Section: Discussionmentioning

confidence: 99%

Prevention of Collagen-Induced Platelet Binding and Activation by Thermosensitive Nanoparticles

McMasters

Panitch

2015

AAPS J

View full text Add to dashboard Cite

ABSTRACT. Peripheral artery disease is an atherosclerotic occlusion in the peripheral vasculature that is typically treated via percutaneous transluminal angioplasty. Unfortunately, deployment of the angioplasty balloon damages the endothelial layer, exposing the underlying collagen and allowing for the binding and activation of circulating platelets, which initiate an inflammatory cascade leading to eventual restenosis. Here, we report on the development of poly(NIPAm-MBA-AMPS-AAc) nanoparticles that have a collagen I-binding peptide crosslinked to their surface allowing them to bind to exposed collagen. Once bound, these particles mask the exposed collagen from circulating platelets, effectively reducing collagen-mediated platelet activation. Using collagen I-coated plates, we demonstrate that these particles are able to bind to collagen at concentrations above 0.5 mg/mL. Once bound, these particles inhibit collagen-mediated platelet activation by over 60%. Using light scattering and zeta potential measurements, we investigated the potential of the nanoparticles as a drug delivery platform. We have verified that the collagen-binding nanoparticles retain the temperature sensitivity common to poly(NIPAm)-based nanoparticles while remaining colloidally stable in aqueous environments. We also demonstrate that they are able to passively load and release anti-inflammatory cell penetrating peptides. Combined, we have developed a collagen-binding nanoparticle that has dual therapy potential, preventing collagen-mediated platelet activation while delivering water-soluble therapeutics directly to the damaged area.

show abstract

Section: Discussionmentioning

confidence: 99%

Prevention of Collagen-Induced Platelet Binding and Activation by Thermosensitive Nanoparticles

McMasters

Panitch

2015

AAPS J

View full text Add to dashboard Cite

show abstract

“…Experiments have demonstrated that this hydrogel exhibited an LCST of 27°C and a swelling ratio of 150% before degradation. However, mass remaining reduced to 0 in 20 days both in vitro 27 and in vivo, 28 due to the hydrolysis of DBA leading to an increase of LCST. The rapid degradation ratio is detrimental to the adhesion and proliferation of the cells.…”

Section: Introductionmentioning

confidence: 99%

Injectable hydrogel as stem cell scaffolds from the thermosensitive terpolymer of NIPAAm/AAc/HEMAPCL

Lian

Xiao²,

Bian³

et al. 2012

IJN

View full text Add to dashboard Cite

A series of biodegradable thermosensitive copolymers was synthesized by free radical polymerization with N-isopropylacrylamide (NIPAAm), acrylic acid (AAc) and macromer 2-hydroxylethyl methacrylate-poly(ε-caprolactone) (HEMAPCL). The structure and composition of the obtained terpolymers were confirmed by proton nuclear magnetic resonance spectroscopy, while their molecular weight was measured using gel permeation chromatography. The copolymers were dissolved in phosphate-buffered saline (PBS) solution (pH = 7.4) with different concentrations to prepare hydrogels. The lower critical solution temperature (LCST), cloud point, and rheological property of the hydrogels were determined by differential scanning calorimetry, ultraviolet-visible spectrometry, and rotational rheometry, respectively. It was found that LCST of the hydrogel increased significantly with the increasing NIPAAm content, and hydrogel with higher AAc/HEMAPCL ratio exhibited better storage modulus, water content, and injectability. The hydrogels were formed by maintaining the copolymer solution at 37°C. The degradation experiment on the formed hydrogels was conducted in PBS solution for 2 weeks and demonstrated a less than 20% weight loss. Scanning electron microscopy was also used to study the morphology of the hydrogel. The copolymer with NIPAAm/AAc/HEMAPCL ratio of 88:9.6:2.4 was bioconjugated with type I collagen for the purpose of biocompatibility enhancement. In-vitro cytotoxicity of the hydrogels both with and without collagen was also addressed.

show abstract

“…299,[305][306][307][308][309][310] However, PNIPAM notably shows cytotoxity and is non-biodegradable, 311 which limits its applications in vivo, even though PNIPAM with finite molecular weight can be eliminated via glomerular filtration without in vivo long-term accumulation. To overcome these drawbacks, PNIPAM hydrogels have been established as cell sheets to construct organized hepatocyte or endothelial mono-or multi-layers.…”

Section: Peg and Poly(n-isopropylacrylamide)mentioning

confidence: 99%

Stimuli-Responsive InjectableIn situ-Forming Hydrogels for Regenerative Medicines

Kim

Kwon

et al. 2015

Polymer Reviews

View full text Add to dashboard Cite

Research on injectable in situ-forming hydrogels has been conducted in diverse biomedical applications for a long period. These hydrogels exhibit sol-to-gel phase transition in accordance with the external stimuli such as temperature change. Also, unlike the traditional surgical procedures the hydrogels have the distinct properties of easy management and minimal invasiveness via simple aqueous state injections at target sites. Currently, numerous polymer materials have been reported as potential stimulusinduced in situ-forming hydrogels. In this review, a comprehensive overview of the state-of-the-art of these rapidly developing materials has been outlined. In situ-forming hydrogels formed by electrostatic and hydrophobic interactions as well as their mechanistic characteristics and biomedical applications in regenerative medicine have also been discussed. The review concludes with perspectives on the future of stimulus-induced in situ-forming hydrogels.

show abstract

Degradation, cytotoxicity, and biocompatibility of NIPAAm‐based thermosensitive, injectable, and bioresorbable polymer hydrogels

Cited by 88 publications

References 32 publications

Prevention of Collagen-Induced Platelet Binding and Activation by Thermosensitive Nanoparticles

Prevention of Collagen-Induced Platelet Binding and Activation by Thermosensitive Nanoparticles

Injectable hydrogel as stem cell scaffolds from the thermosensitive terpolymer of NIPAAm/AAc/HEMAPCL

Stimuli-Responsive InjectableIn situ-Forming Hydrogels for Regenerative Medicines

Contact Info

Product

Resources

About