Degradation effect of carbendazim in soil by application with the microbial agent, Rhodococcus sp. 3-2

Abstract

BACKGROUND:The fungicide of benomyl, a benzimidazole group, has been commonly used for pesticides against fungal diseases in the world. However, benomyl is rapidly hydrolyzed in the environment after using to control plant diseases and has adverse effects by generating carbendazim, which is toxic to plants, humans, and the environment. METHODS AND RESULTS: In this study, the decomposition effect of carbendazim, a degradation product of benomyl was conducted in pot and field after making a pro-totype of benomyl-degrading microbial agent (BDMA). We found that the carbendazim-degrading microbial agent (CDMA) (10 5 , 10 6 , and 10 7 cfu/g soil) decomposed carbendazim by 50% or more in all the treatments, compared to the untreated control in the pot tests after four weeks. The effect of 100% decomposition of carbendazim was observed at 7 days after treatment, when the prototype of BDMA was apllied at 10-folds dilution in the field. The decomposition effect at more than 60% and plant growth promoting effect were observed after 7 days of the treatment, compared with the untreated group in the second field experiment,treated with commercially available concentrations of 500-folds and 1,000-folds. CONCLUSION(S): These results might represent that the BDMA would decompose carbendazim effectively, a de-

Mass Cultivation of Rhodococcus sp. 3-2, a Carbendazim-Degrading Microorganism, and Development of Microbial Agents

“…베노 밀은 자연상태에는 빠르게 카벤다짐으로 분해되며 대부분의 살균성은 카벤다짐의 작용으로 알려져 있다 [9]. 카벤다짐은 화 학적으로 안정하고 비교적 환경에 오래 지속되며 간과 내분비 계에 해를 끼칠 수 있고 동물에게 낮은 농도에서도 돌연변이 유발 및 기형 유발 효과가 있다 [10,11]. 또한 카벤다짐은 베노 밀 살포 후 4년이 지나도 토양 내 잔류가 감지되는 등 긴 환경 지속성을 가지고 있어 토양 내 잔류문제가 커지고 있다.…”

“…최근 연구에서 Rhodococcus sp. 3-2 균주는 액상배지에서 60시간 내에 96% 이상의 베노밀을 분해하였으 며, 토양 처리 시 베노밀과 카벤다짐을 10일 이내에 78~82% 분해하는 것이 밝혀졌다 [11]. 카보퓨란(carbofuran), 카두사포 스(cadusafos) 등의 농약과 동일하게, 카벤다짐 분해에 대한 Rhodococcus sp.…”