Degradation Mechanism of AAA+ Proteases and Regulation of Streptomyces Metabolism

Abstract

Hundreds of proteins work together in microorganisms to coordinate and control normal activity in cells. Their degradation is not only the last step in the cell’s lifespan but also the starting point for its recycling. In recent years, protein degradation has been extensively studied in both eukaryotic and prokaryotic organisms. Understanding the degradation process is essential for revealing the complex regulatory network in microorganisms, as well as further artificial reconstructions and applications. This review will discuss several studies on protein quality-control family members Lon, FtsH, ClpP, the proteasome in Streptomyces, and a few classical model organisms, mainly focusing on their structure, recognition mechanisms, and metabolic influences.

LexA, an SOS response repressor, activates TGase synthesis in Streptomyces mobaraensis

“…FtsH is the membrane-anchored metalloprotease among the AAA+ proteases. FtsH is relatively less studied in Streptomyces, although FtsH is not a key developmental gene of Streptomyces, it has a certain degree of effect on cell differentiation (Xu et al, 2022). DivIVA is located at the tip of the hyphae and is a key structure protein for the polar growth of Streptomyces that enhance polar cell wall synthesis by assembling larger multiprotein complexes (Hempel et al, 2008).…”

“…Plate phenotyping and scanning electron microscopy experiments were conducted to investigate the role of LexA in the maturation of S. mobaraensis spores. From our analysis of predicted target genes, we identified four key genes that are crucial for morphological differentiation: whiB (Davis and Chater, 1992), ssgA (Traag and van Wezel, 2008), divIVA (Passot et al, 2022) and ftsH (Xu et al, 2022) (Supplementary Table S5). To confirm the regulatory impact of LexA on these genes, probes were generated through PCR amplification of their promoter regions (Probe whiBp, ssgAp, divIVAp, and ftsHp).…”

Separation of lysozyme-ovotransferrin complexes and the cooperative role of their components in egg white

“…Proteins have functions such as enzyme activities, and protein complexes made up of these proteins work together to control these functions [1,2]. To investigate the functions of these protein complexes, techniques to separate and/or form these complexes are considered necessary.…”