Degradation of 2-mercaptobenzothizaole in microbial electrolysis cells: Intermediates, toxicity, and microbial communities

San-Martín, María Isabel; Escapa, Adrián; Alonso, Raúl M.; Canle, Moisés; Morán, Antonio

doi:10.1016/j.scitotenv.2020.139155

Cited by 19 publications

(7 citation statements)

References 39 publications

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“…In the present study, we compared the degradation intermediates formed when MBT was degraded by Svi DyP (six different species, Table 4 ) with some of the products formed when MBT is degraded by either treatment method (including chemical methods and peroxidases with and without redox mediators). As summarized in Table 5 , all of the MBT degradation products we found have been previously reported in literature [ 24 , 44 , 45 , 46 , 47 ]. A summary of these intermediates is presented in Figure 7 , illustrating their potential structures as well as possible chemical reactions that could lead to their formation based on literature findings.…”

Section: Resultsmentioning

confidence: 56%

Efficient Degradation of 2-Mercaptobenzothiazole and Other Emerging Pollutants by Recombinant Bacterial Dye-Decolorizing Peroxidases

et al. 2021

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In recent years, concerns are being raised about the potential harmful effects of emerging pollutants (EPs) on human and aquatic lives. Extensive research is being conducted on developing efficient remediation strategies to target this new class of toxic pollutants. Studies focused on biological (enzyme-based) methods have shown potential as greener and possibly more economical alternatives to other treatment approaches, such as chemical methods. The current study focused on the use of recombinantly produced novel bacterial peroxidases, namely dye-decolorizing peroxidases (DyPs), to study their effectiveness in degrading a number of diverse EPs. In this context, a sensitive bioanalytical Liquid chromatography—tandem mass spectrometry (LCMSMS)-based method was developed to simultaneously detect a mixture of 31 EPs and to examine their degradability by a panel of seven different recombinant bacterial DyPs (rDyPs). We show that up to 9 of the 31 tested EPs could be degraded by at least one of the DyPs tested. The results also indicated that not all rDyPs behaved similarly in their abilities to degrade EPs, as some rDyPs (such as SviDyP and CboDyP) showed a promising potential to degrade EPs while others (such as ScDyP) were almost ineffective. Additionally, the role of redox mediators for effective emerging pollutant degradation by rDyPs was also examined, which showed dramatic improvement in the DyP-mediated degradation of five different EPs. Detailed analysis of 2-mercaptobenzothiazole degradation by SviDyP showed that six distinct breakdown products were generated. The present study showed for the first time that recombinant bacterial DyPs can be used for wastewater remediation by degrading a range of different EPs.

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Section: Resultsmentioning

confidence: 56%

Efficient Degradation of 2-Mercaptobenzothiazole and Other Emerging Pollutants by Recombinant Bacterial Dye-Decolorizing Peroxidases

et al. 2021

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“…The transformation products observed in our current study are consistent with previous studies in which different biological methods (microbes and pure enzymes) were used to treat MBT. For example, the 166 m/z, 268 m/z and 284 m/z species reported here were also detected during MBT degradation in microbial electrolysis cells [ 40 ]. Additionally, the 136 m/z and 333 m/z transformation products have been previously reported during the degradation of MBT by pure soybean peroxidase as well as chloroperoxidase enzymes [ 37 ].…”

Section: Resultsmentioning

confidence: 98%

Efficient degradation of various emerging pollutants by wild type and evolved fungal DyP4 peroxidases

et al. 2022

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The accumulation of emerging pollutants in the environment remains a major concern as evidenced by the increasing number of reports citing their potential risk on environment and health. Hence, removal strategies of such pollutants remain an active area of investigation. One way through which emerging pollutants can be eliminated from the environment is by enzyme-mediated bioremediation. Enzyme-based degradation can be further enhanced via advanced protein engineering approaches. In the present study a sensitive and robust bioanalytical liquid chromatography-tandem mass spectrometry (LCMSMS)-based approach was used to investigate the ability of a fungal dye decolorizing peroxidase 4 (DyP4) and two of its evolved variants—that were previously shown to be H2O2 tolerant—to degrade a panel of 15 different emerging pollutants. Additionally, the role of a redox mediator was examined in these enzymatic degradation reactions. Our results show that three emerging pollutants (2-mercaptobenzothiazole (MBT), paracetamol, and furosemide) were efficiently degraded by DyP4. Addition of the redox mediator had a synergistic effect as it enabled complete degradation of three more emerging pollutants (methyl paraben, sulfamethoxazole and salicylic acid) and dramatically reduced the time needed for the complete degradation of MBT, paracetamol, and furosemide. Further investigation was carried out using pure MBT to study its degradation by DyP4. Five potential transformation products were generated during the enzymatic degradation of MBT, which were previously reported to be produced during different bioremediation approaches. The current study provides the first instance of the application of fungal DyP4 peroxidases in bioremediation of emerging pollutants.

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“…[ 142 ], Copyright 2021, Elsevier; c the compound 2-mercaptobenzothizaole (MBT) degradation product; d mechanistic routes proposed for the transformation of MBT under the reaction conditions; reproduced with permission from Ref. [ 143 ], Copyright 2020, Elsevier …”

Section: Cf Materials For Bessmentioning

confidence: 99%

Carbon Fibers for Bioelectrochemical: Precursors, Bioelectrochemical System, and Biosensors

Feng

et al. 2023

Adv. Fiber Mater.

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Carbon fibers (CFs) demonstrate a range of excellent properties including (but not limited to) microscale diameter, high hardness, high strength, light weight, high chemical resistance, and high temperature resistance. Therefore, it is necessary to summarize the application market of CFs. CFs with good physical and chemical properties stand out among many materials. It is believed that highly fibrotic CFs will play a crucial role. This review first introduces the precursors of CFs, such as polyacrylonitrile, bitumen, and lignin. Then this review introduces CFs used in BESs, such as electrode materials and modification strategies of MFC, MEC, MDC, and other cells in a large space. Then, CFs in biosensors including enzyme sensor, DNA sensor, immune sensor and implantable sensor are summarized. Finally, we discuss briefly the challenges and research directions of CFs application in BESs, biosensors and more fields. Highlights CF is a new-generation reinforced fiber with high hardness and strength. Summary precursors from different sources of CFs and their preparation processes. Introduction of the application and modification methods of CFs in BESs and biosensor. Suggest the challenges in the application of CFs in the field of bio-electrochemistry. Propose the prospective research directions for CFs. Graphical abstract

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Degradation of 2-mercaptobenzothizaole in microbial electrolysis cells: Intermediates, toxicity, and microbial communities

Cited by 19 publications

References 39 publications

Efficient Degradation of 2-Mercaptobenzothiazole and Other Emerging Pollutants by Recombinant Bacterial Dye-Decolorizing Peroxidases

Efficient Degradation of 2-Mercaptobenzothiazole and Other Emerging Pollutants by Recombinant Bacterial Dye-Decolorizing Peroxidases

Efficient degradation of various emerging pollutants by wild type and evolved fungal DyP4 peroxidases

Carbon Fibers for Bioelectrochemical: Precursors, Bioelectrochemical System, and Biosensors

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