Degradation of a Mixture of Hydrocarbons, Gasoline, and Diesel Oil Additives by
            <i>Rhodococcus aetherivorans</i>
            and
            <i>Rhodococcus wratislaviensis</i>

Auffret, Marc; Labbé, Diane; Thouand, Gérald; Greer, Charles W.; Fayolle-Guichard, Françoise

doi:10.1128/aem.01117-09

Cited by 93 publications

(66 citation statements)

References 44 publications

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“…T3-1. This N-deethoxymethylase was encoded by the ethABCD gene cluster involved in methyl tertbutyl ether (MTBE) and ETBE degradation (16)(17)(18)(19)(20)(21)(22)(23)(24). The functions of EthABCD in bacteria were genetically verified by gene complementation in mutant strains.…”

Section: Discussionmentioning

confidence: 99%

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Involvement of the Cytochrome P450 System EthBAD in the N -Deethoxymethylation of Acetochlor by Rhodococcus sp. Strain T3-1

Wang

Zhou

et al. 2015

Appl Environ Microbiol

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c Acetochlor [2-chloro-N-(ethoxymethyl)-N-(2-ethyl-6-methylphenyl)-acetamide] is a widely applied herbicide with potential carcinogenic properties. N-Deethoxymethylation is the key step in acetochlor biodegradation. N-Deethoxymethylase is a multicomponent enzyme that catalyzes the conversion of acetochlor to 2=-methyl-6=-ethyl-2-chloroacetanilide (CMEPA). Fast detection of CMEPA by a two-enzyme (N-deethoxymethylase-amide hydrolase) system was established in this research. Based on the fast detection method, a three-component enzyme was purified from Rhodococcus sp. strain T3-1 using ammonium sulfate precipitation and hydrophobic interaction chromatography. The molecular masses of the components of the purified enzyme were estimated to be 45, 43, and 11 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Based on the results of peptide mass fingerprint analysis, acetochlor N-deethoxymethylase was identified as a cytochrome P450 system, composed of a cytochrome P450 oxygenase (43-kDa component; EthB), a ferredoxin (45 kDa; EthA), and a reductase (11 kDa; EthD), that is involved in the degradation of methyl tert-butyl ether. The gene cluster ethABCD was cloned by PCR amplification and expressed in Escherichia coli BL21(DE3). Resting cells of a recombinant E. coli strain showed deethoxymethylation activity against acetochlor. Subcloning of ethABCD showed that ethABD expressed in E. coli BL21(DE3) has the activity of acetochlor N-deethoxymethylase and is capable of converting acetochlor to CMEPA. Chloroacetanilide herbicides are a class of important herbicides used worldwide. The chloroacetanilide herbicide acetochlor [2-chloro-N-(ethoxymethyl)-N-(2-ethyl-6-methylphenyl)-acetamide] is a selective preemergent herbicide that has been used to effectively control broadleaf weeds and annual grasses in corn fields for almost 40 years (1). The excessive and frequent application of acetochlor may result in high levels of acetochlor residues, which have been detected in ground and surface waters and negatively impact both the environment and agricultural ecosystems (2). The U.S. Environmental Protection Agency (EPA) has classified acetochlor as a B-2 carcinogen and a probable human carcinogen (3). Studies of different soil types treated with acetochlor have demonstrated that 2=-methyl-6=-ethyl-2-chloroacetanilide (CMEPA), a compound derived from acetochlor N-deethoxymethylation, is a major product of the microbial degradation process (1, 3-5).We previously isolated an efficient acetochlor N-deethoxymethylation strain, T3-1, from a microbial consortium that could mineralize acetochlor completely. The strain was identified as a Rhodococcus sp. (1). The biochemical pathway of acetochlor degradation by the three bacteria in the consortium was proposed to be as follows: acetochlor to CMEPA by Rhodococcus sp. strain T3-1, CMEPA to 2-methyl-6-ethyl aniline (MEA) by Delftia sp. strain T3-6, and MEA by Sphingobium sp. strain MEA3-1, based on the identified degradation intermediates (1). In this study, a three-...

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Section: Discussionmentioning

confidence: 99%

“…Many strains harboring the ethRABCD gene cluster have been reported, and its function was also studied by genetic methods (16)(17)(18)(19)(20)(21)(22)(23)(24). The ethABCD cluster encoded a ferredoxin reductase (EthA), a cytochrome P450 (EthB), a ferredoxin (EthC), and a protein with an unknown function (EthD) (17).…”

Section: Discussionmentioning

confidence: 99%

Involvement of the Cytochrome P450 System EthBAD in the N -Deethoxymethylation of Acetochlor by Rhodococcus sp. Strain T3-1

Wang

Zhou

et al. 2015

Appl Environ Microbiol

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“…Studies of xenobiotic-degrading bacteria have revealed that members of the genera Alicycliphilus, Polaromonas, and Rhodococcus are able to degrade cyclohexanol, which is related in its chemical structure to HDO (5,25,35). Whether such bacterial genera are able to cope with such defined habitat conditions (e.g., presence of copper compounds) and whether they are active key players in degrading preserved wood is questionable and has not been reported so far.…”

mentioning

confidence: 99%

Biodegradation of a Biocide (Cu- N -Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community

Jakobs-Schönwandt

Mathies

Abraham

et al. 2010

Appl Environ Microbiol

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The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, the fate of isotope-labeled ( 13 C) and nonlabeled ( 12 C) Cu-HDO incorporated in wood sawdust mixed with soil was investigated. HDO concentration was monitored by high-pressure liquid chromatography. The total carbon and the ␦ 13 C content of respired CO 2 , as well as of the soil-wood-sawdust mixture, were determined with an elemental analyzer-isotopic ratio mass spectrometer. The concentration of HDO decreased significantly after 105 days of incubation, and after 24 days the 13 CO 2 concentration respired from soil increased steadily to a maximum after 64 days of incubation. Phospholipid fatty acid-stable isotope probing (PFA-SIP) analysis revealed that the dominant PFAs C 19:0 d8,9, C 18:0 , C 18:1 7, C 18:2 6,9, C 17:1 d7,8, C 16:0 , and C 16:1 7 were highly enriched in their ␦ 13 C content. Moreover, RNA-SIP identified members of the phylum Acidobacteria and the genera Phenylobacterium and Comamonas that were assimilating carbon from HDO exclusively. Cu-HDO as part of a wood preservative effectively decreased fungal wood decay and overall microbial respiration from soil. In turn, a defined bacterial community was stimulated that was able to metabolize HDO completely.

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“…A fent bemutatott izolátumokon kívül egyéb törzsekről is leírták, hogy az éter típusú üzemanyag-oxigenátok bontására képesek [74,119,[137][138][139][140][141][142][143][144][145] …”

Section: áBra Az Etbe Metabolikus úTvonala a Rhodococcus Ruber Ifp 20unclassified

Éter típusú üzemanyag-adalékok mikrobiális bontása

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Degradation of a Mixture of Hydrocarbons, Gasoline, and Diesel Oil Additives by Rhodococcus aetherivorans and Rhodococcus wratislaviensis

Cited by 93 publications

References 44 publications

Involvement of the Cytochrome P450 System EthBAD in the N -Deethoxymethylation of Acetochlor by Rhodococcus sp. Strain T3-1

Involvement of the Cytochrome P450 System EthBAD in the N -Deethoxymethylation of Acetochlor by Rhodococcus sp. Strain T3-1

Biodegradation of a Biocide (Cu- N -Cyclohexyldiazenium Dioxide) Component of a Wood Preservative by a Defined Soil Bacterial Community

Éter típusú üzemanyag-adalékok mikrobiális bontása

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