Degradation of alkanes by bacteria

Rojo, Fernando

doi:10.1111/j.1462-2920.2009.01948.x

Cited by 688 publications

(506 citation statements)

References 138 publications

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“…This lag period was similar to the 5 to 8 day lag observed during other carbon enrichment experiments on polar bacterial communities (Dyda et al 2009;Ducklow et al 2011). Bacterial communities usually need time to activate the pathway of enzymes required for hydrocarbon degradation (Rojo 2009). The fast response to hydrocarbon exposure observed in our study may be due to a subpopulation of alkane degraders that is maintained by alkanes, which are ubiquitous in the marine environment, being continuously produced by many organisms (plants, green algae, bacteria and animals; Rojo 2009).…”

Section: Microbial Response To Oil Exposuresupporting

confidence: 53%

“…The limited biodegradation in sea ice may also be explained by the availability of highly labile dissolved organic matter produced by sea-ice algae (Amon et al 2001). Algal-derived carbon supports substantial bacterial growth (Riedel et al 2008), and most alkane-degrading bacteria were shown to prefer such labile compounds than alkanes when available (Rojo 2009). The very high DOC and chl a concentrations found in sea ice as well as the apparent absence of (or a short) lag period in sea-ice bacterial activity would agree with a bacterial production supported by sea-ice organic carbon.…”

Section: Microbial Response To Oil Exposurementioning

confidence: 99%

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Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada)

Garneau

Michel

Meisterhans

et al. 2016

FEMS Microbiology Ecology

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee AbstractThe increasing accessibility to navigation and offshore oil exploration brings risks of hydrocarbon releases in Arctic waters. Bioremediation of hydrocarbons is a promising mitigation strategy but challenges remain, particularly due to low microbial metabolic rates in cold, ice-covered seas.Hydrocarbon degradation potential of ice-associated microbes collected from the Northwest Passage was investigated. Microcosm incubations were run for 15 days at -1.7°C with and without oil to determine the effects of hydrocarbon exposure on microbial abundance, diversity and activity, and to estimate component-specific hydrocarbon loss. Diversity was assessed with automated ribosomal intergenic spacer analysis and ion torrent 16S rRNA gene sequencing. Bacterial activity was measured by 3 H-leucine uptake rates. After incubation, sub-ice and sea-ice communities degraded 94% and 48% of the initial hydrocarbons, respectively. Hydrocarbon exposure changed the composition of sea-ice and sub-ice communities; in sea-ice microcosms, Bacteroidetes (mainly Polaribacter) dominated whereas in sub-ice microcosms, Epsilonproteobacteria contribution increased, but that of Alphaproteobacteria and Bacteroidetes decreased. Sequencing data revealed a decline in diversity and increases in Colwellia and Moritella in oil-treated microcosms. Low concentration of dissolved organic matter (DOM) in sub-ice seawater may explain higher hydrocarbon degradation when compared to sea ice, where DOM was abundant and composed of labile exopolysaccharides.

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Section: Microbial Response To Oil Exposuresupporting

confidence: 53%

Section: Microbial Response To Oil Exposurementioning

confidence: 99%

Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada)

Garneau

Michel

Meisterhans

et al. 2016

FEMS Microbiology Ecology

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“…Catabolic degradation of hydrocarbons by marine bacteria produces significant amounts of CO 2 , which can be incorporated back into alkanes within cyanobacteria (33). Based on our estimates of cyanobacterial alkane production rates and observed concentrations in the ocean, we expect that this "short-term hydrocarbon cycle" (46) occurs on the order of days (Fig.…”

Section: Cyanobacterial Hydrocarbon Production Can Support Populationmentioning

confidence: 99%

“…Alkane degradation pathways have not been identified in grazers of cyanobacteria (33). Therefore, the majority of cyanobacterially produced hydrocarbons are likely to be released into the environment and subsequently degraded by bacteria (34).…”

Section: Both Obligate and Facultative Hydrocarbon-degrading Bacteriamentioning

confidence: 99%

Contribution of cyanobacterial alkane production to the ocean hydrocarbon cycle

Lea‐Smith

Biller

Davey

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

174

122

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Hydrocarbons are ubiquitous in the ocean, where alkanes such as pentadecane and heptadecane can be found even in waters minimally polluted with crude oil. Populations of hydrocarbon-degrading bacteria, which are responsible for the turnover of these compounds, are also found throughout marine systems, including in unpolluted waters. These observations suggest the existence of an unknown and widespread source of hydrocarbons in the oceans. Here, we report that strains of the two most abundant marine cyanobacteria, Prochlorococcus and Synechococcus, produce and accumulate hydrocarbons, predominantly C15 and C17 alkanes, between 0.022 and 0.368% of dry cell weight. Based on global population sizes and turnover rates, we estimate that these species have the capacity to produce 2-540 pg alkanes per mL per day, which translates into a global ocean yield of ∼308-771 million tons of hydrocarbons annually. We also demonstrate that both obligate and facultative marine hydrocarbon-degrading bacteria can consume cyanobacterial alkanes, which likely prevents these hydrocarbons from accumulating in the environment. Our findings implicate cyanobacteria and hydrocarbon degraders as key players in a notable internal hydrocarbon cycle within the upper ocean, where alkanes are continually produced and subsequently consumed within days. Furthermore we show that cyanobacterial alkane production is likely sufficient to sustain populations of hydrocarbon-degrading bacteria, whose abundances can rapidly expand upon localized release of crude oil from natural seepage and human activities.cyanobacteria | hydrocarbons | hydrocarbon cycle | hydrocarbon-degrading bacteria | oil remediation

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“…First, the spill released not just oil, but large amounts of natural gas (1,2). The bacteria that typically consume methane, the primary component of natural gas, have limited abilities to consume multicarbon substrates (3), whereas many oil degraders are capable of growth only on larger hydrocarbons (4), and none are known to consume methane (5). The organisms that consume ethane and propane, the other major components of natural gas, remain largely unknown in marine environments, but may include organisms related to methanotrophs or oil-degraders (6).…”

mentioning

confidence: 99%

Natural gas and temperature structured a microbial community response to the Deepwater Horizon oil spill

Redmond

Valentine

2011

Proc. Natl. Acad. Sci. U.S.A.

358

379

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Microbial communities present in the Gulf of Mexico rapidly responded to the Deepwater Horizon oil spill. In deep water plumes, these communities were initially dominated by members of Oceanospirillales, Colwellia, and Cycloclasticus. None of these groups were abundant in surface oil slick samples, and Colwellia was much more abundant in oil-degrading enrichment cultures incubated at 4°C than at room temperature, suggesting that the colder temperatures at plume depth favored the development of these communities. These groups decreased in abundance after the well was capped in July, but the addition of hydrocarbons in laboratory incubations of deep waters from the Gulf of Mexico stimulated Colwellia's growth. Colwellia was the primary organism that incorporated 13 C from ethane and propane in stable isotope probing experiments, and given its abundance in environmental samples at the time that ethane and propane oxidation rates were high, it is likely that Colwellia was active in ethane and propane oxidation in situ. Colwellia also incorporated 13 C benzene, and Colwellia's abundance in crude oil enrichments without natural gas suggests that it has the ability to consume a wide range of hydrocarbon compounds or their degradation products. However, the fact that ethane and propane alone were capable of stimulating the growth of Colwellia, and to a lesser extent, Oceanospirillales, suggests that high natural gas content of this spill may have provided an advantage to these organisms. methane | biodegradation | marine bacteria | archaea | alkane

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Degradation of alkanes by bacteria

Cited by 688 publications

References 138 publications

Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada)

Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada)

Contribution of cyanobacterial alkane production to the ocean hydrocarbon cycle

Natural gas and temperature structured a microbial community response to the Deepwater Horizon oil spill

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