Degradation of Cellulose by a Newly Isolated Mesophilic Anaerobe, Bacteroidaceae Family

Khan, Ayesha; Saddler, John N.; Patel, G. B.; Colvin, J. Ross; Martin, S. M.

doi:10.1111/j.1574-6941.1980.tb01574.x

Cited by 37 publications

(7 citation statements)

References 11 publications

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“…The second most dominant families were Fibrobacteraceae (12%) in the rumen and Bacteroidaceae (13%) in the manure samples. Members of the Bacteroidaceae family have been described to ferment cellulosic material and produce acetic acid and hydrogen [ 34 ]. The family Fibrobacteraceae (phylum: Fibrobacteres) includes two cultured species, Fibrobacter succinogenes and Fibrobacter intestinalis , which have been suggested to be the main cellulose degraders in ruminant gut systems [ 35 , 36 ].…”

Section: Resultsmentioning

confidence: 99%

Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters

Özbayram

İnce

et al. 2018

Microorganisms

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Cattle manure is frequently used as an inoculum for the start-up of agricultural biogas plants or as a co-substrate in the anaerobic digestion of lignocellulosic feedstock. Ruminal microbiota are considered to be effective plant fiber degraders, but the microbes contained in manure do not necessarily reflect the rumen microbiome. The aim of this study was to compare the microbial community composition of cow rumen and manure with respect to plant fiber-digesting microbes. Bacterial and methanogenic communities of rumen and manure samples were examined by 454 amplicon sequencing of bacterial 16S rRNA genes and mcrA genes, respectively. Rumen fluid samples were dominated by Prevotellaceae (29%), whereas Ruminococcaceae was the most abundant family in the manure samples (31%). Fibrobacteraceae (12%) and Bacteroidaceae (13%) were the second most abundant families in rumen fluid and manure, respectively. The high abundances of fiber-degrading bacteria belonging to Prevotellaceae and Fibrobacteraceae might explain the better performance of anaerobic digesters inoculated with rumen fluid. Members of the genus Methanobrevibacter were the predominant methanogens in the rumen fluid, whereas methanogenic communities of the manure samples were dominated by the candidate genus Methanoplasma. Our results suggest that inoculation or bioaugmentation with fiber-digesting rumen microbiota can enhance the anaerobic digestion of lignocellulosic biomass.

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Section: Resultsmentioning

confidence: 99%

Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters

Özbayram

İnce

et al. 2018

Microorganisms

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“…The genus Bacteroides belonging to the Bacteroidaceae family is the most abundant core component of the human gut microbiota (Karlsson et al ., 2011 ), where they are considered as carbohydrate-utilizing bacteria (Klocke et al ., 2007 ). In addition, members of the Bacteroidaceae family have been characterized as important cellulolytic microorganism (Khan et al ., 1980 ; Murray, 1986 ).…”

Section: Resultsmentioning

confidence: 99%

Mesophilic versus thermophilic anaerobic digestion of cattle manure: methane productivity and microbial ecology

Moset

Poulsen

Wahid

et al. 2015

Microbial Biotechnology

174

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In this study, productivity and physicochemical and microbiological (454 sequencing) parameters, as well as environmental criteria, were investigated in anaerobic reactors to contribute to the ongoing debate about the optimal temperature range for treating animal manure, and expand the general knowledge on the relation between microbiological and physicochemical process indicators. For this purpose, two reactor sizes were used (10 m3 and 16 l), in which two temperature conditions (35°C and 50°C) were tested. In addition, the effect of the hydraulic retention time was evaluated (16 versus 20 days).Thermophilic anaerobic digestion showed higher organic matter degradation (especially fiber), higher pH and higher methane (CH4) yield, as well as better percentage of ultimate CH4 yield retrieved and lower residual CH4 emission, when compared with mesophilic conditions. In addition, lower microbial diversity was found in the thermophilic reactors, especially for Bacteria, where a clear intensification towards Clostridia class members was evident.Independent of temperature, some similarities were found in digestates when comparing with animal manure, including low volatile fatty acids concentrations and a high fraction of Euryarchaeota in the total microbial community, in which members of Methanosarcinales dominated for both temperature conditions; these indicators could be considered a sign of process stability.

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“…The results indicated that maintaining the growth pH at 7.0 not only enabled complete substrate breakdown but also maintained a higher cell viability. Acetic acid is toxic to some micro-organisms (Hentges, 1967;Stewart, 1975) and it accumulates in anaerobic digesters operating under non-optimal conditions (McCarty, 1964). The inhibitory effect of volatile fatty acids is due to the undissociated form (Hentges, 1967;Meynell, 1963).…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

Effect of pH and Oxygen on Growth and Viability of Acetivibrio cellulolyticus

Breuil

Patel

1981

Microbiology

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Growth, hydrogen production and cellulose digestion by Acetivibrio cellulolyticus strain CD2 were considerably greater when the culture pH was maintained at 7-0 than when the pH was not controlled. Furthermore, if the pH of the growth medium was controlled the number of viable organisms was 6-fold greater after 3 d incubation and 100-fold greater after 6 d incubation compared with equivalent cultures in which the pH was not controlled. The differences were due to the combined effect of low pH values and acetic acid accumulation. The number of viable organisms was 2-to %fold lower after 12 h incubation in substrate-free medium containing 40 mM-acetic acid at pH 5 . 5 than in the same medium at pH 7.0. Addition of 90 mwacetic acid during growth in a cellobiose-containing medium lowered the growth rate by 30% and the rate of hydrogen production by 40%. Exposure of A . cellulolyticus to oxygen for up to 2 h did not affect viability measurements provided that the organisms were subsequently transferred to reduced media.

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Degradation of Cellulose by a Newly Isolated Mesophilic Anaerobe, Bacteroidaceae Family

Cited by 37 publications

References 11 publications

Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters

Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters

Mesophilic versus thermophilic anaerobic digestion of cattle manure: methane productivity and microbial ecology

Effect of pH and Oxygen on Growth and Viability of Acetivibrio cellulolyticus

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