Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase

Oldenhuis, Roelof; Vink, R.L.J.M.; Janssen, Dick B.; Witholt, Bernard

doi:10.1128/aem.55.11.2819-2826.1989

Cited by 397 publications

(244 citation statements)

References 21 publications

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“…The relative abundance of phototrophy and chloroplasts in the water column increased with the increase in C/N ratio, while the relative abundance of phototrophy in the sediment declined. Considering phototrophy in the water column was mainly associated with phytoplankton and cyanobacteria, these results are consistent with the report that supplementation of carbon source could improve growth of phytoplankton (Schrader, Green, & Perschbacher, 2011 & Witholt, 1989;Stoecker et al, 2006). In the present study, the relative abundance of Methylosinus, Crenothrix, Halomonas, Candidatus and Methylacidiphilum were sensitive to the change in C/N ratio.…”

Section: Discussionsupporting

confidence: 93%

The effect of C/N ratio on bacterial community and water quality in a mussel-fish integrated system

et al. 2018

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A 30‐day experiment was conducted to evaluate the effect of C/N ratio on water quality and bacterial community in an integrated system comprising one molluscan species (pearl mussel Hyriopsis cumingii) and two fish species (gibel carp Carassius gibelio and silver carp Hypophthalmichthys molitrix) at five C/N ratios (6, 8, 10, 12 and 14). The mussel and fishes were reared in the experimental tanks (400 L), but gibel carp received formulated feed. Water quality in the experimental tanks was analysed on day 0, 10, 20 and 30, and bacterial community in the water column and sediment was analysed on day 30. Total nitrogen, total phosphorus and total organic carbon accumulated in the tanks over time. Ammonia and nitrite decreased with the increase in C/N ratio. Bacterial community in the water column and sediment changed at the phylum and genus levels with the increase in C/N ratio, and the critical C/N ratio causing a functional shift of bacterial community occurred at 10 in water column and 12 in sediment. The increase in C/N ratio benefited the growth of both potential probiotics and pathogenic bacteria. The high C/N ratio enhanced the bacterial functions of chemoheterotrophy and hydrocarbon degradation, but depressed the functions of nitrification and denitrification in the water column and sediment respectively. This study reveals that the C/N ratio can be used as a tool to manipulate the bacterial community and water quality in the mussel‐fish integrated system.

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Section: Discussionsupporting

confidence: 93%

The effect of C/N ratio on bacterial community and water quality in a mussel-fish integrated system

et al. 2018

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“…Type II methanotrophs dominated in land¢ll soil which is in accordance with other studies [16,49] while a mixture of type I and type II methanotrophs was present in the wetland soil. The methanotrophs containing sMMO have a broad substrate speci¢city and are of special interest in degradation of halogenated hydrocarbons in land¢ll soils [10]. In this study we were able to identify a subunit of the gene for this enzyme with two di¡erent primer sets in three type II strains from the wetland soil and in 10 strains from the land¢ll soil.…”

Section: Discussionmentioning

confidence: 89%

Isolation of methane oxidising bacteria from soil by use of a soil substrate membrane system

Svenning¹,

Wartiainen²,

Hestnes³

et al. 2003

FEMS Microbiology Ecology

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A new method for isolation of methane oxidising bacteria (methanotrophs) is presented. Soil samples from a wetland area and a landfill were plated on polycarbonate membranes, which were incubated in a methane-air atmosphere using a non-sterile soil suspension as the medium. The membrane acted as a permeable growth support. The membrane method resulted in selective growth conditions, which allowed isolation of methane oxidising bacteria. The method resulted in isolation of both type I and type II methanotrophs from natural wetland and landfill soils. The isolates obtained from the landfill were dominated by type II methanotrophs and included several isolates carrying the gene for soluble methane monooxygenase (sMMO). Repetitive element sequence-based PCR fingerprinting documented genotypic diversity at the strain level. The presented method is a promising tool for easy and rapid isolation of different indigenous methanotrophs from an environment of interest.

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“…Such enzymes are produced by bacteria growing on e.g. methane [93,94], toluene [95,96], isoprene [97], p-cumene [98], propene [99], propane [100], or ammonia [101]. In many, but not all, such bacteria chlorinated ethylenes are converted co-metabolically.…”

Section: Aerobic Degradation Of Chlorinated Ethylenesmentioning

confidence: 99%

“…Epoxides are the products from the oxidation of cisand trans-dichloroethylene [93] and vinyl chloride [18], and by analogy probably also from trichloroethylene. The conversion of these epoxides is a critical step in the degradation route of chlorinated ethylenes.…”

Section: Aerobic Degradation Of Chlorinated Ethylenesmentioning

confidence: 99%

“…Other genetic engineering approaches are aimed at improving natural strains. TCE degradation activity in the methanotrophic organism Methylosinus trichosporium OB3b for example depends on the expression of a soluble methane monooxygenase [93]. This enzyme is only expressed under conditions of low copper availability.…”

Section: Aerobic Degradation Of Chlorinated Ethylenesmentioning

confidence: 99%

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Degradation of halogenated aliphatic compounds: The role of adaptation

Pries

Ploeg

Dolfing

et al. 1994

FEMS Microbiology Reviews

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A limited number of halogenated aliphatic compounds can serve as a growth substrate for aerobic microorganisms. Such cultures have (specifically) developed a variety of enzyme systems to degrade these compounds. Dehalogenations are of critical importance. Various heavily chlorinated compounds are not easily biodegraded, although there are no obvious biochemical or thermodynamic reasons why microorganisms should not be able to grow with any halogenated compound. The very diversity of catabolic enzymes present in cultures that degrade halogenated aliphatics and the occurrence of molecular mechanisms for genetic adaptation serve as good starting points for the evolution of catabolic pathways for compounds that are currently still resistant to biodegradation.

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Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase

Cited by 397 publications

References 21 publications

The effect of C/N ratio on bacterial community and water quality in a mussel-fish integrated system

The effect of C/N ratio on bacterial community and water quality in a mussel-fish integrated system

Isolation of methane oxidising bacteria from soil by use of a soil substrate membrane system

Degradation of halogenated aliphatic compounds: The role of adaptation

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