Degradation of Chloroaromatics: Purification and Characterization of a Novel Type of Chlorocatechol 2,3-Dioxygenase of
            <i>Pseudomonas putida</i>
            GJ31

Kaschabek, Stefan R.; Kasberg, T; Müller, Dagmar; Mars, Astrid E.; Janssen, Dick B.; Reineke, Walter

doi:10.1128/jb.180.2.296-302.1998

Cited by 107 publications

(37 citation statements)

References 52 publications

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“…The production of dioxygenase by this organism enables it to degrade 1,3-dichlorobenzene [16]. P. putida can degrade trichlorobenzenes [17] and chlorocatechols through the ortho or meta pathways [18]. These studies indicate that strains related to TR1 and TriRY produce enzymes capable of triclosan degradation.…”

Section: Resultsmentioning

confidence: 90%

“…The supernatant from the second centrifugation was then added to the initial supernatant and high pressure liquid chromatographic (HPLC) analysis performed. The Waters Breeze system with an Xterra RP 18 5 Wm particle diameter (4.6U150 mm) column (Waters, Milford, MA, USA) with a guard column was used. Samples were eluted isocratically with a mobile phase of 60 acetonitrile:40 water:0.5 acetic acid with a £ow rate of 1 ml min 31 (injection size 20 Wl).…”

Section: Chromatographic Analysis Of Triclosanmentioning

confidence: 99%

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Soil bacteria Pseudomonas putida and Alcaligenes xylosoxidans subsp. denitrificans inactivate triclosan in liquid and solid substrates

Meade

2001

FEMS Microbiology Letters

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Triclosan is a broad-spectrum antimicrobial agent that has been incorporated into many household and medical products. Bacteria with high levels of triclosan resistance were isolated from compost, water, and soil samples. Two of these bacteria, Pseudomonas putida TriRY and Alcaligenes xylosoxidans subsp. denitrificans TR1, were able to use triclosan as a sole carbon source and clear particulate triclosan from agar. A decrease in triclosan concentration was measured by HPLC within 6 h of inoculation with strain TriRY and 24 h with strain TR1. Bioassays demonstrated that triclosan was inactivated in liquid cultures and/or embedded in plastic by the growth of strain TriRY and strain TR1, permitting the growth of triclosan-sensitive bacteria. ß

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Section: Resultsmentioning

confidence: 90%

Section: Chromatographic Analysis Of Triclosanmentioning

confidence: 99%

Soil bacteria Pseudomonas putida and Alcaligenes xylosoxidans subsp. denitrificans inactivate triclosan in liquid and solid substrates

Meade

2001

FEMS Microbiology Letters

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“…Cat23DO of Burkholderiales can be phylogenetically grouped into three distinct clusters (Fig. 8), with the majority of them being related to Cat23DO CbzE of P. putida GJ31 (Kaschabek et al, 1998) or TbuE of R. pickettii PK01 (Kukor and Olsen, 1996) involved in chlorobenzene or toluene degradation (subfamily I.2.C according to the classification by Eltis and Bolin, 1996). However, the proteins of Limnobacter sp.…”

Section: Degradation Of Aromatic Compounds In Burkholderiales 1105mentioning

confidence: 99%

Genomic analysis of the potential for aromatic compounds biodegradation in Burkholderiales

Pérez‐Pantoja¹,

Donoso

Agulló

et al. 2011

Environmental Microbiology

269

155

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Summary The relevance of the β‐proteobacterial Burkholderiales order in the degradation of a vast array of aromatic compounds, including several priority pollutants, has been largely assumed. In this review, the presence and organization of genes encoding oxygenases involved in aromatics biodegradation in 80 Burkholderiales genomes is analysed. This genomic analysis underscores the impressive catabolic potential of this bacterial lineage, comprising nearly all of the central ring‐cleavage pathways reported so far in bacteria and most of the peripheral pathways involved in channelling of a broad diversity of aromatic compounds. The more widespread pathways in Burkholderiales include protocatechuate ortho ring‐cleavage, catechol ortho ring‐cleavage, homogentisate ring‐cleavage and phenylacetyl‐CoA ring‐cleavage pathways found in at least 60% of genomes analysed. In general, a genus‐specific pattern of positional ordering of biodegradative genes is observed in the catabolic clusters of these pathways indicating recent events in its evolutionary history. In addition, a significant bias towards secondary chromosomes, now termed chromids, is observed in the distribution of catabolic genes across multipartite genomes, which is consistent with a genus‐specific character. Strains isolated from environmental sources such as soil, rhizosphere, sediment or sludge show a higher content of catabolic genes in their genomes compared with strains isolated from human, animal or plant hosts, but no significant difference is found among Alcaligenaceae, Burkholderiaceae and Comamonadaceae families, indicating that habitat is more of a determinant than phylogenetic origin in shaping aromatic catabolic versatility.

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“…GP1, Comamonas testosteroni JH5 and BRC60 Sphingomonas putida GJ31 and Sphingomonas sp. BN6 have been isolated that transform chlorophenols via meta cleavage (Nakatsu & Wyndham, 1993;Wieser et al, 1994;Hollender et al, 1997;Koh et al, 1997;Kaschabek et al, 1998;Riegert et al, 1998;Mars et al, 1999). Environmentally, it remains unclear which of these pathways (ortho or meta) is used during in situ degradation.…”

Section: Introductionmentioning

confidence: 99%

Quantification of catechol dioxygenase gene expression in soil during degradation of 2,4-dichlorophenol

Lillis

Clipson

Doyle

2010

FEMS Microbiology Ecology

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The tfdC and C23O genes encode two catechol dioxygenases that catalyse ortho and meta cleavage of a key metabolite (chlorocatechol) of 2,4-dichlorophenol (2,4-DCP) metabolism, respectively. Primers were designed and a real-time PCR assay was developed to assess the abundance and expression of both tfdC and C23O genes in a soil amended with 2,4-DCP over a 21-day period. tfdC, the gene encoding the ortho cleaving dioxygenase, was significantly more abundant than the meta cleaving dioxygenase gene (C23O) throughout the experiment. The highest levels of tfdC were observed 2 days after amendment of soil with 2,4-DCP, at which stage the rate of 2,4-DCP degradation was at its maximum. In contrast, C230 copy numbers declined initially and peaked when degradation had slowed considerably. mRNA of the two chlorocatechol dioxygenase genes was not detected on day 0, but both genes were expressed after this time point. tfdC was expressed at a significantly higher level than C23O in 2,4-DCP-amended soil throughout the course of the microcosm, indicating the dominance of the ortho metabolic pathway. Phylogenetic analysis revealed a wide diversity of chlorocatechol dioxygenase genes in the 2,4-DCP-exposed soil examined.

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Degradation of Chloroaromatics: Purification and Characterization of a Novel Type of Chlorocatechol 2,3-Dioxygenase of Pseudomonas putida GJ31

Cited by 107 publications

References 52 publications

Soil bacteria Pseudomonas putida and Alcaligenes xylosoxidans subsp. denitrificans inactivate triclosan in liquid and solid substrates

Soil bacteria Pseudomonas putida and Alcaligenes xylosoxidans subsp. denitrificans inactivate triclosan in liquid and solid substrates

Genomic analysis of the potential for aromatic compounds biodegradation in Burkholderiales

Quantification of catechol dioxygenase gene expression in soil during degradation of 2,4-dichlorophenol

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