Degradation of chlortetracycline using immobilized laccase on Polyacrylonitrile-biochar composite nanofibrous membrane

Abstract

The continuous release of antibiotic compounds through wastewater effluent into environment has raised concerns about their potential problems for different organisms. Enzymatic degradation with laccase is a green option for removal of pharmaceutical compounds from aqueous media. In this study, laccase was immobilized onto homemade Polyacrylonitrile-biochar composite nanofibrous membrane and the obtained biocatalyst was employed for removal of chlortetracycline, a widely used antibiotic, from aqueous media in continuous mode. The results showed that the immobilized laccase has improved storage, temperature and pH stability compared to free laccase. Also, it retained more than 50% of its initial activity after 7cycles of ABTS oxidation which indicated improved enzyme reusability. Finally, while using immobilized laccase for degradation of chlortetracycline in continuous mode exhibited 58.3%, 40.7% and 22.6% chlortetracycline removal efficiency at flux rates of 1, 2 and 3mL/h∙cm.

Biochar-based materials and their applications in removal of organic contaminants from wastewater: state-of-the-art review

“…Many studies showed that the combined use of BCs with metal oxides/metal nanoparticles could achieve higher removal efficiency in removal of pharmaceutically active compounds (Table 3) (Shan et al 2016;Kim and Kan 2016;Taheran et al 2017). Zhou et al (2017) reported a novel iron and zinc co-doped sawdust BC (Fe/Zn biochar) and employed it to remove tetracycline (TET) from aqueous solution.…”

Application of biochar-based materials in environmental remediation: from multi-level structures to specific devices

“…Taking advantage of the membrane, good separation for alcohol and water mixture was also achieved (Lan et al 2016(Lan et al , 2018. Other applications of biochar-based membrane include controlled-release fertilizer (Zhou et al 2015), and enhanced degradation of organic pollutants by Absorb a wide range of organic solvents and oils with excellent recyclability and selectivity Wu et al (2013b) attaching enzyme (Taheran et al 2017). However, scalable, efficient and topical production of biochar membranes with tunable properties is essential for industrial applications to be feasible.…”

Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water

“…The pH of the solution may change the ionization state of the amino acids in the enzyme and affect its structure and activity, and even lead to its denaturation. 37,38 Hence, the activity proles of free laccase and laccase immobilized on MBC were investigated by performing activity assays at different pH values. As shown in Fig.…”