Degradation of citronellol, citronellal and citronellyl acetate by Pseudomonas mendocina IBPse 105

Abstract

Financial support: COREDES/FAPERGS, and D. Tozoni held a CAPES scholarship during the development of this work.Keywords: biodegradation, citronellol catabolism, monoterpenes degradation, P. mendocina. Abbreviations: GC-MS: gas chromatography-mass spectrometryThe purpose of this work was to stud the Terpenes or terpenoids are widespread in nature, and are biodegradation of citronellol, citronellal and citronellyl the most important component of the essential oil of many acetate by a soil Pseudomonas mendocina strain (IBPse higher plants (Loza-Tavera, 1999). These compounds are 105) isolated from a Cymbopogon windelandi field. This characterized by methyl-branched molecular structures strain efficiently used citronellol, citronellal, citronellyl derived from isoprene. Monoterpenes and their oxygenated acetate and myrcene as sole source of carbon, but was forms consist of two isoprene units (C10), and can be cyclic not able to grow on other 15 monoterpenoids evaluated.(1,8-cineole, camphor, etc.) or acyclic (citronellol, geraniol, Gas chromatography-mass spectrometry (GC-MS) citral, etc.). analysis of metabolites accumulation during P. medocina IBPse 105 growth on citronellol showed that Citronellol, citronellal and citronellyl acetate are among the this strain uses the citronellol catabolic pathway main constituent of the essential oils of several plants, like described for other species of the genus. IBPse 105 citrus, citronella grass, roses, lemongrass, basil, lemon degradation of citronellyl acetate initiates by its eucalyptus, among others, being responsible for the hydrolysis to citronellol. The mini-Tn5 insertion in aromatic and biological properties, including antibacterial mutant IBPse 105-303, impaired in citronellol activity, of these oils (Hammer et al. 1999; Dorman and degradation, but able to grow on citronellal, was located Deans. 2000;Bakkali et al. 2008). in a homologous of the P. aeruginosa atuB gene, that Acyclic terpenoids contain a 3-methyl substitution in the codifies citronellol deshydrogenase.

Characterization of cross-linked whey protein isolate-based films containingSatureja KhuzistanicaJamzad essential oil

“…p ‐Cymene produces swelling of the bacterial cell membrane and, thus, carvacrol probably may easily enter the bacterial cell (Aguirre et al, ; Ultee, Kets, Alberda, Hoekstra, & Smid, ). Decreasing size of the inhibition zones among the tested Negative‐ ( E. coli , S. typhi , Shigella dysenteria , and P. aeruginosa ) and positive‐ ( S. aureus , Bacillus ceruse , and S. faecium ) bacteria might be related to breakdown of monoterpens, since some microorganisms have potential to degrade terpenoid compounds and use them as sole carbon sources (Del Rosario, Tulin, & Estremos, ; Tozoni, Zacaria, Vanderlinde, Delamare, & Echeverrigaray, ).…”

Proteomic analysis suggests that monoterpenes in lemongrass disrupt Ca2+ homeostasis in Haemaphysalis longicornis leading to mitochondrial depolarization and cytotoxicity

“…The degradation of acyclic terpenoids has proven to be difficult due to the presence of 3‐methyl substitution in the principal hydrocarbon chain, although the reports of microorganisms with the capacity to degrade these compounds have elucidated their metabolisms [79]. Studies with citronellal degrading bacteria such as the Pseudomonas mendocina strain (IBPse 105) or P. citronellolis which can grow on citronellal, citronellyl acetate, citronellol, and myrcene as sole carbon source, have aided the understanding of the metabolism of monoterpenes [84]. Analysis of the accumulated metabolites during IBPse 105 growth revealed a citronellol catabolic pathway which involves the oxidation of citronellol to aldehyde, and then to the corresponding acid via the upper pathway.…”

“…Subsequently, the leucine catabolic pathway metabolizes the resultant metabolite to produce acetyl‐CoA and acetoacetate [79, 85–87]. This microbial catabolism of acyclic monoterpenes of the citronellol family (AMTC) has been attributed to two gene clusters – the atu cluster (acyclic terpene utilization), consisting of the atuCDEF genes are responsible for the lower pathway, whereas the liu cluster (leucine and isovalerate utilization), consisting of liuRABCDE genes are involved in the catabolism of monoterpenes and leucine [84, 87]. Similarly, KEGG pathway analysis in the present study revealed the activation of valine, leucine, and isoleucine degradation pathway with the involvement of about seven enzymes including 3‐oxoacid CoA‐transferase, acetyl‐CoA acetyltransferase, dihydrolipoamide transacylase, hydroxyacyl‐CoA dehydrogenase, medium‐chain acyl‐CoA dehydrogenase, pyruvate dehydrogenase, and short‐chain alcohol dehydrogenase.…”

Genome Analysis of a Limnobacter sp. Identified in an Anaerobic Methane-Consuming Cell Consortium

“…Citronellol contains a 3-methyl substitution, making degradation difficult. Only a few bacteria can use citronellol as the sole carbon source (Tozoni et al, 2010). Citronellol can be metabolized to acetyl coenzyme A and acetoacetate via the acyclic terpene utilization and leucine utilization pathways (Förster-Fromme et al, 2006).…”