Degradation of endocrine disrupting chemicals by genetic transformants with two lignin degrading enzymes in Phlebia tremellosa

Kum, Hyunwoo; Lee, Sungsuk; Ryu, Sun-Hwa; Choi, Hyoung T.

doi:10.1007/s12275-011-1230-y

Cited by 10 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Laccase (Lac), which exhibits a remarkable functional diversity, and especially Lac mediator systems, have gained a lot of attention due to their broad substrate specificity, which makes them useful in different industrial applications such as pulp delignification, waste detoxification, denim decolorization, and bioconversion of chemicals (Cañas and Camarero 2010;Mäkelä et al 2006;Villalba et al 2010). Among the innovative applications of ligninolytic enzyme associations, the successful combination between Lac and MnP of selected white-rot fungi has been recently described as a powerful tool for endocrine disrupting chemicals degradation and estrogenic activity removal (Kum et al 2011).…”

Section: Influence Of Culture Conditions On Laccase Production Growtmentioning

confidence: 99%

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

et al. 2013

View full text Add to dashboard Cite

aMany biotechnological processes pursuing sustainability aim for effective, inexpensive, and environmentally friendly alternatives to replace conventional practices. Laccase-containing lignocellulolytic systems from white rot fungi have been shown to be an efficient enzymatic tool for ecofriendly biological treatments. One objective of the biotechnological enzymes production process is to find optimum growing and secretion conditions for a selected fungus. In this work, different fungi isolated from the Misiones rainforest (Coriolus versicolor f. antarcticus BAFC-266, Ganoderma applanatum strain F, Phlebia brevispora BAFC-633, and Pycnoporus sanguineus BAFC-2126) were incubated at different temperatures (25, 29, 33 °C) and pH values (3.5, 4.5, 5.5) under static conditions for 7, 10, and 14 days to evaluate their growing ability and laccase (Lac) production. Results revealed specific favorable conditions for growth and protein secretion depending on the fungus under consideration, making it necessary to adjust these parameters for each particular case. The combined effect of these cultivation parameters showed a marked influence on the secreted Lac activity by P. brevispora BAFC 633, with the highest activity (~ 240 U/l) at 29 ºC and pH 4.5 at the 10 th day of cultivation. The presence of Lac isoenzymes also depended on the pH, temperature, and time of cultivation for the different tested fungi.

show abstract

Section: Influence Of Culture Conditions On Laccase Production Growtmentioning

confidence: 99%

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

et al. 2013

View full text Add to dashboard Cite

show abstract

“…DBP를 포함하는 프탈레이트류는 플라스틱 산업 에 널리 사용되며 그 독성이 매우 강한 것으로 알려져 있어 관심 이 높은 환경오염물질이다. 겨울우산버섯은 리그닌 분해효소를 분비해서 이러한 오염 물질을 분해할 수 있는 것으로 생각된다 (Lee et al, 2007;Ryu et al, 2008 (Yeo et al, 2007;Kum et al, 2011). 겨울우산 버섯은 100 µg/ml 이상 높은 농도의 phosphinothricin에서도 균사 가 성장하여 선택표지로 bar 유전자가 적합하지 않았다.…”

unclassified

“…최종 선별된 형질전환체는 벡터 1 µg 당 100-160개의 수율로 먹물버섯의 형질전환 수율(500-600개/µg DNA) 보다 낮으나 아 교버섯(15-25개/µg DNA)과 구름버섯(25-50개/µg DNA)의 형 질전환 수율보다 높았다 (Leem et al, 1999;Yeo et al, 2007;Kum et al, 2011). 본 실험에서는 형질전환 수율을 높이기 위해 heparin과 ATA 그리고 spermidine을 첨가하였는데 heparin은 벡터의 도입이 원활하도록 원형질체가 응집되는 것을 방지하며, ATA는 외래 유전자를 분해시키는 nuclease의 저해제로 형질전 환 과정에서 벡터 DNA가 분해되는 것을 막을 수 있으며, 양이 온을 띄는 spermidine은 음이온을 갖는 벡터 DNA를 원형질체 표면으로 붙여주기 때문에 벡터가 세포 내로 삽입되는 효율을 높이는 것으로 알려져 있다 (Hallick et al, 1977;Dickman 1988;Li et al, 2006) …”

unclassified

Protoplast Isolation and Genetic Transformation of Polyporus brumalis

Ryu¹,

Kim²

2014

The Korean Journal of Microbiology

View full text Add to dashboard Cite

This experiment was undertaken to investigate proper conditions for protoplast isolation and genetic transformation of the white rot fungi, Polyporus brumalis. The protoplasts were formed from mycelia at a frequency of 1 × 10 7 /ml with 0.5% Usukizyme. The transformation vector (pHYgpt) was constructed using hygromycin resistance gene (hph) for the selectable maker. The yield was 100-160 transformants/μg DNA in a transformation mediated by 40% polyethylene glycol solution with aurintricarboxylic acid, heparin and supermidine. The genomic integration of the pHYgpt was confirmed by hph-specific PCR and the expected amplified band appeared only in the transformants. These results could be an efficient tool in gene engineering of the genus polyporus. et al., 1993;Hatakka, 1994). 리그닌 분해효소는 기질특이성이 낮기 때문에 리그닌 외에 염료나 내분비계 장애물질 등 난분해성 유해화합물을 분해할 수 있으며, 분해과정이 세포외 에서 이루어지기 때문에 고농도의 독성화합물과 불용성화합물 의 분해도 가능하다 (Pasti-Grigsby et al., 1992;Barr and Aust, 1994;Pointing, 2001;Shin et al., 2007;Hwang et al., 2008

show abstract

Laccase production and pellet morphology of Coprinopsis cinerea transformants in liquid shake flask cultures

Rühl

Lange

Kües

2018

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Laccase production and pellet formation of transformants of Coprinopsis cinerea strain FA2222 of C. cinerea laccase gene lcc1 subcloned behind the gpdII-promoter from Agaricus bisporus were compared with a control transformant carrying no extra laccase gene. At the optimum growth temperature of 37 °C, maximal laccase yields of 2.9 U/ml were obtained by the best lcc1 transformant pYSK7-26 in liquid shake flask cultures. Reduction in temperature to 25 °C increased laccase yields up to 9.2 U/ml. The control transformant had no laccase activities at 37 °C but native activity at 25 °C (3.5 U/ml). Changing the temperature had severe effects on the morphology of the mycelial pellets formed during cultivation, but links of distinct pellet morphologies to native or recombinant laccase production could not be established. Automated image analysis was used to characterise pellet formation and morphological parameters (pellet area, diameter, convexity and mycelial structure). Cross sections of selected pellets showed that they differentiated in an outer rind and an inner medulla of loosened hyphae. Pellets at 25 °C had a small and dense outer zone and adopted with time a smooth surface. Pellets at 37 °C had a broader outer zone and a fringy surface due to generation of more and larger protuberances in the rind that when released can serve for production of further pellets.

show abstract

Degradation of endocrine disrupting chemicals by genetic transformants with two lignin degrading enzymes in Phlebia tremellosa

Cited by 10 publications

References 19 publications

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

Influence of Culture Conditions on Laccase Production, Growth, and Isoenzymes Patterns in Native White Rot Fungi from the Misiones Rainforest (Argentina)

Protoplast Isolation and Genetic Transformation of Polyporus brumalis

Laccase production and pellet morphology of Coprinopsis cinerea transformants in liquid shake flask cultures

Contact Info

Product

Resources

About