Degradation of eucalypt waste components by Lentinula edodes strains detected by chemical and near-infrared spectroscopy methods

Abstract

There are many changes, both qualitative and quantitative, in eucalypt waste during growth and fructification of Lentinula edodes. Wet chemical analysis and near-infrared (NIR) spectroscopy were used in conjunction with multivariate regression and principal components analysis to monitor biodegradation of eucalyptus waste during growth of several L. edodes strains. Weight and component losses of eucalypt residue after biodegradation by L. edodes strains were compared for periods of 1 to 5 mo. Decrease in cellulose, hemicellulose, and lignin contents occurred, however it was not concomitant. Measurement of lignin degradation by NIR and wet chemical analysis indicated its attack in the early stages of biodegradation. Selective lignin degradation by L. edodes was observed up to 2 mo of biodegradation for strains DEBIQ and FEB-14. One group of degraded substrate was identified based on the principal component analysis (PCA) of the data on their biodegradation time. Samples treated for 5 months by L. edodes strains (DEBIQ, UFV or FEB-14) differed from other, but no discrimination was observed among them. By the end of 5 mo, NIR analyses showed decrease of about 18-47% cellulose, 35-47% polyose and 39-60% lignin. These data were used for comparison with those obtained by wet chemical method for the degradation of the substrate by other five L. edodes strains cultivated at the same conditions. NIR calibration developed in this study was proven to be perfectly suitable as an analytical method to predict the changes in lignocellulose composition during biodegradation.

Influence of pretreatment severity on structural changes, lignin content and enzymatic hydrolysis of sugarcane bagasse samples

“…The enrichment of cellulose could probably be interfering with the intensity of this band. However, the band at 1730 cm À1 decreased due to the removal of arabinoxylan component (acetyl and uronic ester groups) after pretreatment [15,20]. Generally, the intensity of the bands between 1600 and 1300 increased with increasing pretreatment severity.…”

“…1A and were typical of the structure of sugarcane bagasse [7]. Principal component analysis (PCA) was applied to the FT-IR spectral data to allow for the discrimination of samples based on spectral information, and the identification of groups of samples that bear similarities or differences [20]. The PCA score plot depicts that samples were separated mainly on PC1 axis ( Fig.…”

Phenolic content and antioxidant activity in Lentinula edodes grown on eucalyptus biomass

“…Recent industrial developments have introduced local production of L edodes in the Ecuadorian highlands. Several studies elsewhere have reported variation in the composition de L edodes reflecting climatic changes and growth conditions (Pedri et al, 2015;Brienzo et al, 2007). Some of these variations may result in changes among secondary metabolites, as already reported for several enzymes (Pedri et al, 2015;Silva, 2008).…”