Degradation of food compounds and growth response on different food quality by the anaerobic ciliate Trimyema compressum

Holler, Stephan; Galle, Anne-Marie; Pfennig, Norbert

doi:10.1007/bf00248899

Cited by 3 publications

(3 citation statements)

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“…is two times less than on the strictly anaerobic Methanococcus thermolithotrophicus and the cell yield is ten times higher. Dependence of the velocity of growth on the food bacterium had been reported previously for T. compressum (Holler et al 1994; Yamada et al 1994). The culture conditions of ciliates feeding on Methanococcus are defined and easily controllable with what facilitates mass‐culturing.…”

Section: General Culture Requirementssupporting

confidence: 68%

“…The strictly mesophilic type species, T. compressum has been cultivated monoxenically (Goosen et al 1990a; Wagener and Pfennig 1987; Yamada et al 1994) and axenically (Broers et al 1991). Its growth rates were determined (Goosen et al 1990a) and its metabolism was studied (Goosen et al 1990b; Holler and Pfennig 1991; Holler et al 1994; Yamada et al 1994). In order to investigate the heat tolerance and growth temperature range from the Tyrrhenian Sea isolate, we cultured it and studied it physiologically.…”

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confidence: 99%

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Morphological, Small Subunit rRNA, and Physiological Characterization of Trimyema minutum (Kahl, 1931), an Anaerobic Ciliate from Submarine Hydrothermal Vents Growing from 28°C to 52°C

Baumgärtner

Stetter

Foissner

2002

J Eukaryotic Microbiology

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A thermophilic strain of Trimyema minutum was isolated from the hydrothermally heated sea floor at Vulcano Island (Italy) and cultivated monoxenically on Marinobacter sp. and Methanococcus thermolithotrophicus. It can be propagated strictly anaerobically and is sensitive to oxygen: if exposed to air at 48 degrees C all cells die within 60 min. It grows from 0.45-7.2% (w/v) salt and at pH 6.0-8.0. The isolate is the most extreme thermophilic ciliate which ever has been cultivated, exhibiting an optimal growth temperature of 48 degrees C (doubling time 6 h). Growth occurs between 28 degrees C and 52 degrees C. Trimyema minutum is redescribed using live observation and silver impregnation. Its morphology and the small subunit ribosomal RNA sequence is distinctly different from that of T. compressum, but morphology is highly similar to that of T. shoalsia Nerad et al. 1995, which is thus probably a junior synonym of T. minutum. To stabilize the bewildering species taxonomy in Trimyema, we suggest to recognize our population as a neotype of T. minutum.

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Section: General Culture Requirementssupporting

confidence: 68%

mentioning

confidence: 99%

Morphological, Small Subunit rRNA, and Physiological Characterization of Trimyema minutum (Kahl, 1931), an Anaerobic Ciliate from Submarine Hydrothermal Vents Growing from 28°C to 52°C

Baumgärtner

Stetter

Foissner

2002

J Eukaryotic Microbiology

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“…Previous findings suggested that the fermentation profile depends upon the bacterial species as prey, anaerobicity, and the existence of endosymbiotic methanogens [9, 11, 12]. T. compressum strain N, for example, produced a large amount of lactate when Bacteroides sp.…”

Section: Discussionmentioning

confidence: 99%

The effect of endosymbiotic methanogens on the growth and metabolic profile of the anaerobic free-living ciliate Trimyema compressum

Yamada

Kamagata

Nakamura

2006

FEMS Microbiology Letters

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We examined the influence of endosymbiotic methanogens on the growth and metabolic profile of the anaerobic ciliate Trimyema compressum. When we isolated the ciliate, it possessed a large number of endosymbiotic Methanobacterium‐like methanogens inside the cell. The culture was transferred to fresh medium with Lactobacillus sp. cells as food bacteria. T. compressum grew to give a maximum cell number of 3300 cells/ml without loss of the endosymbionts over 30 passages of transfer. Acetate and methane were major end products with smaller amounts of propionate and butyrate. However, after further continued cultivation, the number of endosymbiotic methanogens started to decrease and they eventually disappeared. The growth and metabolic profile of the ciliate were changed significantly as the symbiotic association deteriorated. The major fermentation products were shifted to butyrate. These results suggest that the endosymbiotic methanogens which function as hydrogen scavengers are not indispensable for the growth and survival of T. compressum, although the growth yield decreased slightly in the absence of methanogens, and the ciliate is capable of fermenting food bacteria without endosymbiotic methanogens by changing its metabolic pathway.

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Protozoa

Simon

Nerad

1996

Maintaining Cultures for Biotechnology and Industry

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Degradation of food compounds and growth response on different food quality by the anaerobic ciliate Trimyema compressum

Cited by 3 publications

References 16 publications

Morphological, Small Subunit rRNA, and Physiological Characterization of Trimyema minutum (Kahl, 1931), an Anaerobic Ciliate from Submarine Hydrothermal Vents Growing from 28°C to 52°C

Morphological, Small Subunit rRNA, and Physiological Characterization of Trimyema minutum (Kahl, 1931), an Anaerobic Ciliate from Submarine Hydrothermal Vents Growing from 28°C to 52°C

The effect of endosymbiotic methanogens on the growth and metabolic profile of the anaerobic free-living ciliate Trimyema compressum

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