Degradation of fructans by epiphytic and inoculated lactic acid bacteria and by plant enzymes during ensilage of normal and sterile hybrid ryegrass

Merry, R. J.; Winters, Ana L.; Thomas, PI; Müller, M. M.; Müller, Thomas

doi:10.1111/j.1365-2672.1995.tb00941.x

Cited by 39 publications

(46 citation statements)

References 26 publications

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“…That variation in the epiphytic population of microorganisms in the forage may be attributed to several factors, such as the temperature, ultraviolet radiation, and factors associated with the forage itself (Merry et al, 2008). In most of the studies, the number of epiphytic lactic bacteria is usually low at the moment that precedes the ensilage, but the population is elevated during the process (Lin et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

New inoculants on maize silage fermentation

et al. 2014

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-The objective of this study was to evaluate the effect of bacterial inoculants at two inoculation rates on chemical and biological characteristics of maize silage. The treatments consisted of two inoculating rates (5 and 6 log cfu g −1 of forage) for each strain of lactic acid bacteria (LAB) identified as Lactobacillus buchneri, L. hilgardii, or L. plantarum. The maize was ensiled in experimental PVC silos. Samples were taken for the determination of the contents of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), water-soluble carbohydrates (WSC), organic acids and alcohols, for the evaluation of the populations of lactic acid bacteria, yeasts, filamentous fungi, and for the determination of pH values during ensilage and after 30 or 90 days of fermentation. The doses of inoculants did not promote significant differences on the evaluated characteristics. There was effect of inoculants on acetic acid, 1.2-propanediol, LAB population, filamentous fungi, and pH value. No significant influence of the treatments with inoculants was observed in the variables DM, WSC, CP, lactic acid concentrations, or ethanol. The maximum temperature, i.e., the time to achieve the maximum temperature (TMT) and aerobic stability (AS), was not influencied by treatments. However, a decrease in maximum temperature, an increase in TMT, and improvement in the AS were observed after 90 days of fermentation. These results proved the advantage of microbial inoculation. The treatments influenced LAB populations and filamentous fungi, but no effect was observed on the yeast population. The best inoculation dose is 6 cfu g −1 of forage because it provides higher reduction of filamentous fungi in maize silage, thereby decreasing the aerobic deterioration by these microorganisms.

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Section: Discussionmentioning

confidence: 99%

New inoculants on maize silage fermentation

et al. 2014

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“…The resultant filtrate was then acidified with 0.5 ml H 3 PO 4 and stored at 4 1C for 24 h prior to freezing at À 20 1C. Samples were then defrosted and analysed for VFA and lactate content according to the method of Merry et al (1995).…”

Section: Faecal Vfa and Lactate Analysesmentioning

confidence: 99%

The Effect of Steaming and Soaking on the Respirable Particle, Bacteria, Mould, and Nutrient Content in Hay for Horses

Moore-Colyer

Taylor

James

2016

Journal of Equine Veterinary Science

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a b s t r a c tSeven mature Welsh-cross pony geldings provided the faecal inocula in a cross-over design experiment, consisting of two 14-day periods. In period 1, four ponies (group 1) were fed a low-starch fibre-mix (LS), and three (group 2) were fed a conventional highstarch coarse-mix (HS), both groups were fed these mixes in a 50:50 ratio with mature grass hay, to give a total daily dry matter (DM) intake of 17.5 g/kg live weight per day. Diets were then switched in period 2. At the end of each experimental period freshly voided faeces were collected from each animal and analysed for cellulolytic and amylolytic bacterial numbers, volatile fatty acid (VFA) and lactate concentration. There was no effect of diet on the number of cellulolytic and amylolytic bacteria, VFA or lactate present in the faeces of the experimental ponies. Consequently, it would appear that the effect of feeding LS or HS on faecal parameters is minimal; however, further work is required to determine the accuracy of faeces as a model for changes in the hindgut environment of the horse.

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“…Seward, London, UK). Numbers of lactic acid bacteria, enterobacteria, yeasts and moulds in herbage and silage extracts were determined as described by Merry et al (1995). No statistical analysis was completed on microbial count results because of lack of replicate data.…”

Section: Microbiological Analysis Of Herbage and Silagesmentioning

confidence: 99%

Effects of manipulating the protein content of white clover on silage quality

Winters

Minchin

Davies

et al. 2004

Animal Feed Science and Technology

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White clover nitrogen N content is generally high and constant throughout the growing season. It is also difficult to manipulate, as N 2 fixation tends to maintain a high N supply to the plant. White clover is a highly heterozygous, natural out-breeder, but self-fertile lines have recently been developed, including one which fails to develop nodules when grown under appropriate physiological conditions. An advantage of this line is that leaf protein content can be manipulated by altering the N supply to the plant and, in addition, it offers the opportunity to examine the effect of protein content on silage quality. This line was grown under varying levels of nitrate-N, giving rise to plants with a range of protein contents which were subsequently conserved as silage in two separate experiments. In experiment 1, both herbage protein and water-soluble carbohydrate (WSC) content varied, with values ranging from 17 to 31 g/kg dry matter (DM) and 52 to 72 g/kg DM, respectively. Protein content also varied in herbage grown for experiment 2, ranging from 20 to 33 g/kg DM, but variation in WSC content (91-101 g/kg DM) was not statistically significant. Silage quality was dependent on the protein content of the fresh herbage in both experiments. A comparison of herbages with high and low initial protein content, from experiments 1 and 2, respectively, revealed that a high protein content resulted in silages with a higher pH .L. Winters et al. / Animal Feed Science and Technology 116 (2004) 319-331 versus 659 g/kg TN), and an increase in free amino acid content when expressed as a proportion of total N (267 g/kg versus 90 g/kg and 323 g/kg versus 200 g/kg TN). A higher initial protein content results in a higher proportion of protein degradation during ensiling.

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Degradation of fructans by epiphytic and inoculated lactic acid bacteria and by plant enzymes during ensilage of normal and sterile hybrid ryegrass

Cited by 39 publications

References 26 publications

New inoculants on maize silage fermentation

New inoculants on maize silage fermentation

The Effect of Steaming and Soaking on the Respirable Particle, Bacteria, Mould, and Nutrient Content in Hay for Horses

Effects of manipulating the protein content of white clover on silage quality

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