Degradation of Humic Acids by the Litter-Decomposing Basidiomycete
            <i>Collybia dryophila</i>

Steffen, Kari; Hatakka, Annele; Hofrichter, Martin

doi:10.1128/aem.68.7.3442-3448.2002

Cited by 142 publications

(82 citation statements)

References 37 publications

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“…The increase in the laccase activity during the first 5 days correlated with the high colour removal during that period, suggesting involvement of laccase in the mycoremediation of HA. This is in agreement with previous studies under sterile conditions reporting on the degradation of humics by laccase [10,45].…”

Section: Removal Of Ha From Synthetic Wastewater By Immobilized Fungisupporting

confidence: 94%

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Zahmatkesh

Spanjers

Lier

2017

Environmental Technology

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Citation (APA)Zahmatkesh, M., Spanjers, H., & van Lier, J. B. (2017). A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum. Environmental Technology, 1-11. https://doi.org/10.1080/09593330.2017.1347718 Important noteTo cite this publication, please use the final published version (if applicable). Please check the document version above. CopyrightOther than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons. Takedown policyPlease contact us and provide details if you believe this document breaches copyrights. We will remove access to the work immediately and investigate your claim. In this study, we tested a new approach to facilitate the application of white rot fungi (WRF) under non-sterile conditions, by introducing grain sorghum as carrier and sole carbon and nutrient source for WRF. To this end, Trametes versicolor was immobilized on sorghum, and its ability to remove humic acid (HA) from synthetic and real industrial wastewater was studied. HA removal was measured as colour reduction and also analysed via size exclusion chromatography (SEC). Under sterile conditions, 80% colour removal was achieved for both synthetic and real wastewater using immobilized WRF on sorghum, without adding any additional carbon or nutrient sources. Under non-sterile conditions, immobilized fungi could again remove 80% of the colour and reached a maximum of 40 U/L laccase activity. In contrast, non-immobilized fungi cultivated in non-sterile wastewater supplemented with additional nutrients, reached only 10% decolourization and maximum 5 U/L laccase activity. SEC analysis showed that bioremoval of HA by WRF was associated with degradation of HA. Finally, immobilized fungi were used to treat real wastewater, under non-sterile conditions, in a sequential batch order without renewing the immobilized fungi. Four batch feedings were conducted and 80%, 70%, 50% and 40% colour removal was achieved for each batch, respectively, over a total incubation period of 19 days.

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Section: Removal Of Ha From Synthetic Wastewater By Immobilized Fungisupporting

confidence: 94%

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Zahmatkesh

Spanjers

Lier

2017

Environmental Technology

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“…Manganese is an important cofactor for oxidative enzymes, especially ones involved in lignin degradation. In other studies, manganese concentration was positively correlated with plant litter and humic acid decomposition rates and negatively correlated with the stable fraction of litter (Steffen et al, 2002;Berg et al, 2013). The greater effect of harvesting on edaphic factors in the organic versus mineral layer is consistent with greater effects of harvesting on the CAZy genes in the organic versus mineral layer (Figures 1c,4b and c).…”

Section: Edaphic Factorssupporting

confidence: 79%

Forest harvesting reduces the soil metagenomic potential for biomass decomposition

et al. 2015

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Soil is the key resource that must be managed to ensure sustainable forest productivity. Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition. From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb. A method to analyze unassembled metagenome reads from the complex community was optimized and validated. The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively. Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting. Gene families were disproportionately affected, and we identified 41 gene families consistently affected by harvesting, including families involved in lignin, cellulose, hemicellulose and pectin degradation. The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration. Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests.

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“…However, peroxidase activity is frequently measured in litter and soils, as an indicator of decomposition of recalcitrant organic matter (Sinsabaugh, 2005). Manganese peroxidases have been suggested to be involved in the degradation of humic compounds (Steffen et al, 2002). Detailed descriptions of the enzymes are restricted to a narrow phylogenetic range of polyporoid wood decomposers (for example, Phanerochaete chrysosporium, Trametes versicolor and Phlebia radiata) and Pleurotus spp.…”

Section: Introductionmentioning

confidence: 99%

ClassII peroxidase-encoding genes are present in a phylogenetically wide range of ectomycorrhizal fungi

Bödeker

Nygren

Taylor³

et al. 2009

The ISME Journal

160

123

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Fungal peroxidases (ClassII) have a key role in degrading recalcitrant polyphenolic compounds in boreal forest wood, litter and humus. To date, their occurrence and activity have mainly been studied in a small number of white-rot wood decomposers. However, peroxidase activity is commonly measured in boreal forest humus and mineral soils, in which ectomycorrhizal fungi predominate. Here, we used degenerate PCR primers to investigate whether peroxidase-encoding genes are present in the genomes of a wide phylogenetic range of ectomycorrhizal taxa. Cloning and sequencing of PCR products showed that ectomycorrhizal fungi from several different genera possess peroxidase genes. The new sequences represent four major homobasidiomycete lineages, but the majority is derived from Cortinarius, Russula and Lactarius. These genera are ecologically important, but consist mainly of non-culturable species from which little ecophysiological information is available. The amplified sequences contain conserved active sites, both for folding and substrate oxidation. In some Cortinarius spp., there is evidence for gene duplications during the evolution of the genus. ClassII peroxidases seem to be an ancient and a common feature of most homobasidiomycetes, including ectomycorrhizal fungi. Production of extracellular peroxidases may provide ectomycorrhizal fungi with access to nitrogen sequestered in complex polyphenolic sources.

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Degradation of Humic Acids by the Litter-Decomposing Basidiomycete Collybia dryophila

Abstract: The basidiomycete Collybia dryophila K209, which colonizes forest soil, was found to decompose a natural humic acid isolated from pine-forest litter (LHA) and a synthetic 14 C-labeled humic acid (

Cited by 142 publications

References 37 publications

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

A novel approach for application of white rot fungi in wastewater treatment under non-sterile conditions: immobilization of fungi on sorghum

Forest harvesting reduces the soil metagenomic potential for biomass decomposition

ClassII peroxidase-encoding genes are present in a phylogenetically wide range of ectomycorrhizal fungi

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