Degradation of Lignin in Agricultural Residues by locally Isolated Fungus Neurospora discreta

Pamidipati, Sirisha; Ahmed, Asma

doi:10.1007/s12010-016-2302-6

Cited by 28 publications

(16 citation statements)

References 28 publications

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“…Recently another mold Neurospora discrete was found to degrade lignin in sugarcane bagasse and produced nearly 1.5 times the amount of lignin degradation products in submerged culture. Based on this data, N. discrete is recorded to have high lignin degrading capability than previously reported lignin degrading fungi [55].…”

Section: White-rot Fungimentioning

confidence: 67%

Lignin Degradation by Fungal Pretreatment: A Review

Madadi¹,

Abbas²

2017

J Plant Pathol Microbiol

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Lignin is regarded as the most plentiful aromatic polymer contains both non-phenolic and phenolic structures. It makes the integral part of secondary wall and plays a significant role in water conduction in vascular plants. Many fungi, bacteria and insects have ability to decrease this lignin by producing enzymes. Among these fungi are the major player in degradation of lignin. These fungi produce enzymes such as lignin peroxidases and laccases. The well-known fungi which degrades lignin are white, brown, soft-rot fungi, and deuteromycetes. Currently these fungi are utilized to reduce lignin to produce ecofriendly bioenergy. The primary aim of this paper is to describe the lignin degradation by biological pre-treatment using fungi (white-, brown-soft-rot fungi and molds), as well as biochemical application. Besides, Molecular methods and enzymes regulation engaged in fungal pre-treatment and some of the factors affecting pretreatment will also be briefly discussed.

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Section: White-rot Fungimentioning

confidence: 67%

Lignin Degradation by Fungal Pretreatment: A Review

Madadi¹,

Abbas²

2017

J Plant Pathol Microbiol

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“…Cellulose and lignin contents were determined according to previous reports 17,18 . DRL and DRC were calculated according to previous reports 19,20 . SCC was determined referenced to a previous report 21 .…”

Section: Methodsmentioning

confidence: 99%

Degradation enhancement of rice straw by co-culture of Phanerochaete chrysosporium and Trichoderma viride

Chen

Tang

et al. 2019

Sci Rep

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Straw is one of the most abundant stock of renewable biomass from crop production. However, its utilization efficiency is still very low. Although co-cultivation of fungi increases the degrading rate, the co-cultivation condition needs to be optimized. To optimize the co-culture condition of Phanerochaete chrysosporium and Trichoderma viride degrading rice straw, we first tested the antagonistic characteristic between the fungi. The results showed that the best co-culture pattern was to first inoculate P. chrysosporium and culture for 4 days, then inoculate T. viride, and co-culture the two fungi for 4 days. The optimum fermentation condition was 14% (w/v) of inoculum concentration, the equivalent inoculation of the fungi, culture temperature at 30 °C, and 1:1.4 for solid-liquid ratio. Under the optimum condition, the degradation ratios of lignin and cellulose were 26.38% and 33.29%, respectively; the soluble carbon content in the culture product was 23.07% (w/v). The results would provide important reference information for the efficient utilization of rice straw to produce more accessible energy resources, such as ethanol and glucose.

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“…Neurospora discreta , isolated locally from the bark of a Subabul wood tree (Pamidipati and Ahmed ) was used for producing biofilms. N. discreta was maintained on potato dextrose agar (PDA) plates on which it grows as long filaments and produces abundant spores.…”

Section: Methodsmentioning

confidence: 99%

“…Three carbon sources were tested in this study: (i) sucrose, a disaccharide of glucose and fructose, and a common carbon source in used in microbial growth media due to the ease of degradation, (ii) cellulose, a natural plant‐derived polymer and a polysaccharide of β‐glucose units with 1,4‐glycosidic linkages and (iii) lignin, a bulky and recalcitrant phenolic polymer, consisting of several methoxylated derivatives of benzene (phenylpropanoid alcohols, also called monolignols). Lignin is a common by‐product of biorefineries based on lignocellulosic biomass as well as paper and pulp industries and is metabolized by very few organisms, including white rot fungi such as Phanaerochaete chrysosporium (Boyle et al ), as well as ascomycetes such as N. discreta (Pamidipati and Ahmed ). These carbon sources were selected as they represent three levels of relative complexity in structure, and differ in their molecular weights—all of which results in differences in how they are incorporated into the metabolic pathways of the fungus.…”

Section: Introductionmentioning

confidence: 99%

Influence of carbon source complexity on porosity, water retention and extracellular matrix composition ofNeurospora discretabiofilms

2019

Self Cite

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Aims To evaluate carbon source complexity as a process lever to impact the microstructure, chemical composition and water retention capacity of biofilms produced by Neurospora discreta. Methods and Results Biofilms were produced by nonpathogenic fungus N. discreta, using sucrose, cellulose or lignin as carbon source. The increase in complexity of carbon source from sucrose to lignin resulted in decreased water retention values (WRV) and wet weights of harvested biofilms. Confocal laser scanning microscopy was used to calculate porosity from bright‐field images, and relative stained areas of cells and carbohydrates from fluorescence imaging of samples stained with Trypan blue and Alexa Fluor 488. Porosity and relative quantity of cells increased with increase in carbon source complexity while the amount of carbohydrates decreased. The chemical analysis of the extracted extracellular matrix (ECM) showed that biofilms grown on more complex carbon sources had lower carbohydrate and protein content, which also explains the lower WRV trend, as carbohydrates are hydrophilic. Conclusions The nature of carbon source impacts the metabolic pathway of cells, thereby influencing the relative proportions of ECM and cells. This in turn impacts the microstructure, composition and water content of biofilms. Significance and Impact of the Study This work shows that carbon source can be used as process lever to control the properties of biofilms and presents a novel view of biofilms as potentially useful biomaterials.

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Degradation of Lignin in Agricultural Residues by locally Isolated Fungus Neurospora discreta

Cited by 28 publications

References 28 publications

Lignin Degradation by Fungal Pretreatment: A Review

Lignin Degradation by Fungal Pretreatment: A Review

Degradation enhancement of rice straw by co-culture of Phanerochaete chrysosporium and Trichoderma viride

Influence of carbon source complexity on porosity, water retention and extracellular matrix composition ofNeurospora discretabiofilms

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