Degradation of long-chain n-alkanes by a novel thermal-tolerant Rhodococcus strain

Xiang, Wei; Liang, Y. T.; Shen, Hong; Wang, Guan; You, Jianxin; Xue, Yanfen; Ma, Yanhe

doi:10.1007/s00203-022-02872-3

Cited by 11 publications

(9 citation statements)

References 30 publications

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“…Since the cell biomass is rather low in this water sample, we first concentrated it by using 0.22 µm hollow-fiber filter (MOF-1d, purchased from Motech Co. Ltd., Tianjin, China), and then, the filtrate was used for dilution and plating on the agar medium. The concentrated liquid was diluted with sterilized enrichment medium (ENM), plated on ENM agar, and incubated at 45°C ( 3 ). The pure strain was obtained by repeated streaking on the same medium agar plates.…”

Section: Announcementmentioning

confidence: 99%

Draft genome sequence ofDietziasp. strain CH92 isolated from oil reservoir

Xiang,

Zhang,

Wang

et al. 2024

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

Draft genome sequence ofDietziasp. strain CH92 isolated from oil reservoir

Xiang,

Zhang,

Wang

et al. 2024

Microbiol Resour Announc

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“…Rhodococcus spp. are widely distributed in various environments including contaminated soil, seawater and marine sediments, boreholes, groundwater, extreme conditions [38,28]. Moreover, this genus was reported to contain many members that can degrade different kinds of organic and xenobiotic compounds, such as alkane, polycyclic compounds, heterocyclic [39,40].…”

Section: Isolation and Identification Of The Diesel-degradingmentioning

confidence: 99%

“…The genome of R. qingshengii RHZ01 was 6,506,318 bp in total length, with only comprised one circular chromosome. The genome has 62.48% G+ C content which is near the recognized range for Rhodococcus species (62-69%) [28,41]. The genome was annotated with six databases (NR, Swiss-Prot, Pfam, EggNOG, Go and KEGG), which predicted a total of 6384 protein-coding sequences, 54 tRNAs, and 15 rRNAs.…”

Section: Genomic Characteristics Of the Isolate Rhz01mentioning

confidence: 99%

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Characterization of a Diesel-Degrading <i>Rhodococcus qingshengii</i> RHZ01 and Its Genome Analysis

Huang¹,

Wang²,

Zhang³

et al. 2022

Pol. J. Environ. Stud.

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Bioremediation is a low-cost, eco-friendly technique to reduce and/or eliminate pollutants from environment. Moreover, effective biological degrader for contaminants removal is important during bioremediation process. Here, the strain RHZ01, isolated from petroleum-contaminated soil in Shengli Oilfield, was identified as Rhodococcus qingshengii by the morphology, physiology, biochemistry and molecular biology. Strain RHZ01 was able to use diesel as the sole carbon source. The optimal initial pH and temperature for strain RHZ01 growth and diesel degradation were 6-8.5 and 20-30ºC, respectively. Nutrients (NaNO 3 , NH 4 Cl, KNO 3 and Urea) could replace NH 4 NO 3 . And Mg 2+ was required for strain RHZ01 growth and diesel degradation. To investigate the diesel degrading mechanisms of this strain, the complete genome was sequenced and annotated. The complete genome consists of one chromosome with a total length 6,506,318 bp and a G+C content of 62.48%. Five putative alkane monooxygenases (AlkB) encoding genes were further analyzed, and their protein sequences were characterized and compared with other published AlkBs in Rhodococcus spp.. The physicochemical features of the five AlkBs were analyzed, and these monooxygenase proteins are alkaline, unstable and thermostable. AlkB1, AlkB2 and AlkB3 are hydrophilic protein while the other proteins were hydrophobic. All of these alkane monooxygenases share several conserved regions, such as HYG-motif and three Hist boxes. From the results, it is demonstrated that bacterial strain isolated from petroleum-contaminated could be the potential bioremediation agent for diesel removal.

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“…The contributions of AlkB-type alkane hydroxylases to the long-chain alkane degradation are still far from clear. In a previous study, we isolated a novel Rhodococcus strain, CH91, with the ability to degrade long-chain n -alkanes, and two new alkB genes encoding alkane hydroxylase were annotated in the genome of this strain [ 22 ]. The enzymes encoded by the two alkB genes of strain CH91 had a lower identity with the well-characterized AlkB type alkane hydroxylases but showed significant similarity with the homologs from R. rhodochrous NCTC 10210, R. pyridinivorans DSM 44555, R. biphenylivorans TG9, and R. gordoniae NCTC13296, which belong to the R. rhodochrous subclade [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of Novel alkB Genes Encoding Long-Chain n-Alkane Hydroxylases in Rhodococcus sp. Strain CH91

et al. 2023

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Rhodococcus sp. strain CH91 is capable of utilizing long-chain n-alkanes as the sole carbon source. Two new genes (alkB1 and alkB2) encoding AlkB-type alkane hydroxylase were predicted by its whole-genome sequence analysis. The purpose of this study was to elucidate the functional role of alkB1 and alkB2 genes in the n-alkane degradation of strain CH91. RT-qPCR analyses revealed that the two genes were induced by n-alkanes ranging from C16 to C36 and the expression of the alkB2 gene was up-regulated much higher than that of alkB1. The knockout of the alkB1 or alkB2 gene in strain CH91 resulted in the obvious reduction of growth and degradation rates on C16-C36 n-alkanes and the alkB2 knockout mutant exhibited lower growth and degradation rate than the alkB1 knockout mutant. When gene alkB1 or alkB2 was heterologously expressed in Pseudomonas fluorescens KOB2Δ1, the two genes could restore its alkane degradation activity. These results demonstrated that both alkB1 and alkB2 genes were responsible for C16-C36 n-alkanes’ degradation of strain CH91, and alkB2 plays a more important role than alkB1. The functional characteristics of the two alkB genes in the degradation of a broad range of n-alkanes make them potential gene candidates for engineering the bacteria used for bioremediation of petroleum hydrocarbon contaminations.

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Degradation of long-chain n-alkanes by a novel thermal-tolerant Rhodococcus strain

Cited by 11 publications

References 30 publications

Draft genome sequence ofDietziasp. strain CH92 isolated from oil reservoir

Draft genome sequence ofDietziasp. strain CH92 isolated from oil reservoir

Characterization of a Diesel-Degrading <i>Rhodococcus qingshengii</i> RHZ01 and Its Genome Analysis

Functional Analysis of Novel alkB Genes Encoding Long-Chain n-Alkane Hydroxylases in Rhodococcus sp. Strain CH91

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