Degradation of myocardial high-energy phosphates during twenty-four hours of cold storage

Masuda, Mitsuharu; Sukehiro, S; Möllhoff, T.; Lü, Hua; Belle, H. Van; Flameng, Willem

doi:10.1016/s0022-5223(19)34924-4

Cited by 5 publications

(2 citation statements)

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“…This is consistent with a study on human m yocardium collected from patients undergoing heart transplantation. 13 In that study, the hearts of 8 patients were stored in ice-cold Ringer's solution for up to 24 h. In that period, the level of ATP decreased to 58% of the pre-ischemic value, but the major fraction of catabolites rem ained nucleotides. The total amount of nucleotides (ATP 1 ADP 1 AMP) decreased by only 20%.…”

Section: Discussionmentioning

confidence: 99%

Effects of Freeze-Thaw and Photobleaching on the Ultraviolet Resonance Raman Spectra of Human Colon Biopsies

et al. 2001

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We studied the effects of sample freezing and thawing, laser fluence, temperature, and oxygen on the ultraviolet resonance Raman (UVRR) spectra of human colon biopsies at 251 nm excitation. We show that the total adenylate content of the tissue decreases as a result of freezing and subsequent thawing to room temperature. We also show that photobleaching is the result of oxidative as well as thermal damage. Photobleaching consisted of a decrease in the intensity of the purine spectral bands and broadening and intensity increase of the aromatic amino acid bands. Both bio- and photodegradation of the tissue can be minimized by keeping the biopsy samples refrigerated during testing and the laser fluence under 0.2 mJ/μm2. Unlike amino acid photobleaching, purine photobleaching was also greatly reduced by placing the samples in an argon atmosphere. Sample storage could be achieved by refrigerating the fresh specimens and testing them within 30 h of surgical resection, without freezing. Alternatively, the fresh specimens could be snap-frozen in liquid nitrogen, then thawed and tested for up to 30 min. Altogether, these studies can be utilized to guide future experimental design and data interpretation during ultraviolet spectroscopy of biological tissues.

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Section: Discussionmentioning

confidence: 99%

Effects of Freeze-Thaw and Photobleaching on the Ultraviolet Resonance Raman Spectra of Human Colon Biopsies

et al. 2001

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“…Some reports pointed out that the ATP reduction rate showed two patterns: initially rapid and then slow. No explanation for these patterns was offered [14,15]. The other report suggested that this turning point indicated the reduction of organ viability [ 16].…”

Section: Discussionmentioning

confidence: 99%

University of Wisconsin solution versus modified collins solution for canine heart preservation: An experimental study

Sakata¹,

Kawashima²,

Ichikawa³

et al. 1997

Int J Angiol

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University of Wisconsin solution versus modified collins solution for canine heart preservation: An experimental study

et al. 2011

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Degradation of myocardial high-energy phosphates during twenty-four hours of cold storage

Cited by 5 publications

References 20 publications

Effects of Freeze-Thaw and Photobleaching on the Ultraviolet Resonance Raman Spectra of Human Colon Biopsies

Effects of Freeze-Thaw and Photobleaching on the Ultraviolet Resonance Raman Spectra of Human Colon Biopsies

University of Wisconsin solution versus modified collins solution for canine heart preservation: An experimental study

University of Wisconsin solution versus modified collins solution for canine heart preservation: An experimental study

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